Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from Dromedary
Secretory group V phospholipase A2 (PLA<sub>2</sub>-V) is known to be involved in inflammatory processes in cellular studies, nevertheless, the biochemical and the enzymatic characteristics of this important enzyme have been unclear yet. We reported, as a first step towards understanding...
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MDPI AG
2022-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/11/3437 |
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| author | Mona Alonazi Aida Karray Raida Jallouli Abir Ben Bacha |
| author_facet | Mona Alonazi Aida Karray Raida Jallouli Abir Ben Bacha |
| author_sort | Mona Alonazi |
| collection | DOAJ |
| description | Secretory group V phospholipase A2 (PLA<sub>2</sub>-V) is known to be involved in inflammatory processes in cellular studies, nevertheless, the biochemical and the enzymatic characteristics of this important enzyme have been unclear yet. We reported, as a first step towards understanding the biochemical properties, catalytic characteristics, antimicrobial and cytotoxic effects of this PLA<sub>2</sub>, the production of PLA<sub>2</sub>-V from dromedary. The obtained DrPLA<sub>2</sub>-V has an absolute requirement for Ca<sup>2+</sup> and NaTDC for enzymatic activity with an optimum pH of 9 and temperature of 45 °C with phosphatidylethanolamine as a substrate. Kinetic parameters showed that <i>K<sub>cat</sub></i>/<i>Km</i><sub>app</sub> is 2.6 ± 0.02 mM<sup>−1</sup> s<sup>−1</sup>. The enzyme was found to display potent Gram-positive bactericidal activity (with IC50 values of about 5 µg/mL) and antifungal activity (with IC50 values of about 25 µg/mL)in vitro. However, the purified enzyme did not display a cytotoxic effect against cancer cells. |
| first_indexed | 2024-03-10T01:04:59Z |
| format | Article |
| id | doaj.art-f1244c510c0e4dcd9e3dfa9b7e806a97 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:04:59Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f1244c510c0e4dcd9e3dfa9b7e806a972023-11-23T14:28:49ZengMDPI AGMolecules1420-30492022-05-012711343710.3390/molecules27113437Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from DromedaryMona Alonazi0Aida Karray1Raida Jallouli2Abir Ben Bacha3Biochemistry Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaLaboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS Route de Soukra, Université de Sfax-Tunisia, Sfax 3038, TunisiaInstitut de Pharmacologie de Sherbrooke, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, CanadaBiochemistry Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaSecretory group V phospholipase A2 (PLA<sub>2</sub>-V) is known to be involved in inflammatory processes in cellular studies, nevertheless, the biochemical and the enzymatic characteristics of this important enzyme have been unclear yet. We reported, as a first step towards understanding the biochemical properties, catalytic characteristics, antimicrobial and cytotoxic effects of this PLA<sub>2</sub>, the production of PLA<sub>2</sub>-V from dromedary. The obtained DrPLA<sub>2</sub>-V has an absolute requirement for Ca<sup>2+</sup> and NaTDC for enzymatic activity with an optimum pH of 9 and temperature of 45 °C with phosphatidylethanolamine as a substrate. Kinetic parameters showed that <i>K<sub>cat</sub></i>/<i>Km</i><sub>app</sub> is 2.6 ± 0.02 mM<sup>−1</sup> s<sup>−1</sup>. The enzyme was found to display potent Gram-positive bactericidal activity (with IC50 values of about 5 µg/mL) and antifungal activity (with IC50 values of about 25 µg/mL)in vitro. However, the purified enzyme did not display a cytotoxic effect against cancer cells.https://www.mdpi.com/1420-3049/27/11/3437phospholipase Vkineticscharacterizationbiological activities |
| spellingShingle | Mona Alonazi Aida Karray Raida Jallouli Abir Ben Bacha Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from Dromedary Molecules phospholipase V kinetics characterization biological activities |
| title | Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from Dromedary |
| title_full | Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from Dromedary |
| title_fullStr | Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from Dromedary |
| title_full_unstemmed | Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from Dromedary |
| title_short | Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from Dromedary |
| title_sort | biochemical kinetic and biological properties of group v phospholipase a2 from dromedary |
| topic | phospholipase V kinetics characterization biological activities |
| url | https://www.mdpi.com/1420-3049/27/11/3437 |
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