Structure of catalase determined by MicroED
MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of still diffraction patterns to determine the structure of lysozyme at 2.9 Å resolution with MicroED (Shi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2014-10-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/03600 |
| _version_ | 1811180508235694080 |
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| author | Brent L Nannenga Dan Shi Johan Hattne Francis E Reyes Tamir Gonen |
| author_facet | Brent L Nannenga Dan Shi Johan Hattne Francis E Reyes Tamir Gonen |
| author_sort | Brent L Nannenga |
| collection | DOAJ |
| description | MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of still diffraction patterns to determine the structure of lysozyme at 2.9 Å resolution with MicroED (Shi et al., 2013). Here we present the structure of bovine liver catalase determined from a single crystal at 3.2 Å resolution by MicroED. The data were collected by continuous rotation of the sample under constant exposure and were processed and refined using standard programs for X-ray crystallography. The ability of MicroED to determine the structure of bovine liver catalase, a protein that has long resisted atomic analysis by traditional electron crystallography, demonstrates the potential of this method for structure determination. |
| first_indexed | 2024-04-11T09:03:19Z |
| format | Article |
| id | doaj.art-f127022da7dc4abfa0e8386c3a47fb36 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:03:19Z |
| publishDate | 2014-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-f127022da7dc4abfa0e8386c3a47fb362022-12-22T04:32:42ZengeLife Sciences Publications LtdeLife2050-084X2014-10-01310.7554/eLife.03600Structure of catalase determined by MicroEDBrent L Nannenga0Dan Shi1Johan Hattne2https://orcid.org/0000-0002-8936-0912Francis E Reyes3Tamir Gonen4Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesMicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of still diffraction patterns to determine the structure of lysozyme at 2.9 Å resolution with MicroED (Shi et al., 2013). Here we present the structure of bovine liver catalase determined from a single crystal at 3.2 Å resolution by MicroED. The data were collected by continuous rotation of the sample under constant exposure and were processed and refined using standard programs for X-ray crystallography. The ability of MicroED to determine the structure of bovine liver catalase, a protein that has long resisted atomic analysis by traditional electron crystallography, demonstrates the potential of this method for structure determination.https://elifesciences.org/articles/03600MicroEDelectron diffractioncatalasemicro crystalelectron microscopycryo EM |
| spellingShingle | Brent L Nannenga Dan Shi Johan Hattne Francis E Reyes Tamir Gonen Structure of catalase determined by MicroED eLife MicroED electron diffraction catalase micro crystal electron microscopy cryo EM |
| title | Structure of catalase determined by MicroED |
| title_full | Structure of catalase determined by MicroED |
| title_fullStr | Structure of catalase determined by MicroED |
| title_full_unstemmed | Structure of catalase determined by MicroED |
| title_short | Structure of catalase determined by MicroED |
| title_sort | structure of catalase determined by microed |
| topic | MicroED electron diffraction catalase micro crystal electron microscopy cryo EM |
| url | https://elifesciences.org/articles/03600 |
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