Transcriptionally inactive hepatitis B virus episome DNA preferentially resides in the vicinity of chromosome 19 in 3D host genome upon infection
Summary: The hepatitis B virus (HBV) infects 257 million people worldwide. HBV infection requires establishment and persistence of covalently closed circular (ccc) DNA, a viral episome, in nucleus. Here, we study cccDNA spatial localization in the 3D host genome by using chromosome conformation capt...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124721006598 |
| _version_ | 1818576229520900096 |
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| author | Dingbin Tang Hanqing Zhao Yumeng Wu Bo Peng Zhenchao Gao Yinyan Sun Jinzhi Duan Yonghe Qi Yunfei Li Zhongmin Zhou Guilan Guo Yu Zhang Cheng Li Jianhua Sui Wenhui Li |
| author_facet | Dingbin Tang Hanqing Zhao Yumeng Wu Bo Peng Zhenchao Gao Yinyan Sun Jinzhi Duan Yonghe Qi Yunfei Li Zhongmin Zhou Guilan Guo Yu Zhang Cheng Li Jianhua Sui Wenhui Li |
| author_sort | Dingbin Tang |
| collection | DOAJ |
| description | Summary: The hepatitis B virus (HBV) infects 257 million people worldwide. HBV infection requires establishment and persistence of covalently closed circular (ccc) DNA, a viral episome, in nucleus. Here, we study cccDNA spatial localization in the 3D host genome by using chromosome conformation capture-based sequencing analysis and fluorescence in situ hybridization (FISH). We show that transcriptionally inactive cccDNA is not randomly distributed in host nucleus. Rather, it is preferentially accumulated at specialized areas, including regions close to chromosome 19 (chr.19). Activation of the cccDNA is apparently associated with its re-localization, from a pre-established heterochromatin hub formed by 5 regions of chr.19 to transcriptionally active regions formed by chr.19 and nearby chromosomes including chr.16, 17, 20, and 22. This active versus inactive positioning at discrete regions of the host genome is primarily controlled by the viral HBx protein and by host factors including the structural maintenance of chromosomes protein 5/6 (SMC5/6) complex. |
| first_indexed | 2024-12-16T06:10:42Z |
| format | Article |
| id | doaj.art-f10902dffb3d412496b93589006a0e64 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-16T06:10:42Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-f10902dffb3d412496b93589006a0e642022-12-21T22:41:24ZengElsevierCell Reports2211-12472021-06-013513109288Transcriptionally inactive hepatitis B virus episome DNA preferentially resides in the vicinity of chromosome 19 in 3D host genome upon infectionDingbin Tang0Hanqing Zhao1Yumeng Wu2Bo Peng3Zhenchao Gao4Yinyan Sun5Jinzhi Duan6Yonghe Qi7Yunfei Li8Zhongmin Zhou9Guilan Guo10Yu Zhang11Cheng Li12Jianhua Sui13Wenhui Li14Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing, China; National Institute of Biological Sciences, Beijing, ChinaNational Institute of Biological Sciences, Beijing, ChinaPeking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing, China; National Institute of Biological Sciences, Beijing, ChinaPeking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing, China; National Institute of Biological Sciences, Beijing, ChinaNational Institute of Biological Sciences, Beijing, ChinaNational Institute of Biological Sciences, Beijing, ChinaNational Institute of Biological Sciences, Beijing, ChinaNational Institute of Biological Sciences, Beijing, ChinaNational Institute of Biological Sciences, Beijing, ChinaCollege of Life Sciences, Beijing Normal University, Beijing, China; National Institute of Biological Sciences, Beijing, ChinaCollege of Life Sciences, Beijing Normal University, Beijing, China; National Institute of Biological Sciences, Beijing, ChinaNational Institute of Biological Sciences, Beijing, ChinaSchool of Life Sciences, Center for Statistical Science, Center for Bioinformatics, Peking University, Beijing, ChinaNational Institute of Biological Sciences, Beijing, China; Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, ChinaNational Institute of Biological Sciences, Beijing, China; Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, China; Corresponding authorSummary: The hepatitis B virus (HBV) infects 257 million people worldwide. HBV infection requires establishment and persistence of covalently closed circular (ccc) DNA, a viral episome, in nucleus. Here, we study cccDNA spatial localization in the 3D host genome by using chromosome conformation capture-based sequencing analysis and fluorescence in situ hybridization (FISH). We show that transcriptionally inactive cccDNA is not randomly distributed in host nucleus. Rather, it is preferentially accumulated at specialized areas, including regions close to chromosome 19 (chr.19). Activation of the cccDNA is apparently associated with its re-localization, from a pre-established heterochromatin hub formed by 5 regions of chr.19 to transcriptionally active regions formed by chr.19 and nearby chromosomes including chr.16, 17, 20, and 22. This active versus inactive positioning at discrete regions of the host genome is primarily controlled by the viral HBx protein and by host factors including the structural maintenance of chromosomes protein 5/6 (SMC5/6) complex.http://www.sciencedirect.com/science/article/pii/S2211124721006598HBV3D host genomespatial localizationchromosome 19episomal DNAHBx protein |
| spellingShingle | Dingbin Tang Hanqing Zhao Yumeng Wu Bo Peng Zhenchao Gao Yinyan Sun Jinzhi Duan Yonghe Qi Yunfei Li Zhongmin Zhou Guilan Guo Yu Zhang Cheng Li Jianhua Sui Wenhui Li Transcriptionally inactive hepatitis B virus episome DNA preferentially resides in the vicinity of chromosome 19 in 3D host genome upon infection Cell Reports HBV 3D host genome spatial localization chromosome 19 episomal DNA HBx protein |
| title | Transcriptionally inactive hepatitis B virus episome DNA preferentially resides in the vicinity of chromosome 19 in 3D host genome upon infection |
| title_full | Transcriptionally inactive hepatitis B virus episome DNA preferentially resides in the vicinity of chromosome 19 in 3D host genome upon infection |
| title_fullStr | Transcriptionally inactive hepatitis B virus episome DNA preferentially resides in the vicinity of chromosome 19 in 3D host genome upon infection |
| title_full_unstemmed | Transcriptionally inactive hepatitis B virus episome DNA preferentially resides in the vicinity of chromosome 19 in 3D host genome upon infection |
| title_short | Transcriptionally inactive hepatitis B virus episome DNA preferentially resides in the vicinity of chromosome 19 in 3D host genome upon infection |
| title_sort | transcriptionally inactive hepatitis b virus episome dna preferentially resides in the vicinity of chromosome 19 in 3d host genome upon infection |
| topic | HBV 3D host genome spatial localization chromosome 19 episomal DNA HBx protein |
| url | http://www.sciencedirect.com/science/article/pii/S2211124721006598 |
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