Deep Sequencing of Small RNAs in the Whitefly <i>Bemisia tabaci</i> Reveals Novel MicroRNAs Potentially Associated with Begomovirus Acquisition and Transmission
The whitefly <i>Bemisia tabaci</i> (Gennadius) is a notorious insect vector that transmits hundreds of plant viruses, affecting food and fiber crops worldwide, and results in the equivalent of billions of U.S. dollars in crop loss annually. To gain a better understanding of the mechanism...
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MDPI AG
2020-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/9/562 |
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| author | Daniel K. Hasegawa Md Shamimuzzaman Wenbo Chen Alvin M. Simmons Zhangjun Fei Kai-Shu Ling |
| author_facet | Daniel K. Hasegawa Md Shamimuzzaman Wenbo Chen Alvin M. Simmons Zhangjun Fei Kai-Shu Ling |
| author_sort | Daniel K. Hasegawa |
| collection | DOAJ |
| description | The whitefly <i>Bemisia tabaci</i> (Gennadius) is a notorious insect vector that transmits hundreds of plant viruses, affecting food and fiber crops worldwide, and results in the equivalent of billions of U.S. dollars in crop loss annually. To gain a better understanding of the mechanism in virus transmission, we conducted deep sequencing of small RNAs on the whitefly <i>B. tabaci</i> MEAM1 (Middle East-Asia Minor 1) that fed on tomato plants infected with tomato yellow leaf curl virus (TYLCV). Overall, 160 miRNAs were identified, 66 of which were conserved and 94 were <i>B. tabaci</i>-specific. Among the <i>B. tabaci</i>-specific miRNAs, 67 were newly described in the present study. Two miRNAs, with predicted targets encoding a nuclear receptor (<i>Bta05482</i>) and a very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 (<i>Bta10702</i>), respectively, were differentially expressed in whiteflies that fed on TYLCV-infected versus uninfected plants. To better understand the regulatory effects of identified miRNAs and their target genes, we correlated expression profiles of miRNAs and their target transcripts and found that, interestingly, miRNA expression was inversely correlated with the expression of ~50% of the predicted target genes. These analyses could serve as a model to study gene regulation in other systems involving arthropod transmission of viruses to plants and animals. |
| first_indexed | 2024-03-10T16:57:24Z |
| format | Article |
| id | doaj.art-f127d4ca2b30486798104d33e9775703 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T16:57:24Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-f127d4ca2b30486798104d33e97757032023-11-20T11:05:31ZengMDPI AGInsects2075-44502020-08-0111956210.3390/insects11090562Deep Sequencing of Small RNAs in the Whitefly <i>Bemisia tabaci</i> Reveals Novel MicroRNAs Potentially Associated with Begomovirus Acquisition and TransmissionDaniel K. Hasegawa0Md Shamimuzzaman1Wenbo Chen2Alvin M. Simmons3Zhangjun Fei4Kai-Shu Ling5USDA, Agricultural Research Service, U.S. Vegetable Laboratory, 2700 Savannah Hwy, Charleston, SC 29414, USAUSDA, Agricultural Research Service, U.S. Vegetable Laboratory, 2700 Savannah Hwy, Charleston, SC 29414, USABoyce Thompson Institute, 533 Tower Road, Ithaca, NY 14853, USAUSDA, Agricultural Research Service, U.S. Vegetable Laboratory, 2700 Savannah Hwy, Charleston, SC 29414, USABoyce Thompson Institute, 533 Tower Road, Ithaca, NY 14853, USAUSDA, Agricultural Research Service, U.S. Vegetable Laboratory, 2700 Savannah Hwy, Charleston, SC 29414, USAThe whitefly <i>Bemisia tabaci</i> (Gennadius) is a notorious insect vector that transmits hundreds of plant viruses, affecting food and fiber crops worldwide, and results in the equivalent of billions of U.S. dollars in crop loss annually. To gain a better understanding of the mechanism in virus transmission, we conducted deep sequencing of small RNAs on the whitefly <i>B. tabaci</i> MEAM1 (Middle East-Asia Minor 1) that fed on tomato plants infected with tomato yellow leaf curl virus (TYLCV). Overall, 160 miRNAs were identified, 66 of which were conserved and 94 were <i>B. tabaci</i>-specific. Among the <i>B. tabaci</i>-specific miRNAs, 67 were newly described in the present study. Two miRNAs, with predicted targets encoding a nuclear receptor (<i>Bta05482</i>) and a very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 (<i>Bta10702</i>), respectively, were differentially expressed in whiteflies that fed on TYLCV-infected versus uninfected plants. To better understand the regulatory effects of identified miRNAs and their target genes, we correlated expression profiles of miRNAs and their target transcripts and found that, interestingly, miRNA expression was inversely correlated with the expression of ~50% of the predicted target genes. These analyses could serve as a model to study gene regulation in other systems involving arthropod transmission of viruses to plants and animals.https://www.mdpi.com/2075-4450/11/9/562whitefly<i>Bemisia tabaci</i>tomato yellow leaf curl virusTYLCVmicroRNA (miRNA) |
| spellingShingle | Daniel K. Hasegawa Md Shamimuzzaman Wenbo Chen Alvin M. Simmons Zhangjun Fei Kai-Shu Ling Deep Sequencing of Small RNAs in the Whitefly <i>Bemisia tabaci</i> Reveals Novel MicroRNAs Potentially Associated with Begomovirus Acquisition and Transmission Insects whitefly <i>Bemisia tabaci</i> tomato yellow leaf curl virus TYLCV microRNA (miRNA) |
| title | Deep Sequencing of Small RNAs in the Whitefly <i>Bemisia tabaci</i> Reveals Novel MicroRNAs Potentially Associated with Begomovirus Acquisition and Transmission |
| title_full | Deep Sequencing of Small RNAs in the Whitefly <i>Bemisia tabaci</i> Reveals Novel MicroRNAs Potentially Associated with Begomovirus Acquisition and Transmission |
| title_fullStr | Deep Sequencing of Small RNAs in the Whitefly <i>Bemisia tabaci</i> Reveals Novel MicroRNAs Potentially Associated with Begomovirus Acquisition and Transmission |
| title_full_unstemmed | Deep Sequencing of Small RNAs in the Whitefly <i>Bemisia tabaci</i> Reveals Novel MicroRNAs Potentially Associated with Begomovirus Acquisition and Transmission |
| title_short | Deep Sequencing of Small RNAs in the Whitefly <i>Bemisia tabaci</i> Reveals Novel MicroRNAs Potentially Associated with Begomovirus Acquisition and Transmission |
| title_sort | deep sequencing of small rnas in the whitefly i bemisia tabaci i reveals novel micrornas potentially associated with begomovirus acquisition and transmission |
| topic | whitefly <i>Bemisia tabaci</i> tomato yellow leaf curl virus TYLCV microRNA (miRNA) |
| url | https://www.mdpi.com/2075-4450/11/9/562 |
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