Association between Downstream Taste Signaling Genes, Oral Microbiome, and Severe Early Childhood Caries
Polymorphisms in taste receptor genes have been shown to play a role in early childhood caries (ECC), a multifactorial, biofilm-mediated disease. This study aimed to evaluate associations between severe-ECC (S-ECC), the oral microbiome, and variants in genes that encode components of the G protein-c...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/81 |
| _version_ | 1797625726493523968 |
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| author | Vivianne Cruz de Jesus Betty-Anne Mittermuller Pingzhao Hu Robert J. Schroth Prashen Chelikani |
| author_facet | Vivianne Cruz de Jesus Betty-Anne Mittermuller Pingzhao Hu Robert J. Schroth Prashen Chelikani |
| author_sort | Vivianne Cruz de Jesus |
| collection | DOAJ |
| description | Polymorphisms in taste receptor genes have been shown to play a role in early childhood caries (ECC), a multifactorial, biofilm-mediated disease. This study aimed to evaluate associations between severe-ECC (S-ECC), the oral microbiome, and variants in genes that encode components of the G protein-coupled receptor (GPCR) signaling cascade involved in taste sensation. A total of 176 children (88 caries-free; 88 with S-ECC) were recruited. Analyses of <i>16S</i> and <i>ITS1 rRNA</i> microbial genes and seven (<i>GNAQ, GNAS, GNAT3, GNAI2, RAC1, RALB</i>, and <i>PLCB2</i>) human genes were pursued using next-generation sequencing. Regression analyses were performed to evaluate associations between genetic variants, S-ECC, and the supragingival plaque microbiome. Results suggest that <i>PLCB2</i> rs2305645 (T), rs1869901 (G), and rs2305649 (G) alleles had a protective effect on S-ECC (rs2305645, odds ratio (OR) = 0.27 (95% confidence interval (CI): 0.14–0.51); rs1869901, OR = 0.34 (95% CI: 0.20–0.58); and rs2305649, OR = 0.43 (95% CI: 0.26–0.71)). Variants in <i>GNAQ, GNAS, GNAT3, PLCB2, RALB</i>, and <i>RAC1</i> were associated with oral fungal and bacterial community composition. This study revealed that three loci at <i>PLCB2</i> are significantly associated with S-ECC. Variants in multiple genes were associated with the composition of dental biofilm. These findings contribute to the current knowledge about the role of genetics in S-ECC. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T10:00:24Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f121249d1d624a50acdffd061e8294be2023-11-16T15:29:01ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-012418110.3390/ijms24010081Association between Downstream Taste Signaling Genes, Oral Microbiome, and Severe Early Childhood CariesVivianne Cruz de Jesus0Betty-Anne Mittermuller1Pingzhao Hu2Robert J. Schroth3Prashen Chelikani4Manitoba Chemosensory Biology Research Group, Department of Oral Biology, University of Manitoba, Winnipeg, MB R3E 0W2, CanadaManitoba Chemosensory Biology Research Group, Department of Oral Biology, University of Manitoba, Winnipeg, MB R3E 0W2, CanadaChildren’s Hospital Research Institute of Manitoba (CHRIM), Winnipeg, MB R3E 3P4, CanadaManitoba Chemosensory Biology Research Group, Department of Oral Biology, University of Manitoba, Winnipeg, MB R3E 0W2, CanadaManitoba Chemosensory Biology Research Group, Department of Oral Biology, University of Manitoba, Winnipeg, MB R3E 0W2, CanadaPolymorphisms in taste receptor genes have been shown to play a role in early childhood caries (ECC), a multifactorial, biofilm-mediated disease. This study aimed to evaluate associations between severe-ECC (S-ECC), the oral microbiome, and variants in genes that encode components of the G protein-coupled receptor (GPCR) signaling cascade involved in taste sensation. A total of 176 children (88 caries-free; 88 with S-ECC) were recruited. Analyses of <i>16S</i> and <i>ITS1 rRNA</i> microbial genes and seven (<i>GNAQ, GNAS, GNAT3, GNAI2, RAC1, RALB</i>, and <i>PLCB2</i>) human genes were pursued using next-generation sequencing. Regression analyses were performed to evaluate associations between genetic variants, S-ECC, and the supragingival plaque microbiome. Results suggest that <i>PLCB2</i> rs2305645 (T), rs1869901 (G), and rs2305649 (G) alleles had a protective effect on S-ECC (rs2305645, odds ratio (OR) = 0.27 (95% confidence interval (CI): 0.14–0.51); rs1869901, OR = 0.34 (95% CI: 0.20–0.58); and rs2305649, OR = 0.43 (95% CI: 0.26–0.71)). Variants in <i>GNAQ, GNAS, GNAT3, PLCB2, RALB</i>, and <i>RAC1</i> were associated with oral fungal and bacterial community composition. This study revealed that three loci at <i>PLCB2</i> are significantly associated with S-ECC. Variants in multiple genes were associated with the composition of dental biofilm. These findings contribute to the current knowledge about the role of genetics in S-ECC.https://www.mdpi.com/1422-0067/24/1/81dental cariestooth decaybacteriafungitaste receptors |
| spellingShingle | Vivianne Cruz de Jesus Betty-Anne Mittermuller Pingzhao Hu Robert J. Schroth Prashen Chelikani Association between Downstream Taste Signaling Genes, Oral Microbiome, and Severe Early Childhood Caries International Journal of Molecular Sciences dental caries tooth decay bacteria fungi taste receptors |
| title | Association between Downstream Taste Signaling Genes, Oral Microbiome, and Severe Early Childhood Caries |
| title_full | Association between Downstream Taste Signaling Genes, Oral Microbiome, and Severe Early Childhood Caries |
| title_fullStr | Association between Downstream Taste Signaling Genes, Oral Microbiome, and Severe Early Childhood Caries |
| title_full_unstemmed | Association between Downstream Taste Signaling Genes, Oral Microbiome, and Severe Early Childhood Caries |
| title_short | Association between Downstream Taste Signaling Genes, Oral Microbiome, and Severe Early Childhood Caries |
| title_sort | association between downstream taste signaling genes oral microbiome and severe early childhood caries |
| topic | dental caries tooth decay bacteria fungi taste receptors |
| url | https://www.mdpi.com/1422-0067/24/1/81 |
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