Microbial Community Composition Analysis in Spring Aerosols at Urban and Remote Sites over the Tibetan Plateau
This study presents features of airborne culturable bacteria and fungi from three different sites (Lanzhou; LZ; 1520 m ASL, Lhasa; LS; 3640 m ASL and Qomolangma; ZF; 4276 m ASL) representing urban (LZ and LS) and remote sites (ZF) over the Tibetan Plateau (TP). Total suspended particle (TSP) samples...
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MDPI AG
2020-05-01
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| Series: | Atmosphere |
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| Online Access: | https://www.mdpi.com/2073-4433/11/5/527 |
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| author | Prakriti Sharma Ghimire Shichang Kang Wasim Sajjad Barkat Ali Lekhendra Tripathee Pengfei Chen |
| author_facet | Prakriti Sharma Ghimire Shichang Kang Wasim Sajjad Barkat Ali Lekhendra Tripathee Pengfei Chen |
| author_sort | Prakriti Sharma Ghimire |
| collection | DOAJ |
| description | This study presents features of airborne culturable bacteria and fungi from three different sites (Lanzhou; LZ; 1520 m ASL, Lhasa; LS; 3640 m ASL and Qomolangma; ZF; 4276 m ASL) representing urban (LZ and LS) and remote sites (ZF) over the Tibetan Plateau (TP). Total suspended particle (TSP) samples were collected with an air sampler (Laoying 2030, China) on a quartz filter. Community structures of bacteria and fungi were studied and compared among three different locations. The average levels of bacterial load in the outdoor air ranged from approximately 8.03 × 10<sup>1</sup> to 3.25 × 10<sup>2</sup> CFU m<sup>–3</sup> (Colony forming unit per m<sup>3</sup>). However, the average levels of fungal loads ranged from approximately 3.88 × 10<sup>0</sup> to 1.55 × 10<sup>1</sup> CFU m<sup>−3</sup>. Bacterial load was one magnitude higher at urban sites LZ (2.06 × 10<sup>2</sup>–3.25 × 10<sup>2</sup> CFU m<sup>−3</sup>) and LS (1.96 × 10<sup>2</sup>–3.23 × 10<sup>2</sup> CFU m<sup>−3</sup>) compared to remote sites ZF (8.03 × 10<sup>1</sup>–9.54 × 10<sup>1</sup> CFU m<sup>−3</sup>). Similarly, the maximum fungal load was observed in LZ (1.02 × 10<sup>1</sup>–1.55 × 10<sup>1</sup> CFU m<sup>−3</sup>) followed by LS (1.03 × 10<sup>1</sup>–1.49 × 10<sup>1</sup> CFU m<sup>−3</sup>) and ZF (3.88 × 10<sup>0</sup>–6.26 × 10<sup>0</sup> CFU m<sup>−3</sup>). However, the maximum microbial concentration was observed on the same day of the month, corresponding to a high dust storm in Lanzhou during the sampling period. The reported isolates were identified by phylogenetic analysis of 16S rRNA genes for bacteria and ITS sequences for fungi amplified from directly extracted DNA. Bacterial isolates were mostly associated with <i>Proteobacteria,</i> <i>Eurotiomycetes and Bacillus</i>, whereas fungal isolates were mostly <i>Aspergillus</i> and <i>Alternaria</i>. Overall, this is a pioneer study that provides information about the airborne microbial concentration and composition of three sites over the TP region depending on environmental parameters. This study provided preliminary insight to carry out more advanced and targeted analyses of bioaerosol in the sites presented in the study. |
| first_indexed | 2024-03-10T19:43:20Z |
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| issn | 2073-4433 |
| language | English |
| last_indexed | 2024-03-10T19:43:20Z |
| publishDate | 2020-05-01 |
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| series | Atmosphere |
| spelling | doaj.art-f127bd1c5cf144eab45917ad0a7cae9f2023-11-20T01:05:20ZengMDPI AGAtmosphere2073-44332020-05-0111552710.3390/atmos11050527Microbial Community Composition Analysis in Spring Aerosols at Urban and Remote Sites over the Tibetan PlateauPrakriti Sharma Ghimire0Shichang Kang1Wasim Sajjad2Barkat Ali3Lekhendra Tripathee4Pengfei Chen5State Key Laboratory of Cryospheric Science, Northwest Institute of Eco-environment and Resources, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaState Key Laboratory of Cryospheric Science, Northwest Institute of Eco-environment and Resources, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaState Key Laboratory of