Integrated transcriptome and metabolome analysis reveals that flavonoids function in wheat resistance to powdery mildew
Powdery mildew is a fungal disease devastating to wheat, causing significant quality and yield loss. Flavonoids are important secondary plant metabolites that confer resistance to biotic and abiotic stress. However, whether they play a role in powdery mildew resistance in wheat has yet to be explore...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1125194/full |
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| author | Wenjing Xu Wenjing Xu Wenjing Xu Wenjing Xu Xiaoyi Xu Ran Han Ran Han Ran Han Ran Han Xiaolu Wang Xiaolu Wang Xiaolu Wang Xiaolu Wang Kai Wang Kai Wang Kai Wang Kai Wang Guang Qi Guang Qi Guang Qi Guang Qi Pengtao Ma Takao Komatsuda Takao Komatsuda Takao Komatsuda Takao Komatsuda Cheng Liu Cheng Liu Cheng Liu Cheng Liu |
| author_facet | Wenjing Xu Wenjing Xu Wenjing Xu Wenjing Xu Xiaoyi Xu Ran Han Ran Han Ran Han Ran Han Xiaolu Wang Xiaolu Wang Xiaolu Wang Xiaolu Wang Kai Wang Kai Wang Kai Wang Kai Wang Guang Qi Guang Qi Guang Qi Guang Qi Pengtao Ma Takao Komatsuda Takao Komatsuda Takao Komatsuda Takao Komatsuda Cheng Liu Cheng Liu Cheng Liu Cheng Liu |
| author_sort | Wenjing Xu |
| collection | DOAJ |
| description | Powdery mildew is a fungal disease devastating to wheat, causing significant quality and yield loss. Flavonoids are important secondary plant metabolites that confer resistance to biotic and abiotic stress. However, whether they play a role in powdery mildew resistance in wheat has yet to be explored. In the present study, we combined transcriptome and metabolome analyses to compare differentially expressed genes (DEGs) and differentially accumulated flavonoids identified in plants with and without powdery mildew inoculation. Transcriptome analysis identified 4,329 DEGs in susceptible wheat cv. Jimai229, and 8,493 in resistant cv. HHG46. The DEGs were functionally enriched using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes, revealing the flavonoid synthesis pathway as the most significant in both cultivars. This was consistent with the upregulation of flavonoid synthesis pathway genes observed by quantitative PCR. Metabolome analysis indicated flavone and flavonol biosynthesis pathways as the most significantly enriched following powdery mildew inoculation. An accumulation of total flavonoids content was also found to be induced by powdery mildew infection. Exogenous flavonoids treatment of inoculated plants led to less severe infection, with fewer and smaller powdery mildew spots on the wheat leaves. This reduction is speculated to be regulated through malondialdehyde content and the activities of peroxidase and catalase. Our study provides a fundamental theory for further exploration of the potential of flavonoids as biological prevention and control agents against powdery mildew in wheat. |
| first_indexed | 2024-04-10T18:52:00Z |
| format | Article |
| id | doaj.art-f127729be11f47928452a61d56008187 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T18:52:00Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f127729be11f47928452a61d560081872023-02-01T07:03:42ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.11251941125194Integrated transcriptome and metabolome analysis reveals that flavonoids function in wheat resistance to powdery mildewWenjing Xu0Wenjing Xu1Wenjing Xu2Wenjing Xu3Xiaoyi Xu4Ran Han5Ran Han6Ran Han7Ran Han8Xiaolu Wang9Xiaolu Wang10Xiaolu Wang11Xiaolu Wang12Kai Wang13Kai Wang14Kai Wang15Kai Wang16Guang Qi17Guang Qi18Guang Qi19Guang Qi20Pengtao Ma21Takao Komatsuda22Takao Komatsuda23Takao Komatsuda24Takao Komatsuda25Cheng Liu26Cheng Liu27Cheng Liu28Cheng Liu29Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaNational Engineering Laboratory of Wheat and Maize, Jinan, Shandong, ChinaKey Laboratory of Wheat Biology and Genetic Improvement in the North Huang and Huai River Valley of Ministry of Agriculture, Jinan, Shandong, ChinaShandong Wheat Technology Innovation Center, Jinan, Shandong, ChinaSchool of Life Sciences, Yantai University, Yantai, Shandong, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaNational Engineering Laboratory of Wheat and Maize, Jinan, Shandong, ChinaKey Laboratory of Wheat Biology and Genetic Improvement in the North Huang and Huai River Valley of Ministry of Agriculture, Jinan, Shandong, ChinaShandong Wheat Technology Innovation Center, Jinan, Shandong, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaNational Engineering Laboratory of Wheat and Maize, Jinan, Shandong, ChinaKey Laboratory of Wheat Biology and Genetic Improvement in the North Huang and Huai River Valley of Ministry of Agriculture, Jinan, Shandong, ChinaShandong Wheat Technology