Verticillium dahliae chromatin remodeling facilitates the DNA damage repair in response to plant ROS stress.
Reactive oxygen species (ROS) production is one of the earliest responses when plants percept pathogens and acts as antimicrobials to block pathogen entry. However, whether and how pathogens tolerate ROS stress remains elusive. Here, we report the chromatin remodeling in Verticillium dahliae, a soil...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-04-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1008481 |
| _version_ | 1818582279882014720 |
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| author | Sheng Wang Xue-Ming Wu Chuan-Hui Liu Jing-Yun Shang Feng Gao Hui-Shan Guo |
| author_facet | Sheng Wang Xue-Ming Wu Chuan-Hui Liu Jing-Yun Shang Feng Gao Hui-Shan Guo |
| author_sort | Sheng Wang |
| collection | DOAJ |
| description | Reactive oxygen species (ROS) production is one of the earliest responses when plants percept pathogens and acts as antimicrobials to block pathogen entry. However, whether and how pathogens tolerate ROS stress remains elusive. Here, we report the chromatin remodeling in Verticillium dahliae, a soil-borne pathogenic fungus that causes vascular wilts of a wide range of plants, facilitates the DNA damage repair in response to plant ROS stress. We identified VdDpb4, encoding a histone-fold protein of the ISW2 chromatin remodeling complex in V. dahliae, is a virulence gene. The reduced virulence in wild type Arabidopsis plants arising from VdDpb4 deletion was impaired in the rbohd mutant plants that did not produce ROS. Further characterization of VdDpb4 and its interacting protein, VdIsw2, an ATP-dependent chromatin-remodeling factor, we show that while the depletion of VdIsw2 led to the decondensing of chromatin, the depletion of VdDpb4 resulted in a more compact chromatin structure and affected the VdIsw2-dependent transcriptional effect on gene expression, including genes involved in DNA damage repair. A knockout mutant of either VdDpb4 or VdIsw2 reduced the efficiency of DNA repair in the presence of DNA-damaging agents and virulence during plant infection. Together, our data demonstrate that VdDpb4 and VdIsw2 play roles in maintaining chromatin structure for positioning nucleosomes and transcription regulation, including genes involved in DNA repair in response to ROS stress during development and plant infection. |
| first_indexed | 2024-12-16T07:46:52Z |
| format | Article |
| id | doaj.art-f1302b1a16a2407ebe4220f2f77543bf |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-16T07:46:52Z |
| publishDate | 2020-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-f1302b1a16a2407ebe4220f2f77543bf2022-12-21T22:38:56ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742020-04-01164e100848110.1371/journal.ppat.1008481Verticillium dahliae chromatin remodeling facilitates the DNA damage repair in response to plant ROS stress.Sheng WangXue-Ming WuChuan-Hui LiuJing-Yun ShangFeng GaoHui-Shan GuoReactive oxygen species (ROS) production is one of the earliest responses when plants percept pathogens and acts as antimicrobials to block pathogen entry. However, whether and how pathogens tolerate ROS stress remains elusive. Here, we report the chromatin remodeling in Verticillium dahliae, a soil-borne pathogenic fungus that causes vascular wilts of a wide range of plants, facilitates the DNA damage repair in response to plant ROS stress. We identified VdDpb4, encoding a histone-fold protein of the ISW2 chromatin remodeling complex in V. dahliae, is a virulence gene. The reduced virulence in wild type Arabidopsis plants arising from VdDpb4 deletion was impaired in the rbohd mutant plants that did not produce ROS. Further characterization of VdDpb4 and its interacting protein, VdIsw2, an ATP-dependent chromatin-remodeling factor, we show that while the depletion of VdIsw2 led to the decondensing of chromatin, the depletion of VdDpb4 resulted in a more compact chromatin structure and affected the VdIsw2-dependent transcriptional effect on gene expression, including genes involved in DNA damage repair. A knockout mutant of either VdDpb4 or VdIsw2 reduced the efficiency of DNA repair in the presence of DNA-damaging agents and virulence during plant infection. Together, our data demonstrate that VdDpb4 and VdIsw2 play roles in maintaining chromatin structure for positioning nucleosomes and transcription regulation, including genes involved in DNA repair in response to ROS stress during development and plant infection.https://doi.org/10.1371/journal.ppat.1008481 |
| spellingShingle | Sheng Wang Xue-Ming Wu Chuan-Hui Liu Jing-Yun Shang Feng Gao Hui-Shan Guo Verticillium dahliae chromatin remodeling facilitates the DNA damage repair in response to plant ROS stress. PLoS Pathogens |
| title | Verticillium dahliae chromatin remodeling facilitates the DNA damage repair in response to plant ROS stress. |
| title_full | Verticillium dahliae chromatin remodeling facilitates the DNA damage repair in response to plant ROS stress. |
| title_fullStr | Verticillium dahliae chromatin remodeling facilitates the DNA damage repair in response to plant ROS stress. |
| title_full_unstemmed | Verticillium dahliae chromatin remodeling facilitates the DNA damage repair in response to plant ROS stress. |
| title_short | Verticillium dahliae chromatin remodeling facilitates the DNA damage repair in response to plant ROS stress. |
| title_sort | verticillium dahliae chromatin remodeling facilitates the dna damage repair in response to plant ros stress |
| url | https://doi.org/10.1371/journal.ppat.1008481 |
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