Construction of a Three-Color Prism-Based TIRF Microscope to Study the Interactions and Dynamics of Macromolecules

Construction of a Three-Color Prism-Based TIRF Microscope to Study the Interactions and Dynamics of Macromolecules

Single-molecule total internal reflection fluorescence (TIRF) microscopy allows for the real-time visualization of macromolecular dynamics and complex assembly. Prism-based TIRF microscopes (prismTIRF) are relatively simple to operate and can be easily modulated to fit the needs of a wide variety of...

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Bibliographic Details
Main Authors: Max S. Fairlamb, Amy M. Whitaker, Fletcher E. Bain, Maria Spies, Bret D. Freudenthal
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Biology
Subjects:
single-molecule TIRF
prism
single-molecule FRET
smFRET
construction guide
protein–DNA complexes
Online Access:https://www.mdpi.com/2079-7737/10/7/571

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