Occurrence of <i>Salmonella</i> in the Cattle Production in France
<i>Salmonella</i> is among the most common foodborne pathogens worldwide, and can lead to acute gastroenteritis. Along with poultry, cattle production is recognized as an important source of human infection. <i>Salmonella</i> transmission from cattle to humans can occur throu...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/4/872 |
| _version_ | 1797537291912085504 |
|---|---|
| author | Laetitia Bonifait Amandine Thépault Louise Baugé Sandra Rouxel Françoise Le Gall Marianne Chemaly |
| author_facet | Laetitia Bonifait Amandine Thépault Louise Baugé Sandra Rouxel Françoise Le Gall Marianne Chemaly |
| author_sort | Laetitia Bonifait |
| collection | DOAJ |
| description | <i>Salmonella</i> is among the most common foodborne pathogens worldwide, and can lead to acute gastroenteritis. Along with poultry, cattle production is recognized as an important source of human infection. <i>Salmonella</i> transmission from cattle to humans can occur through the environment, or through close contact with sick animals or their derived products. This study aimed to investigate the intestinal carriage of <i>Salmonella</i> spp. within French cattle production. A total of 959 cattle intestinal samples, from one of the largest French slaughterhouses, were analyzed. Isolated strains were genotyped by pulsed field gel electrophoresis (PFGE), and a sub-selection was taken by whole genome sequencing (WGS). Twenty-nine samples were positive for <i>Salmonella</i> spp., yielding an estimated prevalence of 3% in cattle production. Eight different <i>Salmonella</i> serotypes were found: Montevideo was the most prevalent (34%), followed by Mbandaka (24%) and Anatum (14%). PFGE genotyping allowed the clustering of <i>Salmonella</i> isolates according to their serotype. Within the clusters, some isolates presented 100% similarity. To investigate potential epidemiological links between them, WGS and core genome multilocus sequence typing (cgMLST) were used, revealing identical profiles between isolates originating from different areas and/or different animal breeds. This investigation provides new insights on <i>Salmonella</i> serotype epidemiology in cattle production in France. |
| first_indexed | 2024-03-10T12:13:59Z |
| format | Article |
| id | doaj.art-f1188cdfb0564b18ba3ea45bfcba99c6 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T12:13:59Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f1188cdfb0564b18ba3ea45bfcba99c62023-11-21T16:02:44ZengMDPI AGMicroorganisms2076-26072021-04-019487210.3390/microorganisms9040872Occurrence of <i>Salmonella</i> in the Cattle Production in FranceLaetitia Bonifait0Amandine Thépault1Louise Baugé2Sandra Rouxel3Françoise Le Gall4Marianne Chemaly5Unit of Hygiene and Quality of Poultry and Pork Products, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Laboratory of Ploufragan-Plouzané-Niort Site BP 53, 22440 Ploufragan, FranceUnit of Hygiene and Quality of Poultry and Pork Products, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Laboratory of Ploufragan-Plouzané-Niort Site BP 53, 22440 Ploufragan, FranceUnit of Hygiene and Quality of Poultry and Pork Products, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Laboratory of Ploufragan-Plouzané-Niort Site BP 53, 22440 Ploufragan, FranceUnit of Hygiene and Quality of Poultry and Pork Products, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Laboratory of Ploufragan-Plouzané-Niort Site BP 53, 22440 Ploufragan, FranceUnit of Hygiene and Quality of Poultry and Pork Products, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Laboratory of Ploufragan-Plouzané-Niort Site BP 53, 22440 Ploufragan, FranceUnit of Hygiene and Quality of Poultry and Pork Products, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Laboratory of Ploufragan-Plouzané-Niort Site BP 53, 22440 Ploufragan, France<i>Salmonella</i> is among the most common foodborne pathogens worldwide, and can lead to acute gastroenteritis. Along with poultry, cattle production is recognized as an important source of human infection. <i>Salmonella</i> transmission from cattle to humans can occur through the environment, or through close contact with sick animals or their derived products. This study aimed to investigate the intestinal carriage of <i>Salmonella</i> spp. within French cattle production. A total of 959 cattle intestinal samples, from one of the largest French slaughterhouses, were analyzed. Isolated strains were genotyped by pulsed field gel electrophoresis (PFGE), and a sub-selection was taken by whole genome sequencing (WGS). Twenty-nine samples were positive for <i>Salmonella</i> spp., yielding an estimated prevalence of 3% in cattle production. Eight different <i>Salmonella</i> serotypes were found: Montevideo was the most prevalent (34%), followed by Mbandaka (24%) and Anatum (14%). PFGE genotyping allowed the clustering of <i>Salmonella</i> isolates according to their serotype. Within the clusters, some isolates presented 100% similarity. To investigate potential epidemiological links between them, WGS and core genome multilocus sequence typing (cgMLST) were used, revealing identical profiles between isolates originating from different areas and/or different animal breeds. This investigation provides new insights on <i>Salmonella</i> serotype epidemiology in cattle production in France.https://www.mdpi.com/2076-2607/9/4/872<i>Salmonella</i>cattle productionwhole genome sequencingFrance |
| spellingShingle | Laetitia Bonifait Amandine Thépault Louise Baugé Sandra Rouxel Françoise Le Gall Marianne Chemaly Occurrence of <i>Salmonella</i> in the Cattle Production in France Microorganisms <i>Salmonella</i> cattle production whole genome sequencing France |
| title | Occurrence of <i>Salmonella</i> in the Cattle Production in France |
| title_full | Occurrence of <i>Salmonella</i> in the Cattle Production in France |
| title_fullStr | Occurrence of <i>Salmonella</i> in the Cattle Production in France |
| title_full_unstemmed | Occurrence of <i>Salmonella</i> in the Cattle Production in France |
| title_short | Occurrence of <i>Salmonella</i> in the Cattle Production in France |
| title_sort | occurrence of i salmonella i in the cattle production in france |
| topic | <i>Salmonella</i> cattle production whole genome sequencing France |
| url | https://www.mdpi.com/2076-2607/9/4/872 |
| work_keys_str_mv | AT laetitiabonifait occurrenceofisalmonellaiinthecattleproductioninfrance AT amandinethepault occurrenceofisalmonellaiinthecattleproductioninfrance AT louisebauge occurrenceofisalmonellaiinthecattleproductioninfrance AT sandrarouxel occurrenceofisalmonellaiinthecattleproductioninfrance AT francoiselegall occurrenceofisalmonellaiinthecattleproductioninfrance AT mariannechemaly occurrenceofisalmonellaiinthecattleproductioninfrance |