Solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5°C for up to 120 h
ABSTRACT: Information on prolonging the storage duration of cold semen with acceptable fertility in roosters is limited. This study aimed to determine the efficiency of solid storage with the addition of various concentrations of serine to the Thai native rooster (Pradu Hang Dum) semen extender on s...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123001724 |
| _version_ | 1827941014620340224 |
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| author | Theerapat Kheawkanha Vibuntita Chankitisakul Pachara Thananurak Maruay Pimprasert Wuttigrai Boonkum Thevin Vongpralub |
| author_facet | Theerapat Kheawkanha Vibuntita Chankitisakul Pachara Thananurak Maruay Pimprasert Wuttigrai Boonkum Thevin Vongpralub |
| author_sort | Theerapat Kheawkanha |
| collection | DOAJ |
| description | ABSTRACT: Information on prolonging the storage duration of cold semen with acceptable fertility in roosters is limited. This study aimed to determine the efficiency of solid storage with the addition of various concentrations of serine to the Thai native rooster (Pradu Hang Dum) semen extender on semen quality and fertility potential during storage at 5°C for up to 120 h. Pooled semen was diluted with a base extender and a gelatin extender containing 0, 2, 4, and 6 mM serine, then stored at 5°C for 120 h. In Experiment 1, the semen quality and malondialdehyde (MDA) concentrations were assessed at 0, 24, 72, and 120 h after storage. In Experiment 2, fertility potential in terms of fertility and hatchability rates was determined using the most effective solid-storage semen from Experiment 1. Sperm quality decreased with increasing storage time (P < 0.05). The lowest semen quality was observed in the control group since T24 of storage compared with the other groups (P < 0.05). Progressive motility, viability, and mitochondrial function were higher (P < 0.05) in the extender supplemented with gelatin and serine groups than those in the gelatin alone group at T72 and T120. In the extender supplemented with gelatin and serine groups, the highest semen quality was observed in the gelatin with 4 mM serine groups. The differences among extenders supplemented with serine were insignificant (P > 0.05), and the lowest MDA was observed in the gelatin with 4 mM serine groups. The fertility and hatchability rates in gelatin with 4 mM serine at T24 were comparable to those in fresh semen (83.87 and 86.12% vs. 86.66 and 88.3%; P > 0.05). Those of T72 were significantly better than those of the control at the same hour of storage (64.08 and 71.61% vs. 52.38 and 64.48%), while those of T120 were not different among groups. In summary, a semen extender as a solid medium supplemented with 4 mM serine successfully preserved the rooster semen for a long duration up to 72 h of storage time. |
| first_indexed | 2024-03-13T09:30:44Z |
| format | Article |
| id | doaj.art-f132910a1ba4402c8c2ad73dc662af81 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-13T09:30:44Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-f132910a1ba4402c8c2ad73dc662af812023-05-26T04:20:45ZengElsevierPoultry Science0032-57912023-06-011026102648Solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5°C for up to 120 hTheerapat Kheawkanha0Vibuntita Chankitisakul1Pachara Thananurak2Maruay Pimprasert3Wuttigrai Boonkum4Thevin Vongpralub5Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand; Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen 40002, Thailand; Corresponding author:Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand; Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandABSTRACT: Information on prolonging the storage duration of cold semen with acceptable fertility in roosters is limited. This study aimed to determine the efficiency of solid storage with the addition of various concentrations of serine to the Thai native rooster (Pradu Hang Dum) semen extender on semen quality and fertility potential during storage at 5°C for up to 120 h. Pooled semen was diluted with a base extender and a gelatin extender containing 0, 2, 4, and 6 mM serine, then stored at 5°C for 120 h. In Experiment 1, the semen quality and malondialdehyde (MDA) concentrations were assessed at 0, 24, 72, and 120 h after storage. In Experiment 2, fertility potential in terms of fertility and hatchability rates was determined using the most effective solid-storage semen from Experiment 1. Sperm quality decreased with increasing storage time (P < 0.05). The lowest semen quality was observed in the control group since T24 of storage compared with the other groups (P < 0.05). Progressive motility, viability, and mitochondrial function were higher (P < 0.05) in the extender supplemented with gelatin and serine groups than those in the gelatin alone group at T72 and T120. In the extender supplemented with gelatin and serine groups, the highest semen quality was observed in the gelatin with 4 mM serine groups. The differences among extenders supplemented with serine were insignificant (P > 0.05), and the lowest MDA was observed in the gelatin with 4 mM serine groups. The fertility and hatchability rates in gelatin with 4 mM serine at T24 were comparable to those in fresh semen (83.87 and 86.12% vs. 86.66 and 88.3%; P > 0.05). Those of T72 were significantly better than those of the control at the same hour of storage (64.08 and 71.61% vs. 52.38 and 64.48%), while those of T120 were not different among groups. In summary, a semen extender as a solid medium supplemented with 4 mM serine successfully preserved the rooster semen for a long duration up to 72 h of storage time.http://www.sciencedirect.com/science/article/pii/S0032579123001724long-term storagecooling storagerooster semengelatin |
| spellingShingle | Theerapat Kheawkanha Vibuntita Chankitisakul Pachara Thananurak Maruay Pimprasert Wuttigrai Boonkum Thevin Vongpralub Solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5°C for up to 120 h Poultry Science long-term storage cooling storage rooster semen gelatin |
| title | Solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5°C for up to 120 h |
| title_full | Solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5°C for up to 120 h |
| title_fullStr | Solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5°C for up to 120 h |
| title_full_unstemmed | Solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5°C for up to 120 h |
| title_short | Solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5°C for up to 120 h |
| title_sort | solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5°c for up to 120 h |
| topic | long-term storage cooling storage rooster semen gelatin |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123001724 |
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