Effect of inhibiting miR-155 on apoptosis and inflammation reaction in myocardial ischemia-reperfusion model
Objective To explore the effect of inhibiting miR-155 on cardiomyocyte apoptosis and inflammation in myocardial ischemia-reperfusion (MI/R) model. Methods Myocardial cells of H9c2 rats were divided into four groups: blank control group, myocardial ischemia-reperfusion group (MI/R group), nega...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-12-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210041.pdf |
| _version_ | 1827390559114756096 |
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| author | ZHU Xin-hua, LIU Bei-bei, HOU Jing-wen, LI Qiu-ying, XU Hui-juan, ZHANG Xiao-yang |
| author_facet | ZHU Xin-hua, LIU Bei-bei, HOU Jing-wen, LI Qiu-ying, XU Hui-juan, ZHANG Xiao-yang |
| author_sort | ZHU Xin-hua, LIU Bei-bei, HOU Jing-wen, LI Qiu-ying, XU Hui-juan, ZHANG Xiao-yang |
| collection | DOAJ |
| description | Objective To explore the effect of inhibiting miR-155 on cardiomyocyte apoptosis and inflammation in myocardial ischemia-reperfusion (MI/R) model. Methods Myocardial cells of H9c2 rats were divided into four groups: blank control group, myocardial ischemia-reperfusion group (MI/R group), negative control group and miR-155 inhibitor group. The expressions of miR-155, NF-κB signaling pathway related genes and Bax, Bcl-2 mRNA were detected by RT-qPCR. The contents of IL-8 and TNF-α were detected by ELISA. Western blot was used to detect the expression of NF-κB signaling pathway related genes and Bax, Bcl-2 protein. Results Compared with the control group, the expression of miR-155 in MI/R group was significantly increased (P＜0.05) while the expression of miR-155 in miR-155 inhibitor group was significantly decreased (P＜0.05). In MI/R group, the apoptosis rate, the content of IL-8 and TNF-α, the protein and mRNA expression of Bax and NF-κB signal pathway were significantly higher than those in the control group, while the protein and mRNA expression of Bcl-2 were lower (P＜0.05). After inhibition the expression of miR-155, the apoptosis rate, the content of IL-8 and TNF-α, the protein and mRNA expression level of Bax and NF-κB signal pathway related gene were significantly decreased, while the protein and mRNA expression by Bcl-2 were increased (P＜0.05). Conclusions Inhibition of miR-155 expression can protect MI/R injury through inhibition of apoptosis and inflammation. The potential mechanism is the inhibition of the activation of NF-κB signaling pathway. |
| first_indexed | 2024-03-08T16:54:27Z |
| format | Article |
| id | doaj.art-f137da68e88546abba599ef84bbc982a |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:27Z |
| publishDate | 2021-12-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-f137da68e88546abba599ef84bbc982a2024-01-05T03:07:37ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-12-01411217861791Effect of inhibiting miR-155 on apoptosis and inflammation reaction in myocardial ischemia-reperfusion modelZHU Xin-hua, LIU Bei-bei, HOU Jing-wen, LI Qiu-ying, XU Hui-juan, ZHANG Xiao-yang0Department of Geriatrics, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, ChinaObjective To explore the effect of inhibiting miR-155 on cardiomyocyte apoptosis and inflammation in myocardial ischemia-reperfusion (MI/R) model. Methods Myocardial cells of H9c2 rats were divided into four groups: blank control group, myocardial ischemia-reperfusion group (MI/R group), negative control group and miR-155 inhibitor group. The expressions of miR-155, NF-κB signaling pathway related genes and Bax, Bcl-2 mRNA were detected by RT-qPCR. The contents of IL-8 and TNF-α were detected by ELISA. Western blot was used to detect the expression of NF-κB signaling pathway related genes and Bax, Bcl-2 protein. Results Compared with the control group, the expression of miR-155 in MI/R group was significantly increased (P＜0.05) while the expression of miR-155 in miR-155 inhibitor group was significantly decreased (P＜0.05). In MI/R group, the apoptosis rate, the content of IL-8 and TNF-α, the protein and mRNA expression of Bax and NF-κB signal pathway were significantly higher than those in the control group, while the protein and mRNA expression of Bcl-2 were lower (P＜0.05). After inhibition the expression of miR-155, the apoptosis rate, the content of IL-8 and TNF-α, the protein and mRNA expression level of Bax and NF-κB signal pathway related gene were significantly decreased, while the protein and mRNA expression by Bcl-2 were increased (P＜0.05). Conclusions Inhibition of miR-155 expression can protect MI/R injury through inhibition of apoptosis and inflammation. The potential mechanism is the inhibition of the activation of NF-κB signaling pathway.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210041.pdfmir-155|myocardial ischemia-reperfusion|apoptosis|inflammatory|nf-κb signaling pathway |
| spellingShingle | ZHU Xin-hua, LIU Bei-bei, HOU Jing-wen, LI Qiu-ying, XU Hui-juan, ZHANG Xiao-yang Effect of inhibiting miR-155 on apoptosis and inflammation reaction in myocardial ischemia-reperfusion model Jichu yixue yu linchuang mir-155|myocardial ischemia-reperfusion|apoptosis|inflammatory|nf-κb signaling pathway |
| title | Effect of inhibiting miR-155 on apoptosis and inflammation reaction in myocardial ischemia-reperfusion model |
| title_full | Effect of inhibiting miR-155 on apoptosis and inflammation reaction in myocardial ischemia-reperfusion model |
| title_fullStr | Effect of inhibiting miR-155 on apoptosis and inflammation reaction in myocardial ischemia-reperfusion model |
| title_full_unstemmed | Effect of inhibiting miR-155 on apoptosis and inflammation reaction in myocardial ischemia-reperfusion model |
| title_short | Effect of inhibiting miR-155 on apoptosis and inflammation reaction in myocardial ischemia-reperfusion model |
| title_sort | effect of inhibiting mir 155 on apoptosis and inflammation reaction in myocardial ischemia reperfusion model |
| topic | mir-155|myocardial ischemia-reperfusion|apoptosis|inflammatory|nf-κb signaling pathway |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210041.pdf |
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