Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures
The aim of this work was to determine PAs levels in pith tissues and callus cultures from haploid and diploid tobacco plants, explanted from the apical and basal regions of the stem. These explants were cultured in an RM-64 medium supplied with IAA and kinetin, under light or in the dark, during suc...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2010-04-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200020 |
| _version_ | 1818888333099532288 |
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| author | Sílvia Bicudo Carone Claudete Santa-Catarina Vanildo Silveira Eny Iochevet Segal Floh |
| author_facet | Sílvia Bicudo Carone Claudete Santa-Catarina Vanildo Silveira Eny Iochevet Segal Floh |
| author_sort | Sílvia Bicudo Carone |
| collection | DOAJ |
| description | The aim of this work was to determine PAs levels in pith tissues and callus cultures from haploid and diploid tobacco plants, explanted from the apical and basal regions of the stem. These explants were cultured in an RM-64 medium supplied with IAA and kinetin, under light or in the dark, during successive subcultures. PAs levels followed a basipetal decrease in diploid and an increase in haploid, pith tissues. A similar pattern of total PAs (free + conjugated) was observed for the callus of diploid and haploid plants maintained in the light, and for the haploid callus in the dark, whereas the diploid callus in the dark showed a constant increase in total PAs levels until the end of culture. The PA increase in the diploid callus in the dark was related to free Put levels increase. The ploidy status of the plants could express different PA gradients together with the plant pith and in vitro callus cultures.<br>O objetivo deste trabalho foi determinar os níveis de PAs em tecidos de medula e cultura de calos de plantas haplóides e diplóides de tabaco, obtidas da região apical e basal do caule. Estes explantes foram cultivados em meio RM-64 suplementado com AIA e cinetina, na luz e no escuro, durante vários subcultivos. Nos tecidos medulares, os níveis de PAs apresentam um decréscimo basípeto em diplóides e um aumento em haplóides.Um padrão similar nos níveis de PAs totais (livres+ conjugadas) foi observado em calos haplóides e diplóides mantidos na luz, e haplóides no escuro, enquanto os diplóides cultivados no escuro mostraram um aumento constante até o final do cultivo. O aumento no conteúdo de PAs nos calos diplóides no escuro, foi devido ao aumento do conteúdo de Put livre. Foi observado que a ploidia da planta pode expressar diferentes gradientes de PA ao longo do tecido medular e nas culturas de calos in vitro. |
| first_indexed | 2024-12-19T16:51:27Z |
| format | Article |
| id | doaj.art-f1076b0dbf9f47de9dd2175054fe57e2 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-19T16:51:27Z |
| publishDate | 2010-04-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-f1076b0dbf9f47de9dd2175054fe57e22022-12-21T20:13:32ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242010-04-0153240941710.1590/S1516-89132010000200020Polyamine patterns in haploid and diploid tobacco tissues and in vitro culturesSílvia Bicudo CaroneClaudete Santa-CatarinaVanildo SilveiraEny Iochevet Segal FlohThe aim of this work was to determine PAs levels in pith tissues and callus cultures from haploid and diploid tobacco plants, explanted from the apical and basal regions of the stem. These explants were cultured in an RM-64 medium supplied with IAA and kinetin, under light or in the dark, during successive subcultures. PAs levels followed a basipetal decrease in diploid and an increase in haploid, pith tissues. A similar pattern of total PAs (free + conjugated) was observed for the callus of diploid and haploid plants maintained in the light, and for the haploid callus in the dark, whereas the diploid callus in the dark showed a constant increase in total PAs levels until the end of culture. The PA increase in the diploid callus in the dark was related to free Put levels increase. The ploidy status of the plants could express different PA gradients together with the plant pith and in vitro callus cultures.<br>O objetivo deste trabalho foi determinar os níveis de PAs em tecidos de medula e cultura de calos de plantas haplóides e diplóides de tabaco, obtidas da região apical e basal do caule. Estes explantes foram cultivados em meio RM-64 suplementado com AIA e cinetina, na luz e no escuro, durante vários subcultivos. Nos tecidos medulares, os níveis de PAs apresentam um decréscimo basípeto em diplóides e um aumento em haplóides.Um padrão similar nos níveis de PAs totais (livres+ conjugadas) foi observado em calos haplóides e diplóides mantidos na luz, e haplóides no escuro, enquanto os diplóides cultivados no escuro mostraram um aumento constante até o final do cultivo. O aumento no conteúdo de PAs nos calos diplóides no escuro, foi devido ao aumento do conteúdo de Put livre. Foi observado que a ploidia da planta pode expressar diferentes gradientes de PA ao longo do tecido medular e nas culturas de calos in vitro.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200020biotechnologyNicotiana tabacumploidyputrescinespermidinespermine |
| spellingShingle | Sílvia Bicudo Carone Claudete Santa-Catarina Vanildo Silveira Eny Iochevet Segal Floh Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures Brazilian Archives of Biology and Technology biotechnology Nicotiana tabacum ploidy putrescine spermidine spermine |
| title | Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures |
| title_full | Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures |
| title_fullStr | Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures |
| title_full_unstemmed | Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures |
| title_short | Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures |
| title_sort | polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures |
| topic | biotechnology Nicotiana tabacum ploidy putrescine spermidine spermine |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200020 |
| work_keys_str_mv | AT silviabicudocarone polyaminepatternsinhaploidanddiploidtobaccotissuesandinvitrocultures AT claudetesantacatarina polyaminepatternsinhaploidanddiploidtobaccotissuesandinvitrocultures AT vanildosilveira polyaminepatternsinhaploidanddiploidtobaccotissuesandinvitrocultures AT enyiochevetsegalfloh polyaminepatternsinhaploidanddiploidtobaccotissuesandinvitrocultures |