Cryospheric Science, Northwest Institute of Eco-environment and Resources, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaState Key Laboratory of Cryospheric Science, Northwest Institute of Eco-environment and Resources, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaState Key Laboratory of Cryospheric Science, Northwest Institute of Eco-environment and Resources, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaState Key Laboratory of Cryospheric Science, Northwest Institute of Eco-environment and Resources, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaThis study presents features of airborne culturable bacteria and fungi from three different sites (Lanzhou; LZ; 1520 m ASL, Lhasa; LS; 3640 m ASL and Qomolangma; ZF; 4276 m ASL) representing urban (LZ and LS) and remote sites (ZF) over the Tibetan Plateau (TP). Total suspended particle (TSP) samples were collected with an air sampler (Laoying 2030, China) on a quartz filter. Community structures of bacteria and fungi were studied and compared among three different locations. The average levels of bacterial load in the outdoor air ranged from approximately 8.03 × 10<sup>1</sup> to 3.25 × 10<sup>2</sup> CFU m<sup>–3</sup> (Colony forming unit per m<sup>3</sup>). However, the average levels of fungal loads ranged from approximately 3.88 × 10<sup>0</sup> to 1.55 × 10<sup>1</sup> CFU m<sup>−3</sup>. Bacterial load was one magnitude higher at urban sites LZ (2.06 × 10<sup>2</sup>–3.25 × 10<sup>2</sup> CFU m<sup>−3</sup>) and LS (1.96 × 10<sup>2</sup>–3.23 × 10<sup>2</sup> CFU m<sup>−3</sup>) compared to remote sites ZF (8.03 × 10<sup>1</sup>–9.54 × 10<sup>1</sup> CFU m<sup>−3</sup>). Similarly, the maximum fungal load was observed in LZ (1.02 × 10<sup>1</sup>–1.55 × 10<sup>1</sup> CFU m<sup>−3</sup>) followed by LS (1.03 × 10<sup>1</sup>–1.49 × 10<sup>1</sup> CFU m<sup>−3</sup>) and ZF (3.88 × 10<sup>0</sup>–6.26 × 10<sup>0</sup> CFU m<sup>−3</sup>). However, the maximum microbial concentration was observed on the same day of the month, corresponding to a high dust storm in Lanzhou during the sampling period. The reported isolates were identified by phylogenetic analysis of 16S rRNA genes for bacteria and ITS sequences for fungi amplified from directly extracted DNA. Bacterial isolates were mostly associated with <i>Proteobacteria,</i> <i>Eurotiomycetes and Bacillus</i>, whereas fungal isolates were mostly <i>Aspergillus</i> and <i>Alternaria</i>. Overall, this is a pioneer study that provides information about the airborne microbial concentration and composition of three sites over the TP region depending on environmental parameters. This study provided preliminary insight to carry out more advanced and targeted analyses of bioaerosol in the sites presented in the study.https://www.mdpi.com/2073-4433/11/5/527bioaerosoldiversityTibetan Plateaumicrobial communityculturable microorganisms |
| spellingShingle | Prakriti Sharma Ghimire Shichang Kang Wasim Sajjad Barkat Ali Lekhendra Tripathee Pengfei Chen Microbial Community Composition Analysis in Spring Aerosols at Urban and Remote Sites over the Tibetan Plateau Atmosphere bioaerosol diversity Tibetan Plateau microbial community culturable microorganisms |
| title | Microbial Community Composition Analysis in Spring Aerosols at Urban and Remote Sites over the Tibetan Plateau |
| title_full | Microbial Community Composition Analysis in Spring Aerosols at Urban and Remote Sites over the Tibetan Plateau |
| title_fullStr | Microbial Community Composition Analysis in Spring Aerosols at Urban and Remote Sites over the Tibetan Plateau |
| title_full_unstemmed | Microbial Community Composition Analysis in Spring Aerosols at Urban and Remote Sites over the Tibetan Plateau |
| title_short | Microbial Community Composition Analysis in Spring Aerosols at Urban and Remote Sites over the Tibetan Plateau |
| title_sort | microbial community composition analysis in spring aerosols at urban and remote sites over the tibetan plateau |
| topic | bioaerosol diversity Tibetan Plateau microbial community culturable microorganisms |
| url | https://www.mdpi.com/2073-4433/11/5/527 |
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