Characteristics of biofilms associated with enhanced survival of Campylobacter jejuni
The biofilm microflora isolated from a chicken house (Y1 and W1) and a meat plant (Pseudomonas sp.) enhanced survival of C. jejuni in a low nutrient environment. We employed direct microscopic observation techniques to accomplish this objective. Staining with cyanoditolyltetrazolium chloride CTC) in...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2005-11-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/27-6-pdf/13-Campylobacter-jejuni.pdf |
| _version_ | 1811319253712764928 |
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| author | Nathanon Trachoo Joseph F. Frank |
| author_facet | Nathanon Trachoo Joseph F. Frank |
| author_sort | Nathanon Trachoo |
| collection | DOAJ |
| description | The biofilm microflora isolated from a chicken house (Y1 and W1) and a meat plant (Pseudomonas sp.) enhanced survival of C. jejuni in a low nutrient environment. We employed direct microscopic observation techniques to accomplish this objective. Staining with cyanoditolyltetrazolium chloride CTC) indicated reduced oxygen tension in the biofilm environment, which could enhance the survival of C. jejuni. W1 biofilms with more CTC staining showed greater enhancement of C. jejuni survival than did Pseudomonas sp biofilm with more area coverage. This may indicate that reduced oxygen in biofilm of W1 plays important role in enhanced survival of C. jejuni. The Pseudomonas sp. biofilm exhibited the most extensive surface coverage and was thinner (approximately 1 μm) than Y1 and W1 biofilms. Reconstructed three-dimensional photomicrographs showed only one layer of cells in Pseudomonas sp. biofilm, while biofilms of W1 and Y1 were more complex. They consisted of different sizes of microcolonies with different thickness and void spaces in between. These morphological and physiological properties of the biofilms may be associated with increased survival of C. jejuni by providing improved nutrient entrapment and environmental stress protection, but no characteristic by itself could explain enhanced survival, which appears to be a complex phenomenon. |
| first_indexed | 2024-04-13T12:39:32Z |
| format | Article |
| id | doaj.art-f128632b70aa43c0827cda1195e8ba68 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-13T12:39:32Z |
| publishDate | 2005-11-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-f128632b70aa43c0827cda1195e8ba682022-12-22T02:46:33ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952005-11-0127612431252Characteristics of biofilms associated with enhanced survival of Campylobacter jejuniNathanon TrachooJoseph F. FrankThe biofilm microflora isolated from a chicken house (Y1 and W1) and a meat plant (Pseudomonas sp.) enhanced survival of C. jejuni in a low nutrient environment. We employed direct microscopic observation techniques to accomplish this objective. Staining with cyanoditolyltetrazolium chloride CTC) indicated reduced oxygen tension in the biofilm environment, which could enhance the survival of C. jejuni. W1 biofilms with more CTC staining showed greater enhancement of C. jejuni survival than did Pseudomonas sp biofilm with more area coverage. This may indicate that reduced oxygen in biofilm of W1 plays important role in enhanced survival of C. jejuni. The Pseudomonas sp. biofilm exhibited the most extensive surface coverage and was thinner (approximately 1 μm) than Y1 and W1 biofilms. Reconstructed three-dimensional photomicrographs showed only one layer of cells in Pseudomonas sp. biofilm, while biofilms of W1 and Y1 were more complex. They consisted of different sizes of microcolonies with different thickness and void spaces in between. These morphological and physiological properties of the biofilms may be associated with increased survival of C. jejuni by providing improved nutrient entrapment and environmental stress protection, but no characteristic by itself could explain enhanced survival, which appears to be a complex phenomenon.http://www.sjst.psu.ac.th/journal/27-6-pdf/13-Campylobacter-jejuni.pdfCampylobacter jejuniCTCbiofilm |
| spellingShingle | Nathanon Trachoo Joseph F. Frank Characteristics of biofilms associated with enhanced survival of Campylobacter jejuni Songklanakarin Journal of Science and Technology (SJST) Campylobacter jejuni CTC biofilm |
| title | Characteristics of biofilms associated with enhanced survival of Campylobacter jejuni |
| title_full | Characteristics of biofilms associated with enhanced survival of Campylobacter jejuni |
| title_fullStr | Characteristics of biofilms associated with enhanced survival of Campylobacter jejuni |
| title_full_unstemmed | Characteristics of biofilms associated with enhanced survival of Campylobacter jejuni |
| title_short | Characteristics of biofilms associated with enhanced survival of Campylobacter jejuni |
| title_sort | characteristics of biofilms associated with enhanced survival of campylobacter jejuni |
| topic | Campylobacter jejuni CTC biofilm |
| url | http://www.sjst.psu.ac.th/journal/27-6-pdf/13-Campylobacter-jejuni.pdf |
| work_keys_str_mv | AT nathanontrachoo characteristicsofbiofilmsassociatedwithenhancedsurvivalofcampylobacterjejuni AT josephffrank characteristicsofbiofilmsassociatedwithenhancedsurvivalofcampylobacterjejuni |