Development of a Melting-Curve-Based Multiplex Real-Time PCR Assay for the Simultaneous Detection of Viruses Causing Respiratory Infection

Development of a Melting-Curve-Based Multiplex Real-Time PCR Assay for the Simultaneous Detection of Viruses Causing Respiratory Infection

The prompt and accurate identification of the etiological agents of viral respiratory infections is a critical measure in mitigating outbreaks. In this study, we developed and clinically evaluated a novel melting-curve-based multiplex real-time PCR (M-m-qPCR) assay targeting the RNA-dependent RNA po...

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Bibliographic Details
Main Authors: Eliandro Reis Tavares, Thiago Ferreira de Lima, Guilherme Bartolomeu-Gonçalves, Isabela Madeira de Castro, Daniel Gaiotto de Lima, Paulo Henrique Guilherme Borges, Gerson Nakazato, Renata Katsuko Takayama Kobayashi, Emerson José Venancio, César Ricardo Teixeira Tarley, Elaine Regina Delicato de Almeida, Marsileni Pelisson, Eliana Carolina Vespero, Andrea Name Colado Simão, Márcia Regina Eches Perugini, Gilselena Kerbauy, Marco Aurélio Fornazieri, Maria Cristina Bronharo Tognim, Viviane Monteiro Góes, Tatiana de Arruda Campos Brasil de Souza, Danielle Bruna Leal Oliveira, Edison Luiz Durigon, Lígia Carla Faccin-Galhardi, Lucy Megumi Yamauchi, Sueli Fumie Yamada-Ogatta
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:Microorganisms
Subjects:
COVID-19
SARS-CoV-2
Influenza A virus
Human Respiratory Syncytial Virus
Human Rhinovirus B
nucleic acid amplification test
Online Access:https://www.mdpi.com/2076-2607/11/11/2692

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