EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG
Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universitas Lampung
2017-04-01
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| Series: | Jurnal Hama dan Penyakit Tumbuhan Tropika |
| Subjects: | |
| Online Access: | http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/355 |
| _version_ | 1819130615964893184 |
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| author | Johanna Taribuka Christanti Sumardiyono Siti Muslimah Widyastuti Arif Wibowo |
| author_facet | Johanna Taribuka Christanti Sumardiyono Siti Muslimah Widyastuti Arif Wibowo |
| author_sort | Johanna Taribuka |
| collection | DOAJ |
| description | Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%. |
| first_indexed | 2024-12-22T09:02:26Z |
| format | Article |
| id | doaj.art-f116568090a443f38b43286235b999b6 |
| institution | Directory Open Access Journal |
| issn | 1411-7525 2461-0399 |
| language | English |
| last_indexed | 2024-12-22T09:02:26Z |
| publishDate | 2017-04-01 |
| publisher | Universitas Lampung |
| record_format | Article |
| series | Jurnal Hama dan Penyakit Tumbuhan Tropika |
| spelling | doaj.art-f116568090a443f38b43286235b999b62022-12-21T18:31:42ZengUniversitas LampungJurnal Hama dan Penyakit Tumbuhan Tropika1411-75252461-03992017-04-0116211512310.23960/j.hptt.216115-123319EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANGJohanna TaribukaChristanti SumardiyonoSiti Muslimah WidyastutiArif WibowoExploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/355IdentificationbananaFusarium oxysporum f.sp. cubenseTrichoderma endophytic |
| spellingShingle | Johanna Taribuka Christanti Sumardiyono Siti Muslimah Widyastuti Arif Wibowo EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Jurnal Hama dan Penyakit Tumbuhan Tropika Identification banana Fusarium oxysporum f.sp. cubense Trichoderma endophytic |
| title | EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG |
| title_full | EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG |
| title_fullStr | EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG |
| title_full_unstemmed | EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG |
| title_short | EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG |
| title_sort | eksplorasi dan identifikasi trichoderma endofitik pada pisang |
| topic | Identification banana Fusarium oxysporum f.sp. cubense Trichoderma endophytic |
| url | http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/355 |
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