A study on combination of daptomycin with selected antimicrobial agents: in vitro synergistic effect of MIC value of 1 mg/L against MRSA strains
Abstract Background Daptomycin is an important drug used in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infection. A high dose of daptomycin is indicated for an MRSA infection with a minimum inhibitory concentration (MIC) of 1 mg/L for daptomycin. Combination therapies with d...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-05-01
|
| Series: | BMC Pharmacology and Toxicology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40360-019-0305-y |
| _version_ | 1818777890542583808 |
|---|---|
| author | Yi-Chien Lee Pao-Yu Chen Jann-Tay Wang Shan-Chwen Chang |
| author_facet | Yi-Chien Lee Pao-Yu Chen Jann-Tay Wang Shan-Chwen Chang |
| author_sort | Yi-Chien Lee |
| collection | DOAJ |
| description | Abstract Background Daptomycin is an important drug used in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infection. A high dose of daptomycin is indicated for an MRSA infection with a minimum inhibitory concentration (MIC) of 1 mg/L for daptomycin. Combination therapies with daptomycin and other antimicrobial agents, including fosfomycin, display in vitro synergism potentially. This study was conducted to investigate the in vitro synergistic effect of daptomycin-based combination therapy against MRSA strains with high daptomycin MIC. Method The synergistic effects of daptomycin in combination with fosfomycin, gentamicin, linezolid, oxacillin, or rifampicin against MRSA with an MIC of 1 mg/L for daptomycin were measured using the microbroth checkerboard assay in vitro. Result A total of 100 MRSA isolates was tested. The synergistic interactions of the drugs were evaluated using the fractional inhibitory concentration index. The MIC values revealed that all isolates (100%) were found to be susceptible to linezolid, 85% to fosfomycin, 8% to gentamicin, 69% to rifampicin, and no isolate was susceptible to oxacillin. The in vitro synergism rates of daptomycin in combination with fosfomycin, oxacillin, gentamicin, linezolid, and rifampicin were 37, 11, 5, 3, and 1%, respectively. Conclusion The combination of daptomycin plus fosfomycin may be an effective therapeutic option for MRSA infection. |
| first_indexed | 2024-12-18T11:36:01Z |
| format | Article |
| id | doaj.art-f134595d57b44d4f9bffd6bd84709014 |
| institution | Directory Open Access Journal |
| issn | 2050-6511 |
| language | English |
| last_indexed | 2024-12-18T11:36:01Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pharmacology and Toxicology |
| spelling | doaj.art-f134595d57b44d4f9bffd6bd847090142022-12-21T21:09:31ZengBMCBMC Pharmacology and Toxicology2050-65112019-05-012011610.1186/s40360-019-0305-yA study on combination of daptomycin with selected antimicrobial agents: in vitro synergistic effect of MIC value of 1 mg/L against MRSA strainsYi-Chien Lee0Pao-Yu Chen1Jann-Tay Wang2Shan-Chwen Chang3Department of Internal Medicine, Fu Jen Catholic University Hospital, Fu Jen Catholic UniversityDepartment of Internal Medicine, National Taiwan University HospitalDepartment of Internal Medicine, National Taiwan University HospitalDepartment of Internal Medicine, National Taiwan University HospitalAbstract Background Daptomycin is an important drug used in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infection. A high dose of daptomycin is indicated for an MRSA infection with a minimum inhibitory concentration (MIC) of 1 mg/L for daptomycin. Combination therapies with daptomycin and other antimicrobial agents, including fosfomycin, display in vitro synergism potentially. This study was conducted to investigate the in vitro synergistic effect of daptomycin-based combination therapy against MRSA strains with high daptomycin MIC. Method The synergistic effects of daptomycin in combination with fosfomycin, gentamicin, linezolid, oxacillin, or rifampicin against MRSA with an MIC of 1 mg/L for daptomycin were measured using the microbroth checkerboard assay in vitro. Result A total of 100 MRSA isolates was tested. The synergistic interactions of the drugs were evaluated using the fractional inhibitory concentration index. The MIC values revealed that all isolates (100%) were found to be susceptible to linezolid, 85% to fosfomycin, 8% to gentamicin, 69% to rifampicin, and no isolate was susceptible to oxacillin. The in vitro synergism rates of daptomycin in combination with fosfomycin, oxacillin, gentamicin, linezolid, and rifampicin were 37, 11, 5, 3, and 1%, respectively. Conclusion The combination of daptomycin plus fosfomycin may be an effective therapeutic option for MRSA infection.http://link.springer.com/article/10.1186/s40360-019-0305-yCombination therapyDaptomycinMRSACheckerboard assays |
| spellingShingle | Yi-Chien Lee Pao-Yu Chen Jann-Tay Wang Shan-Chwen Chang A study on combination of daptomycin with selected antimicrobial agents: in vitro synergistic effect of MIC value of 1 mg/L against MRSA strains BMC Pharmacology and Toxicology Combination therapy Daptomycin MRSA Checkerboard assays |
| title | A study on combination of daptomycin with selected antimicrobial agents: in vitro synergistic effect of MIC value of 1 mg/L against MRSA strains |
| title_full | A study on combination of daptomycin with selected antimicrobial agents: in vitro synergistic effect of MIC value of 1 mg/L against MRSA strains |
| title_fullStr | A study on combination of daptomycin with selected antimicrobial agents: in vitro synergistic effect of MIC value of 1 mg/L against MRSA strains |
| title_full_unstemmed | A study on combination of daptomycin with selected antimicrobial agents: in vitro synergistic effect of MIC value of 1 mg/L against MRSA strains |
| title_short | A study on combination of daptomycin with selected antimicrobial agents: in vitro synergistic effect of MIC value of 1 mg/L against MRSA strains |
| title_sort | study on combination of daptomycin with selected antimicrobial agents in vitro synergistic effect of mic value of 1 mg l against mrsa strains |
| topic | Combination therapy Daptomycin MRSA Checkerboard assays |
| url | http://link.springer.com/article/10.1186/s40360-019-0305-y |
| work_keys_str_mv | AT yichienlee astudyoncombinationofdaptomycinwithselectedantimicrobialagentsinvitrosynergisticeffectofmicvalueof1mglagainstmrsastrains AT paoyuchen astudyoncombinationofdaptomycinwithselectedantimicrobialagentsinvitrosynergisticeffectofmicvalueof1mglagainstmrsastrains AT janntaywang astudyoncombinationofdaptomycinwithselectedantimicrobialagentsinvitrosynergisticeffectofmicvalueof1mglagainstmrsastrains AT shanchwenchang astudyoncombinationofdaptomycinwithselectedantimicrobialagentsinvitrosynergisticeffectofmicvalueof1mglagainstmrsastrains AT yichienlee studyoncombinationofdaptomycinwithselectedantimicrobialagentsinvitrosynergisticeffectofmicvalueof1mglagainstmrsastrains AT paoyuchen studyoncombinationofdaptomycinwithselectedantimicrobialagentsinvitrosynergisticeffectofmicvalueof1mglagainstmrsastrains AT janntaywang studyoncombinationofdaptomycinwithselectedantimicrobialagentsinvitrosynergisticeffectofmicvalueof1mglagainstmrsastrains AT shanchwenchang studyoncombinationofdaptomycinwithselectedantimicrobialagentsinvitrosynergisticeffectofmicvalueof1mglagainstmrsastrains |