Hepatoma-derived growth factor-related protein-3 is a novel angiogenic factor.
Hepatoma-derived growth factor-related protein-3 (Hdgfrp3 or HRP-3) was recently reported as a neurotrophic factor and is upregulated in hepatocellular carcinoma to promote cancer cell survival. Here we identified HRP-3 as a new endothelial ligand and characterized its in vitro and in vivo functiona...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4440747?pdf=render |
| _version_ | 1829101591821549568 |
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| author | Michelle E LeBlanc Weiwen Wang Nora B Caberoy Xiuping Chen Feiye Guo Gabriela Alvarado Chen Shen Feng Wang Hui Wang Rui Chen Zhao-Jun Liu Keith Webster Wei Li |
| author_facet | Michelle E LeBlanc Weiwen Wang Nora B Caberoy Xiuping Chen Feiye Guo Gabriela Alvarado Chen Shen Feng Wang Hui Wang Rui Chen Zhao-Jun Liu Keith Webster Wei Li |
| author_sort | Michelle E LeBlanc |
| collection | DOAJ |
| description | Hepatoma-derived growth factor-related protein-3 (Hdgfrp3 or HRP-3) was recently reported as a neurotrophic factor and is upregulated in hepatocellular carcinoma to promote cancer cell survival. Here we identified HRP-3 as a new endothelial ligand and characterized its in vitro and in vivo functional roles and molecular signaling. We combined open reading frame phage display with multi-round in vivo binding selection to enrich retinal endothelial ligands, which were systematically identified by next generation DNA sequencing. One of the identified endothelial ligands was HRP-3. HRP-3 expression in the retina and brain was characterized by Western blot and immunohistochemistry. Cell proliferation assay showed that HRP-3 stimulated the growth of human umbilical vein endothelial cells (HUVECs). HRP-3 induced tube formation of HUVECs in culture. Wound healing assay indicated that HRP-3 promoted endothelial cell migration. HRP-3 was further confirmed for its in vitro angiogenic activity by spheroid sprouting assay. HRP-3 extrinsically activated the extracellular-signal-regulated kinase ½ (ERK1/2) pathway in endothelial cells. The angiogenic activity of HRP-3 was independently verified by mouse cornea pocket assay. Furthermore, in vivo Matrigel plug assay corroborated HRP-3 activity to promote new blood vessel formation. These results demonstrated that HRP-3 is a novel angiogenic factor. |
| first_indexed | 2024-12-10T22:48:10Z |
| format | Article |
| id | doaj.art-f10d966bddd14715910ec9504654a742 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T22:48:10Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f10d966bddd14715910ec9504654a7422022-12-22T01:30:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012790410.1371/journal.pone.0127904Hepatoma-derived growth factor-related protein-3 is a novel angiogenic factor.Michelle E LeBlancWeiwen WangNora B CaberoyXiuping ChenFeiye GuoGabriela AlvaradoChen ShenFeng WangHui WangRui ChenZhao-Jun LiuKeith WebsterWei LiHepatoma-derived growth factor-related protein-3 (Hdgfrp3 or HRP-3) was recently reported as a neurotrophic factor and is upregulated in hepatocellular carcinoma to promote cancer cell survival. Here we identified HRP-3 as a new endothelial ligand and characterized its in vitro and in vivo functional roles and molecular signaling. We combined open reading frame phage display with multi-round in vivo binding selection to enrich retinal endothelial ligands, which were systematically identified by next generation DNA sequencing. One of the identified endothelial ligands was HRP-3. HRP-3 expression in the retina and brain was characterized by Western blot and immunohistochemistry. Cell proliferation assay showed that HRP-3 stimulated the growth of human umbilical vein endothelial cells (HUVECs). HRP-3 induced tube formation of HUVECs in culture. Wound healing assay indicated that HRP-3 promoted endothelial cell migration. HRP-3 was further confirmed for its in vitro angiogenic activity by spheroid sprouting assay. HRP-3 extrinsically activated the extracellular-signal-regulated kinase ½ (ERK1/2) pathway in endothelial cells. The angiogenic activity of HRP-3 was independently verified by mouse cornea pocket assay. Furthermore, in vivo Matrigel plug assay corroborated HRP-3 activity to promote new blood vessel formation. These results demonstrated that HRP-3 is a novel angiogenic factor.http://europepmc.org/articles/PMC4440747?pdf=render |
| spellingShingle | Michelle E LeBlanc Weiwen Wang Nora B Caberoy Xiuping Chen Feiye Guo Gabriela Alvarado Chen Shen Feng Wang Hui Wang Rui Chen Zhao-Jun Liu Keith Webster Wei Li Hepatoma-derived growth factor-related protein-3 is a novel angiogenic factor. PLoS ONE |
| title | Hepatoma-derived growth factor-related protein-3 is a novel angiogenic factor. |
| title_full | Hepatoma-derived growth factor-related protein-3 is a novel angiogenic factor. |
| title_fullStr | Hepatoma-derived growth factor-related protein-3 is a novel angiogenic factor. |
| title_full_unstemmed | Hepatoma-derived growth factor-related protein-3 is a novel angiogenic factor. |
| title_short | Hepatoma-derived growth factor-related protein-3 is a novel angiogenic factor. |
| title_sort | hepatoma derived growth factor related protein 3 is a novel angiogenic factor |
| url | http://europepmc.org/articles/PMC4440747?pdf=render |
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