Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.)
The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 106 CFU/g). In this context, fi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02243/full |
| _version_ | 1819081199986933760 |
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| author | Alexander Jaramillo-Torres Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield |
| author_facet | Alexander Jaramillo-Torres Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield |
| author_sort | Alexander Jaramillo-Torres |
| collection | DOAJ |
| description | The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 106 CFU/g). In this context, fish health and antiviral response were also investigated. A 12-week feeding trial was conducted in a flow-through rearing system involving 6 weeks in freshwater and 6 weeks in seawater. Fish received a control and probiotic diet. The composition of the salmon gut bacterial communities was determined by high-throughput sequencing of digesta and mucosa samples from both the freshwater and seawater stage. The main phyla detected during both freshwater and seawater stages were Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria. Significant differences were observed between the intestinal microbiota in the digesta and the mucosa. Both probiotic supplementation and the seawater transfer (SWT) had a substantial impact on the microbial communities, with most pronounced changes detected in the mucosal communities after SWT. This last finding together with a significantly higher antiviral response (mx-1 and tlr3 gene expression) in the distal intestine of fish fed the probiotic diet suggest a causal link between the microbiota modulation and activation of antiviral response. Feeding probiotics during the freshwater stage did not significantly increase survival after infectious pancreatic necrosis virus (IPNV) challenge after SWT, although higher survival was observed in one out of two replicate challenge tanks. In conclusion, this study demonstrated that both dietary probiotic supplementation and transfer from freshwater to seawater have an important role in modulating the bacterial communities in the distal intestine of Atlantic salmon. Furthermore, supplementation of the diet with P. acidilactici MA18/5M can modulate antiviral response. |
| first_indexed | 2024-12-21T19:56:59Z |
| format | Article |
| id | doaj.art-f12e02b533624b6db0106cf157b85c37 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T19:56:59Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f12e02b533624b6db0106cf157b85c372022-12-21T18:52:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-09-011010.3389/fmicb.2019.02243477121Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.)Alexander Jaramillo-Torres0Alexander Jaramillo-Torres1Mark D. Rawling2Ana Rodiles3Heidi E. Mikalsen4Lill-Heidi Johansen5John Tinsley6Torunn Forberg7Elisabeth Aasum8Mathieu Castex9Daniel Lee Merrifield10Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, NorwayAquaculture and Fish Nutrition Research Group, School of Biological and Marine Sciences, University of Plymouth, Plymouth, United KingdomAquaculture and Fish Nutrition Research Group, School of Biological and Marine Sciences, University of Plymouth, Plymouth, United KingdomAquaculture and Fish Nutrition Research Group, School of Biological and Marine Sciences, University of Plymouth, Plymouth, United KingdomNofima – Norwegian Institute of Food, Fisheries and Aquaculture Research, Tromsø, NorwayNofima – Norwegian Institute of Food, Fisheries and Aquaculture Research, Tromsø, NorwayBioMar AS, Trondheim, NorwayBioMar AS, Trondheim, NorwayBioMar AS, Trondheim, NorwayLallemand SAS, Blagnac, FranceAquaculture and Fish Nutrition Research Group, School of Biological and Marine Sciences, University of Plymouth, Plymouth, United KingdomThe aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 106 CFU/g). In this context, fish health and antiviral response were also investigated. A 12-week feeding trial was conducted in a flow-through rearing system involving 6 weeks in freshwater and 6 weeks in seawater. Fish received a control and probiotic diet. The composition of the salmon gut bacterial communities was determined by high-throughput sequencing of digesta and mucosa samples from both the freshwater and seawater stage. The main phyla detected during both freshwater and seawater stages were Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria. Significant differences were observed between the intestinal microbiota in the digesta and the mucosa. Both probiotic supplementation and the seawater transfer (SWT) had a substantial impact on the microbial communities, with most pronounced changes detected in the mucosal communities after SWT. This last finding together with a significantly higher antiviral response (mx-1 and tlr3 gene expression) in the distal intestine of fish fed the probiotic diet suggest a causal link between the microbiota modulation and activation of antiviral response. Feeding probiotics during the freshwater stage did not significantly increase survival after infectious pancreatic necrosis virus (IPNV) challenge after SWT, although higher survival was observed in one out of two replicate challenge tanks. In conclusion, this study demonstrated that both dietary probiotic supplementation and transfer from freshwater to seawater have an important role in modulating the bacterial communities in the distal intestine of Atlantic salmon. Furthermore, supplementation of the diet with P. acidilactici MA18/5M can modulate antiviral response.https://www.frontiersin.org/article/10.3389/fmicb.2019.02243/fullfishmicrobiotaintestinePediococcus acidilacticiseawater transferhigh-throughput sequencing |
| spellingShingle | Alexander Jaramillo-Torres Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) Frontiers in Microbiology fish microbiota intestine Pediococcus acidilactici seawater transfer high-throughput sequencing |
| title | Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
| title_full | Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
| title_fullStr | Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
| title_full_unstemmed | Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
| title_short | Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
| title_sort | influence of dietary supplementation of probiotic pediococcus acidilactici ma18 5m during the transition from freshwater to seawater on intestinal health and microbiota of atlantic salmon salmo salar l |
| topic | fish microbiota intestine Pediococcus acidilactici seawater transfer high-throughput sequencing |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02243/full |
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