Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/<i>β</i>-Catenin Pathway
This study explored the molecular mechanism of the plasma activation medium (PAM) inhibiting the migration ability of NSCLC (non-small cell lung cancer) cells. The effect of PAM incubation on the cell viability of NSCLC was detected through a cell viability experiment. Transwell cells and microfluid...
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MDPI AG
2023-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/7/1073 |
| _version_ | 1797590135277092864 |
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| author | Yan Zhang Zhuna Yan Hui Wu Xiao Yang Ke Yang Wencheng Song |
| author_facet | Yan Zhang Zhuna Yan Hui Wu Xiao Yang Ke Yang Wencheng Song |
| author_sort | Yan Zhang |
| collection | DOAJ |
| description | This study explored the molecular mechanism of the plasma activation medium (PAM) inhibiting the migration ability of NSCLC (non-small cell lung cancer) cells. The effect of PAM incubation on the cell viability of NSCLC was detected through a cell viability experiment. Transwell cells and microfluidic chips were used to investigate the effects of PAM on the migration capacity of NSCLC cells, and the latter was used for the first time to observe the changes in the migration capacity of cancer cells treated with PAM. Moreover, the molecular mechanisms of PAM affecting the migration ability of NSCLC cells were investigated through intracellular and extracellular ROS detection, mitochondrial membrane potential, and Western blot experiments. The results showed that after long-term treatment with PAM, the high level of ROS produced by PAM reduced the level of the mitochondrial membrane potential of cells and blocked the cell division cycle in the G2/M phase. At the same time, the EMT process was reversed by inhibiting the Wnt/<i>β</i>-catenin signaling pathway. These results suggested that the high ROS levels generated by the PAM treatment reversed the EMT process by inhibiting the WNT/<i>β</i>-catenin pathway in NSCLC cells and thus inhibited the migration of NSCLC cells. Therefore, these results provide good theoretical support for the clinical treatment of NSCLC with PAM. |
| first_indexed | 2024-03-11T01:16:07Z |
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| id | doaj.art-f110b18af23e423e8eca8dfd2ce40af1 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T01:16:07Z |
| publishDate | 2023-07-01 |
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| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-f110b18af23e423e8eca8dfd2ce40af12023-11-18T18:31:02ZengMDPI AGBiomolecules2218-273X2023-07-01137107310.3390/biom13071073Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/<i>β</i>-Catenin PathwayYan Zhang0Zhuna Yan1Hui Wu2Xiao Yang3Ke Yang4Wencheng Song5School of Medicine, Anhui University of Science and Technology, Huainan 232001, ChinaSchool of Medicine, Anhui University of Science and Technology, Huainan 232001, ChinaAnhui Institute of Optics and Fine Mechanics, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaAnhui Institute of Optics and Fine Mechanics, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaAnhui Institute of Optics and Fine Mechanics, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaSchool of Medicine, Anhui University of Science and Technology, Huainan 232001, ChinaThis study explored the molecular mechanism of the plasma activation medium (PAM) inhibiting the migration ability of NSCLC (non-small cell lung cancer) cells. The effect of PAM incubation on the cell viability of NSCLC was detected through a cell viability experiment. Transwell cells and microfluidic chips were used to investigate the effects of PAM on the migration capacity of NSCLC cells, and the latter was used for the first time to observe the changes in the migration capacity of cancer cells treated with PAM. Moreover, the molecular mechanisms of PAM affecting the migration ability of NSCLC cells were investigated through intracellular and extracellular ROS detection, mitochondrial membrane potential, and Western blot experiments. The results showed that after long-term treatment with PAM, the high level of ROS produced by PAM reduced the level of the mitochondrial membrane potential of cells and blocked the cell division cycle in the G2/M phase. At the same time, the EMT process was reversed by inhibiting the Wnt/<i>β</i>-catenin signaling pathway. These results suggested that the high ROS levels generated by the PAM treatment reversed the EMT process by inhibiting the WNT/<i>β</i>-catenin pathway in NSCLC cells and thus inhibited the migration of NSCLC cells. Therefore, these results provide good theoretical support for the clinical treatment of NSCLC with PAM.https://www.mdpi.com/2218-273X/13/7/1073plasma-activated mediumNSCLCmigrationWnt/<i>β</i>-cateninRONS |
| spellingShingle | Yan Zhang Zhuna Yan Hui Wu Xiao Yang Ke Yang Wencheng Song Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/<i>β</i>-Catenin Pathway Biomolecules plasma-activated medium NSCLC migration Wnt/<i>β</i>-catenin RONS |
| title | Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/<i>β</i>-Catenin Pathway |
| title_full | Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/<i>β</i>-Catenin Pathway |
| title_fullStr | Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/<i>β</i>-Catenin Pathway |
| title_full_unstemmed | Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/<i>β</i>-Catenin Pathway |
| title_short | Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/<i>β</i>-Catenin Pathway |
| title_sort | low temperature plasma activated medium inhibits the migration of non small cell lung cancer cells via the wnt i β i catenin pathway |
| topic | plasma-activated medium NSCLC migration Wnt/<i>β</i>-catenin RONS |
| url | https://www.mdpi.com/2218-273X/13/7/1073 |
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