TaqMan Assays for Simultaneous Detection of <i>Bacillus anthracis</i> and <i>Bacillus cereus</i> biovar <i>anthracis</i>
Anthrax is a worldwide zoonotic disease caused by the spore-forming bacterium <i>Bacillus anthracis</i>. Primarily a disease of herbivores, human infections often result from direct contact with contaminated animal products (cutaneous and inhalational anthrax) or through consumption of i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/12/1074 |
| _version_ | 1797544035948167168 |
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| author | Diansy Zincke Michael H. Norris Odalis Cruz Berzhan Kurmanov W. Scott McGraw David J. Daegling John Krigbaum Thi Thu Ha Hoang Kamil Khanipov Georgiy Golovko Ted Hadfield Jason K. Blackburn |
| author_facet | Diansy Zincke Michael H. Norris Odalis Cruz Berzhan Kurmanov W. Scott McGraw David J. Daegling John Krigbaum Thi Thu Ha Hoang Kamil Khanipov Georgiy Golovko Ted Hadfield Jason K. Blackburn |
| author_sort | Diansy Zincke |
| collection | DOAJ |
| description | Anthrax is a worldwide zoonotic disease caused by the spore-forming bacterium <i>Bacillus anthracis</i>. Primarily a disease of herbivores, human infections often result from direct contact with contaminated animal products (cutaneous and inhalational anthrax) or through consumption of infected meat (gastrointestinal anthrax). The genetic near neighbor, <i>Bacillus cereus</i> biovar <i>anthracis</i> (Bcbva), causes an anthrax-like illness in the wildlife and livestock of west and central Africa due to the presence and expression of <i>B. anthracis</i>-specific virulence factors in this background. While Bcbva infections have not been reported in humans, a recent seroprevalence study detected Bcbva antibodies in the rural population around Taï National Park. This work describes the development of new TaqMan multiplex PCRs for the simultaneous detection of <i>B. anthracis</i> and Bcbva. The assays are designed to amplify Ba-1, <i>capB</i>, and <i>lef</i> markers in <i>B. anthracis</i> and genomic island IV (GI4), <i>capB</i>, and <i>lef</i> in Bcbva. Our assays allow for the rapid discrimination of <i>B. anthracis</i> and Bcbva and will provide insights into the molecular epidemiology of these two important pathogens that share an overlapping geographical range in west and central Africa. |
| first_indexed | 2024-03-10T13:53:48Z |
| format | Article |
| id | doaj.art-f13a3374777445b9834c29635d2b142a |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T13:53:48Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-f13a3374777445b9834c29635d2b142a2023-11-21T01:53:39ZengMDPI AGPathogens2076-08172020-12-01912107410.3390/pathogens9121074TaqMan Assays for Simultaneous Detection of <i>Bacillus anthracis</i> and <i>Bacillus cereus</i> biovar <i>anthracis</i>Diansy Zincke0Michael H. Norris1Odalis Cruz2Berzhan Kurmanov3W. Scott McGraw4David J. Daegling5John Krigbaum6Thi Thu Ha Hoang7Kamil Khanipov8Georgiy Golovko9Ted Hadfield10Jason K. Blackburn11Spatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida, Gainesville, FL 32611, USASpatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida, Gainesville, FL 32611, USASpatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida, Gainesville, FL 32611, USASpatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida, Gainesville, FL 32611, USADepartment of Anthropology, The Ohio State University, Columbus, OH 43210, USADepartment of Anthropology, University of Florida, Gainesville, FL 32611, USADepartment of Anthropology, University of Florida, Gainesville, FL 32611, USANational Institute of Hygiene and Epidemiology, Hanoi 10000, VietnamDepartment of Pharmacology & Toxicology, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Pharmacology & Toxicology, University of Texas Medical Branch, Galveston, TX 77555, USASpatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida, Gainesville, FL 32611, USASpatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida, Gainesville, FL 32611, USAAnthrax is a worldwide zoonotic disease caused by the spore-forming bacterium <i>Bacillus anthracis</i>. Primarily a disease of herbivores, human infections often result from direct contact with contaminated animal products (cutaneous and inhalational anthrax) or through consumption of infected meat (gastrointestinal anthrax). The genetic near neighbor, <i>Bacillus cereus</i> biovar <i>anthracis</i> (Bcbva), causes an anthrax-like illness in the wildlife and livestock of west and central Africa due to the presence and expression of <i>B. anthracis</i>-specific virulence factors in this background. While Bcbva infections have not been reported in humans, a recent seroprevalence study detected Bcbva antibodies in the rural population around Taï National Park. This work describes the development of new TaqMan multiplex PCRs for the simultaneous detection of <i>B. anthracis</i> and Bcbva. The assays are designed to amplify Ba-1, <i>capB</i>, and <i>lef</i> markers in <i>B. anthracis</i> and genomic island IV (GI4), <i>capB</i>, and <i>lef</i> in Bcbva. Our assays allow for the rapid discrimination of <i>B. anthracis</i> and Bcbva and will provide insights into the molecular epidemiology of these two important pathogens that share an overlapping geographical range in west and central Africa.https://www.mdpi.com/2076-0817/9/12/1074<i>Bacillus anthracis</i><i>Bacillus cereus</i> biovar <i>anthracis</i>anthraxmultiplexBa-1Africa |
| spellingShingle | Diansy Zincke Michael H. Norris Odalis Cruz Berzhan Kurmanov W. Scott McGraw David J. Daegling John Krigbaum Thi Thu Ha Hoang Kamil Khanipov Georgiy Golovko Ted Hadfield Jason K. Blackburn TaqMan Assays for Simultaneous Detection of <i>Bacillus anthracis</i> and <i>Bacillus cereus</i> biovar <i>anthracis</i> Pathogens <i>Bacillus anthracis</i> <i>Bacillus cereus</i> biovar <i>anthracis</i> anthrax multiplex Ba-1 Africa |
| title | TaqMan Assays for Simultaneous Detection of <i>Bacillus anthracis</i> and <i>Bacillus cereus</i> biovar <i>anthracis</i> |
| title_full | TaqMan Assays for Simultaneous Detection of <i>Bacillus anthracis</i> and <i>Bacillus cereus</i> biovar <i>anthracis</i> |
| title_fullStr | TaqMan Assays for Simultaneous Detection of <i>Bacillus anthracis</i> and <i>Bacillus cereus</i> biovar <i>anthracis</i> |
| title_full_unstemmed | TaqMan Assays for Simultaneous Detection of <i>Bacillus anthracis</i> and <i>Bacillus cereus</i> biovar <i>anthracis</i> |
| title_short | TaqMan Assays for Simultaneous Detection of <i>Bacillus anthracis</i> and <i>Bacillus cereus</i> biovar <i>anthracis</i> |
| title_sort | taqman assays for simultaneous detection of i bacillus anthracis i and i bacillus cereus i biovar i anthracis i |
| topic | <i>Bacillus anthracis</i> <i>Bacillus cereus</i> biovar <i>anthracis</i> anthrax multiplex Ba-1 Africa |
| url | https://www.mdpi.com/2076-0817/9/12/1074 |
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