A Stable-Isotope Mass Spectrometry-Based Metabolic Footprinting Approach to Analyze Exudates from Phytoplankton
Phytoplankton exudates play an important role in pelagic ecology and biogeochemical cycles of elements. Exuded compounds fuel the microbial food web and often encompass bioactive secondary metabolites like sex pheromones, allelochemicals, antibiotics, or feeding attractants that mediate biological i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-10-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/11/11/4158 |
| _version_ | 1798039824896098304 |
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| author | Mark R. Viant Ulf Sommer Ralf J. M. Weber Erik Selander |
| author_facet | Mark R. Viant Ulf Sommer Ralf J. M. Weber Erik Selander |
| author_sort | Mark R. Viant |
| collection | DOAJ |
| description | Phytoplankton exudates play an important role in pelagic ecology and biogeochemical cycles of elements. Exuded compounds fuel the microbial food web and often encompass bioactive secondary metabolites like sex pheromones, allelochemicals, antibiotics, or feeding attractants that mediate biological interactions. Despite this importance, little is known about the bioactive compounds present in phytoplankton exudates. We report a stable-isotope metabolic footprinting method to characterise exudates from aquatic autotrophs. Exudates from 13C-enriched alga were concentrated by solid phase extraction and analysed by high-resolution Fourier transform ion cyclotron resonance mass spectrometry. We used the harmful algal bloom forming dinoflagellate Alexandrium tamarense to prove the method. An algorithm was developed to automatically pinpoint just those metabolites with highly 13C-enriched isotope signatures, allowing us to discover algal exudates from the complex seawater background. The stable-isotope pattern (SIP) of the detected metabolites then allowed for more accurate assignment to an empirical formula, a critical first step in their identification. This automated workflow provides an effective way to explore the chemical nature of the solutes exuded from phytoplankton cells and will facilitate the discovery of novel dissolved bioactive compounds. |
| first_indexed | 2024-04-11T21:59:01Z |
| format | Article |
| id | doaj.art-f10f95b1910a47e3a0bceb03e29f42de |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T21:59:01Z |
| publishDate | 2013-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-f10f95b1910a47e3a0bceb03e29f42de2022-12-22T04:01:00ZengMDPI AGMarine Drugs1660-33972013-10-0111114158417510.3390/md11114158A Stable-Isotope Mass Spectrometry-Based Metabolic Footprinting Approach to Analyze Exudates from PhytoplanktonMark R. ViantUlf SommerRalf J. M. WeberErik SelanderPhytoplankton exudates play an important role in pelagic ecology and biogeochemical cycles of elements. Exuded compounds fuel the microbial food web and often encompass bioactive secondary metabolites like sex pheromones, allelochemicals, antibiotics, or feeding attractants that mediate biological interactions. Despite this importance, little is known about the bioactive compounds present in phytoplankton exudates. We report a stable-isotope metabolic footprinting method to characterise exudates from aquatic autotrophs. Exudates from 13C-enriched alga were concentrated by solid phase extraction and analysed by high-resolution Fourier transform ion cyclotron resonance mass spectrometry. We used the harmful algal bloom forming dinoflagellate Alexandrium tamarense to prove the method. An algorithm was developed to automatically pinpoint just those metabolites with highly 13C-enriched isotope signatures, allowing us to discover algal exudates from the complex seawater background. The stable-isotope pattern (SIP) of the detected metabolites then allowed for more accurate assignment to an empirical formula, a critical first step in their identification. This automated workflow provides an effective way to explore the chemical nature of the solutes exuded from phytoplankton cells and will facilitate the discovery of novel dissolved bioactive compounds.http://www.mdpi.com/1660-3397/11/11/4158metabolomicsstable isotopealgal exudatedinoflagellatecorrelation analysisDIMSexometabolomeFT-ICR13Cchemical ecology |
| spellingShingle | Mark R. Viant Ulf Sommer Ralf J. M. Weber Erik Selander A Stable-Isotope Mass Spectrometry-Based Metabolic Footprinting Approach to Analyze Exudates from Phytoplankton Marine Drugs metabolomics stable isotope algal exudate dinoflagellate correlation analysis DIMS exometabolome FT-ICR 13C chemical ecology |
| title | A Stable-Isotope Mass Spectrometry-Based Metabolic Footprinting Approach to Analyze Exudates from Phytoplankton |
| title_full | A Stable-Isotope Mass Spectrometry-Based Metabolic Footprinting Approach to Analyze Exudates from Phytoplankton |
| title_fullStr | A Stable-Isotope Mass Spectrometry-Based Metabolic Footprinting Approach to Analyze Exudates from Phytoplankton |
| title_full_unstemmed | A Stable-Isotope Mass Spectrometry-Based Metabolic Footprinting Approach to Analyze Exudates from Phytoplankton |
| title_short | A Stable-Isotope Mass Spectrometry-Based Metabolic Footprinting Approach to Analyze Exudates from Phytoplankton |
| title_sort | stable isotope mass spectrometry based metabolic footprinting approach to analyze exudates from phytoplankton |
| topic | metabolomics stable isotope algal exudate dinoflagellate correlation analysis DIMS exometabolome FT-ICR 13C chemical ecology |
| url | http://www.mdpi.com/1660-3397/11/11/4158 |
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