Antibiotic-Resistant Escherichia coli Isolated from Duck Cloacal and Tap Water Samples at Live Bird Markets in Bangladesh
Antibiotic resistance is a growing concern all over the world. The current study sought to identify antimicrobial resistance (AMR) patterns and antibiotic-resistant genes in Escherichia coli (E. coli) isolated from seemingly healthy ducks and neighboring tap water sources at three separate live bird...
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Gorgan University of Agricultural Sciences and Natural Resources
2023-01-01
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| Series: | Poultry Science Journal |
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| Online Access: | https://psj.gau.ac.ir/article_6254_2693df8ea95d65dba4d4f58ee7b7493b.pdf |
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| author | Md Samun Sarker Md Ehsanul Kabir Fatema Akter Mahua Ayesha Ahmed Rezaul Karim ipon Vanessa Silva Patricia Poeta Abdul Ahad |
| author_facet | Md Samun Sarker Md Ehsanul Kabir Fatema Akter Mahua Ayesha Ahmed Rezaul Karim ipon Vanessa Silva Patricia Poeta Abdul Ahad |
| author_sort | Md Samun Sarker |
| collection | DOAJ |
| description | Antibiotic resistance is a growing concern all over the world. The current study sought to identify antimicrobial resistance (AMR) patterns and antibiotic-resistant genes in Escherichia coli (E. coli) isolated from seemingly healthy ducks and neighboring tap water sources at three separate live bird markets (LBMs) in Chattogram, Bangladesh. A total of ninety cloacal swab samples of Khaki Campbell ducks and fifteen water samples from nearby tap water sources were collected from three LBMs. Several cultural and molecular tests were conducted to determine E. coli contamination. The disk diffusion technique was used to evaluate the antibiotic sensitivity of E. coli isolates to 12 different antibiotics. For each isolate, a Multiple Antibiotic Resistance (MAR) index was calculated. The resistance genes were detected using a polymerase chain reaction (PCR) assay. The overall prevalence of E. coli in feces and tap water samples was 64.4% (58/90, 95% CI 54.1-73.6) and 100% (15/15, 95% CI 76.1-100), respectively. Both fecal and water isolates showed 100% resistance to ampicillin, tetracycline, and nalidixic acid. Resistance to other antibiotics was also found to be high. Multidrug- resistance (MDR) was unveiled in all fecal (58/58) and water (15/15) isolates. MAR index ranged from 0.33 to 0.67 in all recovered isolates. Both fecal and water E. coli isolates harbored blaTEM, tetA, sul1, and sul2 genes. The resistance genes in MDR E. coli in live bird markets might transmit from ducks to humans and they, therefore local authorities should consider this issue a major public health risk. |
| first_indexed | 2024-04-10T20:56:11Z |
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| id | doaj.art-f1292a4e105d42c7a0ee490f205eff2b |
| institution | Directory Open Access Journal |
| issn | 2345-6604 2345-6566 |
| language | English |
| last_indexed | 2024-04-10T20:56:11Z |
| publishDate | 2023-01-01 |
| publisher | Gorgan University of Agricultural Sciences and Natural Resources |
| record_format | Article |
| series | Poultry Science Journal |
| spelling | doaj.art-f1292a4e105d42c7a0ee490f205eff2b2023-01-23T06:30:55ZengGorgan University of Agricultural Sciences and Natural ResourcesPoultry Science Journal2345-66042345-65662023-01-01111192710.22069/psj.2022.20028.17896254Antibiotic-Resistant Escherichia coli Isolated from Duck Cloacal and Tap Water Samples at Live Bird Markets in BangladeshMd Samun Sarker0Md Ehsanul Kabir1Fatema Akter Mahua2Ayesha Ahmed3Rezaul Karim ipon4Vanessa Silva5Patricia Poeta6Abdul Ahad7Antimicrobial Resistance Action Center (ARAC), Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka-1341, BangladeshAntimicrobial Resistance Action Center (ARAC), Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka-1341, BangladeshAntimicrobial Resistance Action Center (ARAC), Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka-1341, BangladeshAntimicrobial Resistance Action Center (ARAC), Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka-1341, BangladeshDepartment of Public Health and Informatics, Jahangirnagar University, Savar, Dhaka-1342, BangladeshMicrobiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalMicrobiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalDepartment of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram-4225, BangladeshAntibiotic resistance is a growing concern all over the world. The current study sought to identify antimicrobial resistance (AMR) patterns and antibiotic-resistant genes in Escherichia coli (E. coli) isolated from seemingly healthy ducks and neighboring tap water sources at three separate live bird markets (LBMs) in Chattogram, Bangladesh. A total of ninety cloacal swab samples of Khaki Campbell ducks and fifteen water samples from nearby tap water sources were collected from three LBMs. Several cultural and molecular tests were conducted to determine E. coli contamination. The disk diffusion technique was used to evaluate the antibiotic sensitivity of E. coli isolates to 12 different antibiotics. For each isolate, a Multiple Antibiotic Resistance (MAR) index was calculated. The resistance genes were detected using a polymerase chain reaction (PCR) assay. The overall prevalence of E. coli in feces and tap water samples was 64.4% (58/90, 95% CI 54.1-73.6) and 100% (15/15, 95% CI 76.1-100), respectively. Both fecal and water isolates showed 100% resistance to ampicillin, tetracycline, and nalidixic acid. Resistance to other antibiotics was also found to be high. Multidrug- resistance (MDR) was unveiled in all fecal (58/58) and water (15/15) isolates. MAR index ranged from 0.33 to 0.67 in all recovered isolates. Both fecal and water E. coli isolates harbored blaTEM, tetA, sul1, and sul2 genes. The resistance genes in MDR E. coli in live bird markets might transmit from ducks to humans and they, therefore local authorities should consider this issue a major public health risk.https://psj.gau.ac.ir/article_6254_2693df8ea95d65dba4d4f58ee7b7493b.pdfducke. colipublic healthlive bird marketantibiotic resistance |
| spellingShingle | Md Samun Sarker Md Ehsanul Kabir Fatema Akter Mahua Ayesha Ahmed Rezaul Karim ipon Vanessa Silva Patricia Poeta Abdul Ahad Antibiotic-Resistant Escherichia coli Isolated from Duck Cloacal and Tap Water Samples at Live Bird Markets in Bangladesh Poultry Science Journal duck e. coli public health live bird market antibiotic resistance |
| title | Antibiotic-Resistant Escherichia coli Isolated from Duck Cloacal and Tap Water Samples at Live Bird Markets in Bangladesh |
| title_full | Antibiotic-Resistant Escherichia coli Isolated from Duck Cloacal and Tap Water Samples at Live Bird Markets in Bangladesh |
| title_fullStr | Antibiotic-Resistant Escherichia coli Isolated from Duck Cloacal and Tap Water Samples at Live Bird Markets in Bangladesh |
| title_full_unstemmed | Antibiotic-Resistant Escherichia coli Isolated from Duck Cloacal and Tap Water Samples at Live Bird Markets in Bangladesh |
| title_short | Antibiotic-Resistant Escherichia coli Isolated from Duck Cloacal and Tap Water Samples at Live Bird Markets in Bangladesh |
| title_sort | antibiotic resistant escherichia coli isolated from duck cloacal and tap water samples at live bird markets in bangladesh |
| topic | duck e. coli public health live bird market antibiotic resistance |
| url | https://psj.gau.ac.ir/article_6254_2693df8ea95d65dba4d4f58ee7b7493b.pdf |
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