Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress
Salinity is one of the major abiotic stress that limits crop growth and productivity. We investigated the transcriptomes of salt-treated soybean seedlings versus a control using RNA-seq to better understand the molecular mechanisms of the soybean (Glycine max L.) response to salt stress. Transcripto...
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PeerJ Inc.
2021-11-01
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| Online Access: | https://peerj.com/articles/12492.pdf |
| _version_ | 1797418355120930816 |
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| author | Xun Liu Xinxia Yang Bin Zhang |
| author_facet | Xun Liu Xinxia Yang Bin Zhang |
| author_sort | Xun Liu |
| collection | DOAJ |
| description | Salinity is one of the major abiotic stress that limits crop growth and productivity. We investigated the transcriptomes of salt-treated soybean seedlings versus a control using RNA-seq to better understand the molecular mechanisms of the soybean (Glycine max L.) response to salt stress. Transcriptome analysis revealed 1,235 differentially expressed genes (DEGs) under salt stress. Several important pathways and key candidate genes were identified by KEGG enrichment. A total of 116 differentially expressed transcription factors (TFs) were identified, and 17 TFs were found to belong to MYB families. Phylogenetic analysis revealed that these TFs may be involved in salt stress adaptation. Further analysis revealed that GmMYB46 was up-regulated by salt and mannitol and was localized in the nucleus. The salt tolerance of transgenic Arabidopsis overexpressing GmMYB46 was significantly enhanced compared to wild-type (WT). GmMYB46 activates the expression of salt stress response genes (P5CS1, SOD, POD, NCED3) in Arabidopsis under salt stress, indicating that the GmMYB46 protein mediates the salt stress response through complex regulatory mechanisms. This study provides information with which to better understand the molecular mechanism of salt tolerance in soybeans and to genetically improve the crop. |
| first_indexed | 2024-03-09T06:31:36Z |
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| language | English |
| last_indexed | 2024-03-09T06:31:36Z |
| publishDate | 2021-11-01 |
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| spelling | doaj.art-f116736375fc4e5fb52502adfe1b35ce2023-12-03T11:05:35ZengPeerJ Inc.PeerJ2167-83592021-11-019e1249210.7717/peerj.12492Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stressXun Liu0Xinxia Yang1Bin Zhang2College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou, ChinaDepartment of Logistics, Hunan University of Science and Engineering, Yongzhou, ChinaCollege of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou, ChinaSalinity is one of the major abiotic stress that limits crop growth and productivity. We investigated the transcriptomes of salt-treated soybean seedlings versus a control using RNA-seq to better understand the molecular mechanisms of the soybean (Glycine max L.) response to salt stress. Transcriptome analysis revealed 1,235 differentially expressed genes (DEGs) under salt stress. Several important pathways and key candidate genes were identified by KEGG enrichment. A total of 116 differentially expressed transcription factors (TFs) were identified, and 17 TFs were found to belong to MYB families. Phylogenetic analysis revealed that these TFs may be involved in salt stress adaptation. Further analysis revealed that GmMYB46 was up-regulated by salt and mannitol and was localized in the nucleus. The salt tolerance of transgenic Arabidopsis overexpressing GmMYB46 was significantly enhanced compared to wild-type (WT). GmMYB46 activates the expression of salt stress response genes (P5CS1, SOD, POD, NCED3) in Arabidopsis under salt stress, indicating that the GmMYB46 protein mediates the salt stress response through complex regulatory mechanisms. This study provides information with which to better understand the molecular mechanism of salt tolerance in soybeans and to genetically improve the crop.https://peerj.com/articles/12492.pdfSoybeanTranscriptome analysisSalt stressDifferentially expressed genesGmMYB46 |
| spellingShingle | Xun Liu Xinxia Yang Bin Zhang Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress PeerJ Soybean Transcriptome analysis Salt stress Differentially expressed genes GmMYB46 |
| title | Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress |
| title_full | Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress |
| title_fullStr | Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress |
| title_full_unstemmed | Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress |
| title_short | Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress |
| title_sort | transcriptome analysis and functional identification of gmmyb46 in soybean seedlings under salt stress |
| topic | Soybean Transcriptome analysis Salt stress Differentially expressed genes GmMYB46 |
| url | https://peerj.com/articles/12492.pdf |
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