PROSPECTS FOR APPLICATION OF BOVINE PERICARDIAL SCAFFOLD FOR CARDIAL SURGERY
The aim of the\* study was to estimate the properties of the scaffold obtained by decellularization of bovine pericardium with a 0.1% solution of sodium dodecyl sulfate. The experiment included standard histological, microscopic, molecular genetic, and biomechanical methods. Scaffold was tested in v...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-12-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/6_2020/sokol_6_2020.pdf |
| _version_ | 1797717645718454272 |
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| author | A. A. Sokol D. A. Grekov G. I. Yemets N. V. Shchotkina A. A. Dovghaliuk N. M. Rudenko I. M. Yemets |
| author_facet | A. A. Sokol D. A. Grekov G. I. Yemets N. V. Shchotkina A. A. Dovghaliuk N. M. Rudenko I. M. Yemets |
| author_sort | A. A. Sokol |
| collection | DOAJ |
| description | The aim of the\* study was to estimate the properties of the scaffold obtained by decellularization of bovine pericardium with a 0.1% solution of sodium dodecyl sulfate. The experiment included standard histological, microscopic, molecular genetic, and biomechanical methods. Scaffold was tested in vitro for cytotoxicity and in vivo for biocompatibility. A high degree of removal of cells and their components from bovine pericardium-derived matrix was shown. Biomechanical characteristics of artificial scaffold were the same as those of the native pericardium. With prolonged contact, no cytotoxic effect on human cells was observed. The biointegration of the scaffold in laboratory animals tissues was noted, which confirms the potential possibility of the implant applicationin cardiac surgery. |
| first_indexed | 2024-03-12T08:39:21Z |
| format | Article |
| id | doaj.art-f107a467fbf14e4eace91f920bfac682 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T08:39:21Z |
| publishDate | 2020-12-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-f107a467fbf14e4eace91f920bfac6822023-09-02T17:03:49ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2020-12-011364149doi.org/10.15407/biotech13.06.041PROSPECTS FOR APPLICATION OF BOVINE PERICARDIAL SCAFFOLD FOR CARDIAL SURGERYA. A. Sokol0D. A. Grekov1G. I. Yemets2N. V. Shchotkina3A. A. Dovghaliuk4N. M. Rudenko5 I. M. Yemets6State Institution "Scientific - Practical Medical Center of Pediatric Cardiology and Cardiac Surgery of the Ministry of Health of Ukraine", KyivState Institution "Scientific - Practical Medical Center of Pediatric Cardiology and Cardiac Surgery of the Ministry of Health of Ukraine", KyivState Institution "Scientific - Practical Medical Center of Pediatric Cardiology and Cardiac Surgery of the Ministry of Health of Ukraine", KyivState Institution "Scientific - Practical Medical Center of Pediatric Cardiology and Cardiac Surgery of the Ministry of Health of Ukraine", KyivState Institution "Scientific - Practical Medical Center of Pediatric Cardiology and Cardiac Surgery of the Ministry of Health of Ukraine", KyivState Institution "Scientific - Practical Medical Center of Pediatric Cardiology and Cardiac Surgery of the Ministry of Health of Ukraine", KyivState Institution "Scientific - Practical Medical Center of Pediatric Cardiology and Cardiac Surgery of the Ministry of Health of Ukraine", KyivThe aim of the\* study was to estimate the properties of the scaffold obtained by decellularization of bovine pericardium with a 0.1% solution of sodium dodecyl sulfate. The experiment included standard histological, microscopic, molecular genetic, and biomechanical methods. Scaffold was tested in vitro for cytotoxicity and in vivo for biocompatibility. A high degree of removal of cells and their components from bovine pericardium-derived matrix was shown. Biomechanical characteristics of artificial scaffold were the same as those of the native pericardium. With prolonged contact, no cytotoxic effect on human cells was observed. The biointegration of the scaffold in laboratory animals tissues was noted, which confirms the potential possibility of the implant applicationin cardiac surgery.http://biotechnology.kiev.ua/images/storage/6_2020/sokol_6_2020.pdfscaffolddecellularizationcardioimplanttissue engineering. |
| spellingShingle | A. A. Sokol D. A. Grekov G. I. Yemets N. V. Shchotkina A. A. Dovghaliuk N. M. Rudenko I. M. Yemets PROSPECTS FOR APPLICATION OF BOVINE PERICARDIAL SCAFFOLD FOR CARDIAL SURGERY Biotechnologia Acta scaffold decellularization cardioimplant tissue engineering. |
| title | PROSPECTS FOR APPLICATION OF BOVINE PERICARDIAL SCAFFOLD FOR CARDIAL SURGERY |
| title_full | PROSPECTS FOR APPLICATION OF BOVINE PERICARDIAL SCAFFOLD FOR CARDIAL SURGERY |
| title_fullStr | PROSPECTS FOR APPLICATION OF BOVINE PERICARDIAL SCAFFOLD FOR CARDIAL SURGERY |
| title_full_unstemmed | PROSPECTS FOR APPLICATION OF BOVINE PERICARDIAL SCAFFOLD FOR CARDIAL SURGERY |
| title_short | PROSPECTS FOR APPLICATION OF BOVINE PERICARDIAL SCAFFOLD FOR CARDIAL SURGERY |
| title_sort | prospects for application of bovine pericardial scaffold for cardial surgery |
| topic | scaffold decellularization cardioimplant tissue engineering. |
| url | http://biotechnology.kiev.ua/images/storage/6_2020/sokol_6_2020.pdf |
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