Effect of Salt on Gelation Behavior of Injectable Nanocomposite Scaffold Based on Hydroxypropyl Methylcellulose and Hydroxyapatite/Tricalcium Phosphate Nanoparticles
Biocompatible cellulosic polymer hydrogels are used in in-situ forming injectable scaffolds for bone regeneration. The hydrogels, however, generally suffer from their inherent weak mechanical properties. Calcium phosphate particles are used to enhance the mechanical strength and to improve the bone...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Iran Polymer and Petrochemical Institute
2014-06-01
|
| Series: | علوم و تکنولوژی پلیمر |
| Subjects: | |
| Online Access: | http://jips.ippi.ac.ir/article_1034_1075ec5934acb8787474e72783717999.pdf |
| _version_ | 1819152437554970624 |
|---|---|
| author | Roghayyeh Marefat Seyedlar Mohammad Atai Azizollah Nodehi Mohammad Imani |
| author_facet | Roghayyeh Marefat Seyedlar Mohammad Atai Azizollah Nodehi Mohammad Imani |
| author_sort | Roghayyeh Marefat Seyedlar |
| collection | DOAJ |
| description | Biocompatible cellulosic polymer hydrogels are used in in-situ forming injectable scaffolds for bone regeneration. The hydrogels, however, generally suffer from their inherent weak mechanical properties. Calcium phosphate particles are used to enhance the mechanical strength and to improve the bone tissue regeneration capability of the scaffolds. In this study, hydroxypropyl methylcellulose (HPMC) was utilized as a polymeric matrix and β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAp) nanoparticles were used to improve the mechanical properties. β-TCP Nanoparticles with plate-like morphology was synthesized through a wet chemical precipitation method. The morphology of the particles was confirmed using scanning electron microscope (SEM). Because the gelation temperature of the HPMC solution was above the temperature of normal human body, different concentrations of sodium sulphate (Na2SO4) were incorporated into the HPMC solutions to examine and adjust the gelation temperature. The cloud point and gelation temperature of the hydrogels were measured using UV/Vis spectroscopy and oscillatory rheometry, respectively. The injectability of the hydrogels, with different inorganic contents, was also measured using a universal testing machine. The results indicated that the cloud point and the gelation temperature of the hydrogels dropped with increase in the sodium sulphate concentration due to Hofmeister effect. The rheology measurements also revealed that β-tricalcium phosphate was more effective than hydroxyapatite in reducing the gelation temperature and enhancement of the modulus and viscosity of the hydrogels. The HPMC hydrogels containing β-tricalcium phosphate and hydroxyapatite nanoparticles were injectable at room temperature. The hydrogels based on HPMC matrix and the calcium phosphate nanoparticles provided promising hydrogels applicable as in-situ forming injectable scaffolds. |
| first_indexed | 2024-12-22T14:49:17Z |
| format | Article |
| id | doaj.art-f1354f4336704180b0429061e04005ac |
| institution | Directory Open Access Journal |
| issn | 1016-3255 2008-0883 |
| language | fas |
| last_indexed | 2024-12-22T14:49:17Z |
| publishDate | 2014-06-01 |
| publisher | Iran Polymer and Petrochemical Institute |
| record_format | Article |
| series | علوم و تکنولوژی پلیمر |
| spelling | doaj.art-f1354f4336704180b0429061e04005ac2022-12-21T18:22:22ZfasIran Polymer and Petrochemical Instituteعلوم و تکنولوژی پلیمر1016-32552008-08832014-06-012721099910.22063/jipst.2014.10341034Effect of Salt on Gelation Behavior of Injectable Nanocomposite Scaffold Based on Hydroxypropyl Methylcellulose and Hydroxyapatite/Tricalcium Phosphate NanoparticlesRoghayyeh Marefat Seyedlar0Mohammad Atai1Azizollah Nodehi2Mohammad Imani3Faculty of Science, Iran Polymer and Petrochemical Institute, P.O. Box: 14975-112, Tehran, IranFaculty of Science, Iran Polymer and Petrochemical Institute, P.O. Box: 14975-112, Tehran, IranFaculty of Science, Iran Polymer and Petrochemical Institute, P.O. Box: 14975-112, Tehran, IranFaculty of Science, Iran Polymer and Petrochemical Institute, P.O. Box: 14975-112, Tehran, IranBiocompatible cellulosic polymer hydrogels are used in in-situ forming injectable scaffolds for bone regeneration. The hydrogels, however, generally suffer