Construction of a high-density linkage map and detection of sex-specific markers in Penaeus japonicus
Penaeus japonicus is one of the most important farmed shrimp species in many countries. Sexual dimorphism is observed in P. japonicus, in which females grow faster and larger than males; therefore, a unisexual female culture of P. japonicus could improve the efficiency of productivity. However, the...
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PeerJ Inc.
2021-10-01
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| Online Access: | https://peerj.com/articles/12390.pdf |
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| author | Yaqun Zhang Chuantao Zhang Na Yao Jingxian Huang Xiangshan Sun Bingran Zhao Hengde Li |
| author_facet | Yaqun Zhang Chuantao Zhang Na Yao Jingxian Huang Xiangshan Sun Bingran Zhao Hengde Li |
| author_sort | Yaqun Zhang |
| collection | DOAJ |
| description | Penaeus japonicus is one of the most important farmed shrimp species in many countries. Sexual dimorphism is observed in P. japonicus, in which females grow faster and larger than males; therefore, a unisexual female culture of P. japonicus could improve the efficiency of productivity. However, the genetic mechanisms underlying sex determination in P. japonicus are unclear. In this study, we constructed a high-density genetic linkage map of P. japonicus using genotyping-by-sequencing (GBS) technology in a full-sib family. The final map was 3,481.98 cM in length and contained 29,757 single nucleotide polymorphisms (SNPs). These SNPs were distributed on 41 sex-averaged linkage groups, with an average inter-marker distance of 0.123 cM. One haplotype, harboring five sex-specific SNPs, was detected in linkage group 1 (LG1), and its corresponding confidence interval ranged from 211.840 to 212.592 cM. Therefore, this high-density genetic linkage map will be informative for genome assembly and marker-assisted breeding, and the sex-linked SNPs will be helpful for further studies on molecular mechanisms of sex determination and unisexual culture of P. japonicus in the future. |
| first_indexed | 2024-03-09T06:23:19Z |
| format | Article |
| id | doaj.art-f10d589d029346be9bf2074f307921ed |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:23:19Z |
| publishDate | 2021-10-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-f10d589d029346be9bf2074f307921ed2023-12-03T11:33:21ZengPeerJ Inc.PeerJ2167-83592021-10-019e1239010.7717/peerj.12390Construction of a high-density linkage map and detection of sex-specific markers in Penaeus japonicusYaqun Zhang0Chuantao Zhang1Na Yao2Jingxian Huang3Xiangshan Sun4Bingran Zhao5Hengde Li6Chinese Academy of Fishery Sciences, Beijing, ChinaXiaying Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Weifang, Shandong, ChinaChinese Academy of Fishery Sciences, Beijing, ChinaXiaying Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Weifang, Shandong, ChinaXiaying Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Weifang, Shandong, ChinaXiaying Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Weifang, Shandong, ChinaChinese Academy of Fishery Sciences, Beijing, ChinaPenaeus japonicus is one of the most important farmed shrimp species in many countries. Sexual dimorphism is observed in P. japonicus, in which females grow faster and larger than males; therefore, a unisexual female culture of P. japonicus could improve the efficiency of productivity. However, the genetic mechanisms underlying sex determination in P. japonicus are unclear. In this study, we constructed a high-density genetic linkage map of P. japonicus using genotyping-by-sequencing (GBS) technology in a full-sib family. The final map was 3,481.98 cM in length and contained 29,757 single nucleotide polymorphisms (SNPs). These SNPs were distributed on 41 sex-averaged linkage groups, with an average inter-marker distance of 0.123 cM. One haplotype, harboring five sex-specific SNPs, was detected in linkage group 1 (LG1), and its corresponding confidence interval ranged from 211.840 to 212.592 cM. Therefore, this high-density genetic linkage map will be informative for genome assembly and marker-assisted breeding, and the sex-linked SNPs will be helpful for further studies on molecular mechanisms of sex determination and unisexual culture of P. japonicus in the future.https://peerj.com/articles/12390.pdfLinkage mapSex-specific markersPenaeus japonicusGenotyping-by-sequencing |
| spellingShingle | Yaqun Zhang Chuantao Zhang Na Yao Jingxian Huang Xiangshan Sun Bingran Zhao Hengde Li Construction of a high-density linkage map and detection of sex-specific markers in Penaeus japonicus PeerJ Linkage map Sex-specific markers Penaeus japonicus Genotyping-by-sequencing |
| title | Construction of a high-density linkage map and detection of sex-specific markers in Penaeus japonicus |
| title_full | Construction of a high-density linkage map and detection of sex-specific markers in Penaeus japonicus |
| title_fullStr | Construction of a high-density linkage map and detection of sex-specific markers in Penaeus japonicus |
| title_full_unstemmed | Construction of a high-density linkage map and detection of sex-specific markers in Penaeus japonicus |
| title_short | Construction of a high-density linkage map and detection of sex-specific markers in Penaeus japonicus |
| title_sort | construction of a high density linkage map and detection of sex specific markers in penaeus japonicus |
| topic | Linkage map Sex-specific markers Penaeus japonicus Genotyping-by-sequencing |
| url | https://peerj.com/articles/12390.pdf |
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