Beclin-1 improves the cognitive function of mice with alzheimer"s disease
We aimed to investigate the improvement effect of Beclin-1 on the cognitive function of mice with Alzheimer"s disease (AD). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were isolated from neonatal umbilical cord tissues. The cells stably overexpressing Beclin-1 were constructe...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2023-12-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3061 |
| _version_ | 1827382658184773632 |
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| author | Li SHAO Qing HE Xin DU Qing LI Siyuan YANG Chen DONG Pengju MA |
| author_facet | Li SHAO Qing HE Xin DU Qing LI Siyuan YANG Chen DONG Pengju MA |
| author_sort | Li SHAO |
| collection | DOAJ |
| description | We aimed to investigate the improvement effect of Beclin-1 on the cognitive function
of mice with Alzheimer"s disease (AD). Human umbilical cord-derived mesenchymal
stem cells (hUC-MSCs) were isolated from neonatal umbilical cord tissues. The cells
stably overexpressing Beclin-1 were constructed, and those of passages 5, 10, and 15
were used. Forty-eight AD mice were randomly divided into AD, P5 hUC-MSCs, P15
hUC-MSCs (P15MSCs) and OE Beclin 1-P15 hUC-MSCs (OE Bec-P15MSCs) groups
(n=12). Overexpressing Beclin-1 significantly enhanced the proliferation and migration
of MSCs of different passages, and reduced G0/G1 arrest. The OE Bec-P15MSCs group
had the longest total distance, the shortest time in forced swimming test, and the largest
total food consumption in novelty suppressed feeding test. Transplanting hUC-MSCs
and overexpressing Beclin-1 significantly reduced Aβ deposition. The expressions
of P-tau (Ser396), P-tau (Ser231) and P-tau (Ser235) were significantly inhibited by
hUC-MSCs transplantation and Beclin-1 overexpression, especially in the OE Bec-
P15MSCs group. Double positive staining of EdU+/DCX+ cells, EdU+/NeuN+ cells and
EdU+/Nestin+ cells significantly increased in the OE Bec-P15MSCs group compared to
those in other groups (P<0.05). The activation degrees of astrocytes and microglia were
lowest and the superoxide dismutase activity was highest in the OE Bec-P15MSCs group
(P<0.05). The protein expression of nuclear factor E2-related factor 2 (Nrf2) in NAD(P)
H quinone oxidoreductase 1 and superoxide dismutase 1 in brain tissues significantly
rose, while that of Keap-1 was down-regulated in the OE Bec-P15MSCs group (P<0.05).
Beclin-1 can partly restore the viability of hUC-MSCs by activating the Nrf2 signaling
pathway, thereby enhancing the therapeutic effect of transplantation on AD mice. |
| first_indexed | 2024-03-08T14:23:43Z |
| format | Article |
| id | doaj.art-f126e7f268084becb45281d64364c250 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-08T14:23:43Z |
| publishDate | 2023-12-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-f126e7f268084becb45281d64364c2502024-01-13T17:59:33ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-12-0130151410.9775/kvfd.2023.300573061Beclin-1 improves the cognitive function of mice with alzheimer"s diseaseLi SHAO0Qing HE1Xin DU2Qing LI3Siyuan YANG4Chen DONG5Pengju MA6Department of Neurology, The Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou First People’s Hospital, Xuzhou 221116, Jiangsu Province, CHINADepartment of Neurology, The Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou First People’s Hospital, Xuzhou 221116, Jiangsu Province, CHINADepartment of Neurology, The Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou First People’s Hospital, Xuzhou 221116, Jiangsu Province, CHINADepartment of Neurology, The Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou First People’s Hospital, Xuzhou 221116, Jiangsu Province, CHINADepartment of Neurology, The Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou First People’s Hospital, Xuzhou 221116, Jiangsu Province, CHINADepartment of Neurology, The Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou First People’s Hospital, Xuzhou 221116, Jiangsu Province, CHINALinguistics and Applied Linguistics, School of Liberal Arts, Nanjing Normal University, Nanjing 210000, Jiangsu Province, CHINAWe aimed to investigate the improvement effect of Beclin-1 on the cognitive function of mice with Alzheimer"s disease (AD). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were isolated from neonatal umbilical cord tissues. The cells stably overexpressing Beclin-1 were constructed, and those of passages 5, 10, and 15 were used. Forty-eight AD mice were randomly divided into AD, P5 hUC-MSCs, P15 hUC-MSCs (P15MSCs) and OE Beclin 1-P15 hUC-MSCs (OE Bec-P15MSCs) groups (n=12). Overexpressing Beclin-1 significantly enhanced the proliferation and migration of MSCs of different passages, and reduced G0/G1 arrest. The OE Bec-P15MSCs group had the longest total distance, the shortest time in forced swimming test, and the largest total food consumption in novelty suppressed feeding test. Transplanting hUC-MSCs and overexpressing Beclin-1 significantly reduced Aβ deposition. The expressions of P-tau (Ser396), P-tau (Ser231) and P-tau (Ser235) were significantly inhibited by hUC-MSCs transplantation and Beclin-1 overexpression, especially in the OE Bec- P15MSCs group. Double positive staining of EdU+/DCX+ cells, EdU+/NeuN+ cells and EdU+/Nestin+ cells significantly increased in the OE Bec-P15MSCs group compared to those in other groups (P<0.05). The activation degrees of astrocytes and microglia were lowest and the superoxide dismutase activity was highest in the OE Bec-P15MSCs group (P<0.05). The protein expression of nuclear factor E2-related factor 2 (Nrf2) in NAD(P) H quinone oxidoreductase 1 and superoxide dismutase 1 in brain tissues significantly rose, while that of Keap-1 was down-regulated in the OE Bec-P15MSCs group (P<0.05). Beclin-1 can partly restore the viability of hUC-MSCs by activating the Nrf2 signaling pathway, thereby enhancing the therapeutic effect of transplantation on AD mice.https://vetdergikafkas.org/pdf.php?id=3061agingalzheimer"s diseasebeclin-1mesenchymal stem cell |
| spellingShingle | Li SHAO Qing HE Xin DU Qing LI Siyuan YANG Chen DONG Pengju MA Beclin-1 improves the cognitive function of mice with alzheimer"s disease Kafkas Universitesi Veteriner Fakültesi Dergisi aging alzheimer"s disease beclin-1 mesenchymal stem cell |
| title | Beclin-1 improves the cognitive function of mice with alzheimer"s disease |
| title_full | Beclin-1 improves the cognitive function of mice with alzheimer"s disease |
| title_fullStr | Beclin-1 improves the cognitive function of mice with alzheimer"s disease |
| title_full_unstemmed | Beclin-1 improves the cognitive function of mice with alzheimer"s disease |
| title_short | Beclin-1 improves the cognitive function of mice with alzheimer"s disease |
| title_sort | beclin 1 improves the cognitive function of mice with alzheimer s disease |
| topic | aging alzheimer"s disease beclin-1 mesenchymal stem cell |
| url | https://vetdergikafkas.org/pdf.php?id=3061 |
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