from their inherent weak mechanical properties. Calcium phosphate particles are used to enhance the mechanical strength and to improve the bone tissue regeneration capability of the scaffolds. In this study, hydroxypropyl methylcellulose (HPMC) was utilized as a polymeric matrix and β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAp) nanoparticles were used to improve the mechanical properties. β-TCP Nanoparticles with plate-like morphology was synthesized through a wet chemical precipitation method. The morphology of the particles was confirmed using scanning electron microscope (SEM). Because the gelation temperature of the HPMC solution was above the temperature of normal human body, different concentrations of sodium sulphate (Na2SO4) were incorporated into the HPMC solutions to examine and adjust the gelation temperature. The cloud point and gelation temperature of the hydrogels were measured using UV/Vis spectroscopy and oscillatory rheometry, respectively. The injectability of the hydrogels, with different inorganic contents, was also measured using a universal testing machine. The results indicated that the cloud point and the gelation temperature of the hydrogels dropped with increase in the sodium sulphate concentration due to Hofmeister effect. The rheology measurements also revealed that β-tricalcium phosphate was more effective than hydroxyapatite in reducing the gelation temperature and enhancement of the modulus and viscosity of the hydrogels. The HPMC hydrogels containing β-tricalcium phosphate and hydroxyapatite nanoparticles were injectable at room temperature. The hydrogels based on HPMC matrix and the calcium phosphate nanoparticles provided promising hydrogels applicable as in-situ forming injectable scaffolds.http://jips.ippi.ac.ir/article_1034_1075ec5934acb8787474e72783717999.pdfcalcium phosphate nanoparticlesinjectable scaffoldgelationcloud pointhydroxypropyl methylcellulose |
| spellingShingle | Roghayyeh Marefat Seyedlar Mohammad Atai Azizollah Nodehi Mohammad Imani Effect of Salt on Gelation Behavior of Injectable Nanocomposite Scaffold Based on Hydroxypropyl Methylcellulose and Hydroxyapatite/Tricalcium Phosphate Nanoparticles علوم و تکنولوژی پلیمر calcium phosphate nanoparticles injectable scaffold gelation cloud point hydroxypropyl methylcellulose |
| title | Effect of Salt on Gelation Behavior of Injectable Nanocomposite Scaffold Based on Hydroxypropyl Methylcellulose and Hydroxyapatite/Tricalcium Phosphate Nanoparticles |
| title_full | Effect of Salt on Gelation Behavior of Injectable Nanocomposite Scaffold Based on Hydroxypropyl Methylcellulose and Hydroxyapatite/Tricalcium Phosphate Nanoparticles |
| title_fullStr | Effect of Salt on Gelation Behavior of Injectable Nanocomposite Scaffold Based on Hydroxypropyl Methylcellulose and Hydroxyapatite/Tricalcium Phosphate Nanoparticles |
| title_full_unstemmed | Effect of Salt on Gelation Behavior of Injectable Nanocomposite Scaffold Based on Hydroxypropyl Methylcellulose and Hydroxyapatite/Tricalcium Phosphate Nanoparticles |
| title_short | Effect of Salt on Gelation Behavior of Injectable Nanocomposite Scaffold Based on Hydroxypropyl Methylcellulose and Hydroxyapatite/Tricalcium Phosphate Nanoparticles |
| title_sort | effect of salt on gelation behavior of injectable nanocomposite scaffold based on hydroxypropyl methylcellulose and hydroxyapatite tricalcium phosphate nanoparticles |
| topic | calcium phosphate nanoparticles injectable scaffold gelation cloud point hydroxypropyl methylcellulose |
| url | http://jips.ippi.ac.ir/article_1034_1075ec5934acb8787474e72783717999.pdf |
| work_keys_str_mv | AT roghayyehmarefatseyedlar effectofsaltongelationbehaviorofinjectablenanocompositescaffoldbasedonhydroxypropylmethylcelluloseandhydroxyapatitetricalciumphosphatenanoparticles AT mohammadatai effectofsaltongelationbehaviorofinjectablenanocompositescaffoldbasedonhydroxypropylmethylcelluloseandhydroxyapatitetricalciumphosphatenanoparticles AT azizollahnodehi effectofsaltongelationbehaviorofinjectablenanocompositescaffoldbasedonhydroxypropylmethylcelluloseandhydroxyapatitetricalciumphosphatenanoparticles AT mohammadimani effectofsaltongelationbehaviorofinjectablenanocompositescaffoldbasedonhydroxypropylmethylcelluloseandhydroxyapatitetricalciumphosphatenanoparticles |