Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua
Summary: Various human diseases and pregnancy-related disorders reflect endometrial dysfunction. However, rodent models do not share fundamental biological processes with the human endometrium, such as spontaneous decidualization, and no existing human cell cultures recapitulate the cyclic interacti...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-05-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124721004770 |
| _version_ | 1818455358072422400 |
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| author | Virginia Chu Cheung Chian-Yu Peng Mirna Marinić Noboru J. Sakabe Ivy Aneas Vincent J. Lynch Carole Ober Marcelo A. Nobrega John A. Kessler |
| author_facet | Virginia Chu Cheung Chian-Yu Peng Mirna Marinić Noboru J. Sakabe Ivy Aneas Vincent J. Lynch Carole Ober Marcelo A. Nobrega John A. Kessler |
| author_sort | Virginia Chu Cheung |
| collection | DOAJ |
| description | Summary: Various human diseases and pregnancy-related disorders reflect endometrial dysfunction. However, rodent models do not share fundamental biological processes with the human endometrium, such as spontaneous decidualization, and no existing human cell cultures recapitulate the cyclic interactions between endometrial stromal and epithelial compartments necessary for decidualization and implantation. Here we report a protocol differentiating human pluripotent stem cells into endometrial stromal fibroblasts (PSC-ESFs) that are highly pure and able to decidualize. Coculture of PSC-ESFs with placenta-derived endometrial epithelial cells generated organoids used to examine stromal-epithelial interactions. Cocultures exhibited specific endometrial markers in the appropriate compartments, organization with cell polarity, and hormone responsiveness of both cell types. Furthermore, cocultures recapitulate a central feature of the human decidua by cyclically responding to hormone withdrawal followed by hormone retreatment. This advance enables mechanistic studies of the cyclic responses that characterize the human endometrium. |
| first_indexed | 2024-12-14T22:09:30Z |
| format | Article |
| id | doaj.art-f12da9fe6d524cb6bd0633fc2545a92b |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-14T22:09:30Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-f12da9fe6d524cb6bd0633fc2545a92b2022-12-21T22:45:48ZengElsevierCell Reports2211-12472021-05-01357109138Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human deciduaVirginia Chu Cheung0Chian-Yu Peng1Mirna Marinić2Noboru J. Sakabe3Ivy Aneas4Vincent J. Lynch5Carole Ober6Marcelo A. Nobrega7John A. Kessler8Department of Neurology, Northwestern University, Chicago, IL 60611, USA; Corresponding authorDepartment of Neurology, Northwestern University, Chicago, IL 60611, USADepartment of Human Genetics, University of Chicago, Chicago, IL, USA; Current address: Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL, USADepartment of Human Genetics, University of Chicago, Chicago, IL, USADepartment of Human Genetics, University of Chicago, Chicago, IL, USADepartment of Human Genetics, University of Chicago, Chicago, IL, USA; Department of Biological Sciences, University of Buffalo, Buffalo, NY, USADepartment of Human Genetics, University of Chicago, Chicago, IL, USADepartment of Human Genetics, University of Chicago, Chicago, IL, USADepartment of Neurology, Northwestern University, Chicago, IL 60611, USA; Corresponding authorSummary: Various human diseases and pregnancy-related disorders reflect endometrial dysfunction. However, rodent models do not share fundamental biological processes with the human endometrium, such as spontaneous decidualization, and no existing human cell cultures recapitulate the cyclic interactions between endometrial stromal and epithelial compartments necessary for decidualization and implantation. Here we report a protocol differentiating human pluripotent stem cells into endometrial stromal fibroblasts (PSC-ESFs) that are highly pure and able to decidualize. Coculture of PSC-ESFs with placenta-derived endometrial epithelial cells generated organoids used to examine stromal-epithelial interactions. Cocultures exhibited specific endometrial markers in the appropriate compartments, organization with cell polarity, and hormone responsiveness of both cell types. Furthermore, cocultures recapitulate a central feature of the human decidua by cyclically responding to hormone withdrawal followed by hormone retreatment. This advance enables mechanistic studies of the cyclic responses that characterize the human endometrium.http://www.sciencedirect.com/science/article/pii/S2211124721004770human pluripotent stem cellsendometrial stromal cellsdeciduaepithelial-stromal signalingcyclic hormone responsecell-cell signaling |
| spellingShingle | Virginia Chu Cheung Chian-Yu Peng Mirna Marinić Noboru J. Sakabe Ivy Aneas Vincent J. Lynch Carole Ober Marcelo A. Nobrega John A. Kessler Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua Cell Reports human pluripotent stem cells endometrial stromal cells decidua epithelial-stromal signaling cyclic hormone response cell-cell signaling |
| title | Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua |
| title_full | Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua |
| title_fullStr | Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua |
| title_full_unstemmed | Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua |
| title_short | Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua |
| title_sort | pluripotent stem cell derived endometrial stromal fibroblasts in a cyclic hormone responsive coculture model of human decidua |
| topic | human pluripotent stem cells endometrial stromal cells decidua epithelial-stromal signaling cyclic hormone response cell-cell signaling |
| url | http://www.sciencedirect.com/science/article/pii/S2211124721004770 |
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