Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors
We describe the generation and characterisation of five human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123000958 |
| _version_ | 1797815951676145664 |
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| author | Katerina Vlahos Koula Sourris Jia Yi Kuah Alison Graham Annabelle Suter Sara E. Howden Edouard G. Stanley Andrew G. Elefanty |
| author_facet | Katerina Vlahos Koula Sourris Jia Yi Kuah Alison Graham Annabelle Suter Sara E. Howden Edouard G. Stanley Andrew G. Elefanty |
| author_sort | Katerina Vlahos |
| collection | DOAJ |
| description | We describe the generation and characterisation of five human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, and pluripotency was validated by flow cytometry and immunofluorescence of pluripotency markers, and their differentiation into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms. |
| first_indexed | 2024-03-13T08:30:23Z |
| format | Article |
| id | doaj.art-f127ec15d76c4fbab92c69a6aa7bc4ae |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-13T08:30:23Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-f127ec15d76c4fbab92c69a6aa7bc4ae2023-05-31T04:44:14ZengElsevierStem Cell Research1873-50612023-06-0169103109Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donorsKaterina Vlahos0Koula Sourris1Jia Yi Kuah2Alison Graham3Annabelle Suter4Sara E. Howden5Edouard G. Stanley6Andrew G. Elefanty7Murdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia; Corresponding author.Murdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, AustraliaMurdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, AustraliaMurdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, AustraliaMurdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, AustraliaMurdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children’s Research Institute, Parkville, VIC, AustraliaMurdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children’s Research Institute, Parkville, VIC, AustraliaMurdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children’s Research Institute, Parkville, VIC, AustraliaWe describe the generation and characterisation of five human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, and pluripotency was validated by flow cytometry and immunofluorescence of pluripotency markers, and their differentiation into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.http://www.sciencedirect.com/science/article/pii/S1873506123000958 |
| spellingShingle | Katerina Vlahos Koula Sourris Jia Yi Kuah Alison Graham Annabelle Suter Sara E. Howden Edouard G. Stanley Andrew G. Elefanty Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors Stem Cell Research |
| title | Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors |
| title_full | Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors |
| title_fullStr | Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors |
| title_full_unstemmed | Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors |
| title_short | Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors |
| title_sort | generation of ipsc lines from peripheral blood mononuclear cells from five healthy donors |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123000958 |
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