Bacterial colonization of seromas after breast cancer surgery with and without local steroid prophylaxis
Abstract Background Seroma formation is a frequent postoperative sequela after mastectomy for primary breast cancer. We investigated the role of bacterial colonization of seroma fluid with three different culture methods and the effect of intracavitary steroids. Methods The study group consisted of...
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | World Journal of Surgical Oncology |
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| Online Access: | http://link.springer.com/article/10.1186/s12957-019-1661-1 |
| _version_ | 1818826147500130304 |
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| author | Christen Kirk Axelsson Gro Maria Qvamme Mette Okholm Charlotte Lanng Magnus Arpi Minea Bruusgaard Mortensen Birgitte Wegeberg Pal Bela Szecsi |
| author_facet | Christen Kirk Axelsson Gro Maria Qvamme Mette Okholm Charlotte Lanng Magnus Arpi Minea Bruusgaard Mortensen Birgitte Wegeberg Pal Bela Szecsi |
| author_sort | Christen Kirk Axelsson |
| collection | DOAJ |
| description | Abstract Background Seroma formation is a frequent postoperative sequela after mastectomy for primary breast cancer. We investigated the role of bacterial colonization of seroma fluid with three different culture methods and the effect of intracavitary steroids. Methods The study group consisted of 212 patients scheduled for mastectomy from a previously performed double-blind randomized placebo-controlled intervention trial. The patients were allocated to a single dose of 80 mg of steroids (methylprednisolone) or saline, and the effect on seroma formation was investigated. From each aspiration, an equal volume of seroma fluid (10 mL) was distributed into one sterile transport tube (conventional method), one aerobic blood culture bottle and one anaerobic blood culture bottle. Results There was significant variation in the number of bacterial species detected in seroma samples among the three culture methods, ranging from 18 species with the conventional culture tubes to 40 species with aerobic blood culture bottles. Patients receiving prophylactic steroids had significantly more frequent colonization than those in the saline group. Nevertheless, the clinical surgical site infection rate of 7.0% was equal between the two groups. Conclusions In general, data analysis of the entire set of case material did not succeed in demonstrating a relationship between a specific bacterial species or a combination of species and seroma formation. However, in the few patients with growth of a pathogenic species, both the duration of seroma formation and volume of seroma fluid were more pronounced. Trial registration: Ethics Committee of Copenhagen (H-4-2009-137), (EudraCT number 2009-016650-40), the Danish Data Protection Agency (code J. no. F.750.75-2), and the Danish Health and Medicines Authority (sponsor protocol code number 23837). Start date November 2010. |
| first_indexed | 2024-12-19T00:23:02Z |
| format | Article |
| id | doaj.art-f117c34d064745e6b5e806895ea0c1a6 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-12-19T00:23:02Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-f117c34d064745e6b5e806895ea0c1a62022-12-21T20:45:28ZengBMCWorld Journal of Surgical Oncology1477-78192019-07-011711710.1186/s12957-019-1661-1Bacterial colonization of seromas after breast cancer surgery with and without local steroid prophylaxisChristen Kirk Axelsson0Gro Maria Qvamme1Mette Okholm2Charlotte Lanng3Magnus Arpi4Minea Bruusgaard Mortensen5Birgitte Wegeberg6Pal Bela Szecsi7Department of Breast Surgery F, University of Copenhagen, Herlev HospitalDepartment of Breast Surgery F, University of Copenhagen, Herlev HospitalDepartment of Breast Surgery F, University of Copenhagen, Herlev HospitalDepartment of Breast Surgery F, University of Copenhagen, Herlev HospitalDepartment of Clinical Microbiology, University of Copenhagen, Herlev HospitalDepartment of Breast Surgery F, University of Copenhagen, Herlev HospitalDepartment of Breast Surgery F, University of Copenhagen, Herlev HospitalDepartment of Clinical Biochemistry, University of Copenhagen, Holbæk HospitalAbstract Background Seroma formation is a frequent postoperative sequela after mastectomy for primary breast cancer. We investigated the role of bacterial colonization of seroma fluid with three different culture methods and the effect of intracavitary steroids. Methods The study group consisted of 212 patients scheduled for mastectomy from a previously performed double-blind randomized placebo-controlled intervention trial. The patients were allocated to a single dose of 80 mg of steroids (methylprednisolone) or saline, and the effect on seroma formation was investigated. From each aspiration, an equal volume of seroma fluid (10 mL) was distributed into one sterile transport tube (conventional method), one aerobic blood culture bottle and one anaerobic blood culture bottle. Results There was significant variation in the number of bacterial species detected in seroma samples among the three culture methods, ranging from 18 species with the conventional culture tubes to 40 species with aerobic blood culture bottles. Patients receiving prophylactic steroids had significantly more frequent colonization than those in the saline group. Nevertheless, the clinical surgical site infection rate of 7.0% was equal between the two groups. Conclusions In general, data analysis of the entire set of case material did not succeed in demonstrating a relationship between a specific bacterial species or a combination of species and seroma formation. However, in the few patients with growth of a pathogenic species, both the duration of seroma formation and volume of seroma fluid were more pronounced. Trial registration: Ethics Committee of Copenhagen (H-4-2009-137), (EudraCT number 2009-016650-40), the Danish Data Protection Agency (code J. no. F.750.75-2), and the Danish Health and Medicines Authority (sponsor protocol code number 23837). Start date November 2010.http://link.springer.com/article/10.1186/s12957-019-1661-1SeromaBreast cancerBreast neoplasmSurgerySurgical wound infectionMicrobiology |
| spellingShingle | Christen Kirk Axelsson Gro Maria Qvamme Mette Okholm Charlotte Lanng Magnus Arpi Minea Bruusgaard Mortensen Birgitte Wegeberg Pal Bela Szecsi Bacterial colonization of seromas after breast cancer surgery with and without local steroid prophylaxis World Journal of Surgical Oncology Seroma Breast cancer Breast neoplasm Surgery Surgical wound infection Microbiology |
| title | Bacterial colonization of seromas after breast cancer surgery with and without local steroid prophylaxis |
| title_full | Bacterial colonization of seromas after breast cancer surgery with and without local steroid prophylaxis |
| title_fullStr | Bacterial colonization of seromas after breast cancer surgery with and without local steroid prophylaxis |
| title_full_unstemmed | Bacterial colonization of seromas after breast cancer surgery with and without local steroid prophylaxis |
| title_short | Bacterial colonization of seromas after breast cancer surgery with and without local steroid prophylaxis |
| title_sort | bacterial colonization of seromas after breast cancer surgery with and without local steroid prophylaxis |
| topic | Seroma Breast cancer Breast neoplasm Surgery Surgical wound infection Microbiology |
| url | http://link.springer.com/article/10.1186/s12957-019-1661-1 |
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