Characterization of Bacillus amyloliquefaciens DA12 Showing Potent Antifungal Activity against Mycotoxigenic Fusarium Species
In an attempt to develop a biological control agent against mycotoxigenic Fusarium species, we isolated Bacillus amyloliquefaciens strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic F. asiaticum, F. graminearum, F. proliferatum, and F...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2017-10-01
|
| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624492 |
| _version_ | 1818935638791028736 |
|---|---|
| author | Theresa Lee Dami Park Kihyun Kim Seong Mi Lim Nan Hee Yu Sosoo Kim Hwang-Yong Kim Kyu Seok Jung Ja Yeong Jang Jong-Chul Park Hyeonheui Ham Soohyung Lee Sung Kee Hong Jin-Cheol Kim |
| author_facet | Theresa Lee Dami Park Kihyun Kim Seong Mi Lim Nan Hee Yu Sosoo Kim Hwang-Yong Kim Kyu Seok Jung Ja Yeong Jang Jong-Chul Park Hyeonheui Ham Soohyung Lee Sung Kee Hong Jin-Cheol Kim |
| author_sort | Theresa Lee |
| collection | DOAJ |
| description | In an attempt to develop a biological control agent against mycotoxigenic Fusarium species, we isolated Bacillus amyloliquefaciens strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic F. asiaticum, F. graminearum, F. proliferatum, and F. verticillioides both in vitro and in planta (maize). Further screening using dual culture extended the activity range of strain DA12 against other fungal pathogens including Botrytis cinerea, Colletotrichum coccodes, Endothia parasitica, Fusarium oxysporum, Raffaelea quercus-mongolicae, and Rhizoctonia solani. The butanol extract of the culture filtrate of B. amyloliquefaciens DA12 highly inhibited the germination of F. graminearum macroconidia with inhibition rate 83% at a concentration of 31.3 μg/ml and 100% at a concentration of 250 μg/ml. The antifungal metabolite from the butanol extract was identified as iturin A by thin layer chromatography-bioautography. In addition, volatile organic compounds produced by DA12 were able to inhibit mycelial growth of various phytopathogenic fungi. The volatile compounds were identified as 2-heptanone, 5-methyl heptanone and 6-methyl heptanone by gas chromatography-mass spectrometry (GC-MS) analysis. These results indicate that the antagonistic activity of Bacillus amyloliquefaciens DA12 was attributable to iturin A and volatile heptanones, and the strain could be used as a biocontrol agent to reduce the development of Fusarium diseases and mycotoxin contamination of crops. |
| first_indexed | 2024-12-20T05:23:21Z |
| format | Article |
| id | doaj.art-f10ac9ef29c84d43bb1b4820be872d72 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-20T05:23:21Z |
| publishDate | 2017-10-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-f10ac9ef29c84d43bb1b4820be872d722022-12-21T19:51:58ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542017-10-0133549950710.5423/PPJ.FT.06.2017.0126PPJ.FT.06.2017.0126Characterization of Bacillus amyloliquefaciens DA12 Showing Potent Antifungal Activity against Mycotoxigenic Fusarium SpeciesTheresa Lee0Dami Park1Kihyun Kim2Seong Mi Lim3Nan Hee Yu4Sosoo Kim5Hwang-Yong Kim6Kyu Seok Jung7Ja Yeong Jang8Jong-Chul Park9Hyeonheui Ham10Soohyung Lee11Sung Kee Hong12Jin-Cheol Kim13Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, KoreaMicrobial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, KoreaDivision of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, KoreaDivision of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, KoreaDivision of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, KoreaMicrobial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, KoreaMicrobial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, KoreaMicrobial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, KoreaMicrobial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, KoreaCrop Breeding Division, National Institute of Crop Science, Rural Development Administration (RDA), Wanju 55365, KoreaMicrobial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, KoreaMicrobial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, KoreaMicrobial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, KoreaDivision of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, KoreaIn an attempt to develop a biological control agent against mycotoxigenic Fusarium species, we isolated Bacillus amyloliquefaciens strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic F. asiaticum, F. graminearum, F. proliferatum, and F. verticillioides both in vitro and in planta (maize). Further screening using dual culture extended the activity range of strain DA12 against other fungal pathogens including Botrytis cinerea, Colletotrichum coccodes, Endothia parasitica, Fusarium oxysporum, Raffaelea quercus-mongolicae, and Rhizoctonia solani. The butanol extract of the culture filtrate of B. amyloliquefaciens DA12 highly inhibited the germination of F. graminearum macroconidia with inhibition rate 83% at a concentration of 31.3 μg/ml and 100% at a concentration of 250 μg/ml. The antifungal metabolite from the butanol extract was identified as iturin A by thin layer chromatography-bioautography. In addition, volatile organic compounds produced by DA12 were able to inhibit mycelial growth of various phytopathogenic fungi. The volatile compounds were identified as 2-heptanone, 5-methyl heptanone and 6-methyl heptanone by gas chromatography-mass spectrometry (GC-MS) analysis. These results indicate that the antagonistic activity of Bacillus amyloliquefaciens DA12 was attributable to iturin A and volatile heptanones, and the strain could be used as a biocontrol agent to reduce the development of Fusarium diseases and mycotoxin contamination of crops.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624492antifungal activityBacillus amyloliquefaciensiturin Amycotoxin |
| spellingShingle | Theresa Lee Dami Park Kihyun Kim Seong Mi Lim Nan Hee Yu Sosoo Kim Hwang-Yong Kim Kyu Seok Jung Ja Yeong Jang Jong-Chul Park Hyeonheui Ham Soohyung Lee Sung Kee Hong Jin-Cheol Kim Characterization of Bacillus amyloliquefaciens DA12 Showing Potent Antifungal Activity against Mycotoxigenic Fusarium Species The Plant Pathology Journal antifungal activity Bacillus amyloliquefaciens iturin A mycotoxin |
| title | Characterization of Bacillus amyloliquefaciens DA12 Showing Potent Antifungal Activity against Mycotoxigenic Fusarium Species |
| title_full | Characterization of Bacillus amyloliquefaciens DA12 Showing Potent Antifungal Activity against Mycotoxigenic Fusarium Species |
| title_fullStr | Characterization of Bacillus amyloliquefaciens DA12 Showing Potent Antifungal Activity against Mycotoxigenic Fusarium Species |
| title_full_unstemmed | Characterization of Bacillus amyloliquefaciens DA12 Showing Potent Antifungal Activity against Mycotoxigenic Fusarium Species |
| title_short | Characterization of Bacillus amyloliquefaciens DA12 Showing Potent Antifungal Activity against Mycotoxigenic Fusarium Species |
| title_sort | characterization of bacillus amyloliquefaciens da12 showing potent antifungal activity against mycotoxigenic fusarium species |
| topic | antifungal activity Bacillus amyloliquefaciens iturin A mycotoxin |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624492 |
| work_keys_str_mv | AT theresalee characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT damipark characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT kihyunkim characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT seongmilim characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT nanheeyu characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT sosookim characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT hwangyongkim characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT kyuseokjung characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT jayeongjang characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT jongchulpark characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT hyeonheuiham characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT soohyunglee characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT sungkeehong characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies AT jincheolkim characterizationofbacillusamyloliquefaciensda12showingpotentantifungalactivityagainstmycotoxigenicfusariumspecies |