Innovation Center, Jinan, Shandong, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaNational Engineering Laboratory of Wheat and Maize, Jinan, Shandong, ChinaKey Laboratory of Wheat Biology and Genetic Improvement in the North Huang and Huai River Valley of Ministry of Agriculture, Jinan, Shandong, ChinaShandong Wheat Technology Innovation Center, Jinan, Shandong, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaNational Engineering Laboratory of Wheat and Maize, Jinan, Shandong, ChinaKey Laboratory of Wheat Biology and Genetic Improvement in the North Huang and Huai River Valley of Ministry of Agriculture, Jinan, Shandong, ChinaShandong Wheat Technology Innovation Center, Jinan, Shandong, ChinaSchool of Life Sciences, Yantai University, Yantai, Shandong, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaNational Engineering Laboratory of Wheat and Maize, Jinan, Shandong, ChinaKey Laboratory of Wheat Biology and Genetic Improvement in the North Huang and Huai River Valley of Ministry of Agriculture, Jinan, Shandong, ChinaShandong Wheat Technology Innovation Center, Jinan, Shandong, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaNational Engineering Laboratory of Wheat and Maize, Jinan, Shandong, ChinaKey Laboratory of Wheat Biology and Genetic Improvement in the North Huang and Huai River Valley of Ministry of Agriculture, Jinan, Shandong, ChinaShandong Wheat Technology Innovation Center, Jinan, Shandong, ChinaPowdery mildew is a fungal disease devastating to wheat, causing significant quality and yield loss. Flavonoids are important secondary plant metabolites that confer resistance to biotic and abiotic stress. However, whether they play a role in powdery mildew resistance in wheat has yet to be explored. In the present study, we combined transcriptome and metabolome analyses to compare differentially expressed genes (DEGs) and differentially accumulated flavonoids identified in plants with and without powdery mildew inoculation. Transcriptome analysis identified 4,329 DEGs in susceptible wheat cv. Jimai229, and 8,493 in resistant cv. HHG46. The DEGs were functionally enriched using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes, revealing the flavonoid synthesis pathway as the most significant in both cultivars. This was consistent with the upregulation of flavonoid synthesis pathway genes observed by quantitative PCR. Metabolome analysis indicated flavone and flavonol biosynthesis pathways as the most significantly enriched following powdery mildew inoculation. An accumulation of total flavonoids content was also found to be induced by powdery mildew infection. Exogenous flavonoids treatment of inoculated plants led to less severe infection, with fewer and smaller powdery mildew spots on the wheat leaves. This reduction is speculated to be regulated through malondialdehyde content and the activities of peroxidase and catalase. Our study provides a fundamental theory for further exploration of the potential of flavonoids as biological prevention and control agents against powdery mildew in wheat.https://www.frontiersin.org/articles/10.3389/fpls.2023.1125194/fullwheatpowdery mildewtranscriptomemetabolomeflavonoids |
| spellingShingle | Wenjing Xu Wenjing Xu Wenjing Xu Wenjing Xu Xiaoyi Xu Ran Han Ran Han Ran Han Ran Han Xiaolu Wang Xiaolu Wang Xiaolu Wang Xiaolu Wang Kai Wang Kai Wang Kai Wang Kai Wang Guang Qi Guang Qi Guang Qi Guang Qi Pengtao Ma Takao Komatsuda Takao Komatsuda Takao Komatsuda Takao Komatsuda Cheng Liu Cheng Liu Cheng Liu Cheng Liu Integrated transcriptome and metabolome analysis reveals that flavonoids function in wheat resistance to powdery mildew Frontiers in Plant Science wheat powdery mildew transcriptome metabolome flavonoids |
| title | Integrated transcriptome and metabolome analysis reveals that flavonoids function in wheat resistance to powdery mildew |
| title_full | Integrated transcriptome and metabolome analysis reveals that flavonoids function in wheat resistance to powdery mildew |
| title_fullStr | Integrated transcriptome and metabolome analysis reveals that flavonoids function in wheat resistance to powdery mildew |
| title_full_unstemmed | Integrated transcriptome and metabolome analysis reveals that flavonoids function in wheat resistance to powdery mildew |
| title_short | Integrated transcriptome and metabolome analysis reveals that flavonoids function in wheat resistance to powdery mildew |
| title_sort | integrated transcriptome and metabolome analysis reveals that flavonoids function in wheat resistance to powdery mildew |
| topic | wheat powdery mildew transcriptome metabolome flavonoids |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1125194/full |
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