Diagnostic value and role of serum miR-15a-5p in patients with schizophrenia
Abstract Background More and more studies have confirmed that the heredity plays an important role in mental disorders, especially microRNA. The objective of this research was to explore the level of miR-15a-5p in patients with schizophrenia (SZ), and to evaluate the feasibility of this miRNA as a d...
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BMC
2024-01-01
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Series: | Annals of General Psychiatry |
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Online Access: | https://doi.org/10.1186/s12991-023-00489-4 |
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author | Zhen Xu Ruidong Yang Guanwen Chen Mingjun Jiang |
author_facet | Zhen Xu Ruidong Yang Guanwen Chen Mingjun Jiang |
author_sort | Zhen Xu |
collection | DOAJ |
description | Abstract Background More and more studies have confirmed that the heredity plays an important role in mental disorders, especially microRNA. The objective of this research was to explore the level of miR-15a-5p in patients with schizophrenia (SZ), and to evaluate the feasibility of this miRNA as a diagnostic marker of SZ. Methods The serum level of miR-15a-5p in patients with SZ and healthy people was detected by RT-qPCR. ROC curve was established to evaluate the clinical diagnostic significance of miR-15a-5p in SZ. Pearson correlation coefficient was used to evaluate the correlation between miR-15a-5p level and PANSS score. Logistic regression was used to assess the risk factors of SZ. A rat model of SZ was established, and the effects of miR-15a-5p on the behavior of SZ rats were observed through water maze test and open field test. Results The serum level of miR-15a-5p in patients with SZ was significantly increased, and ROC analysis revealed that miR-15a-5p had clinical diagnostic value in SZ. High level of miR-15a-5p was positively correlated with the positive symptom, negative symptom and general psychopathology subscore of patients. Logistic regression results showed that miR-15a-5p was a risk factor affecting the occurrence of SZ. Animal studies showed that the serum level of miR-15a-5p was elevated in the SZ rats, and inhibiting the expression of miR-15a-5p has a positive effect on improving the cognitive function and anxiety behavior of SZ rats. Conclusions Serum miR-15a-5p is a risk factor for SZ, which is of great significance for the diagnosis of SZ. |
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id | doaj.art-f13bd26387784374a28e2f1f5a3656d5 |
institution | Directory Open Access Journal |
issn | 1744-859X |
language | English |
last_indexed | 2024-03-08T16:15:48Z |
publishDate | 2024-01-01 |
publisher | BMC |
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series | Annals of General Psychiatry |
spelling | doaj.art-f13bd26387784374a28e2f1f5a3656d52024-01-07T12:38:10ZengBMCAnnals of General Psychiatry1744-859X2024-01-0123111010.1186/s12991-023-00489-4Diagnostic value and role of serum miR-15a-5p in patients with schizophreniaZhen Xu0Ruidong Yang1Guanwen Chen2Mingjun Jiang3Department of Anesthesiology, The Eighth Medical Center of PLA General HospitalDepartment of Adult Cardiovascular Surgery, The Sixth Medical Center of PLA General HospitalGuangdong Nantian Institute of Forensic ScienceShenzhen Polytechnic UniversityAbstract Background More and more studies have confirmed that the heredity plays an important role in mental disorders, especially microRNA. The objective of this research was to explore the level of miR-15a-5p in patients with schizophrenia (SZ), and to evaluate the feasibility of this miRNA as a diagnostic marker of SZ. Methods The serum level of miR-15a-5p in patients with SZ and healthy people was detected by RT-qPCR. ROC curve was established to evaluate the clinical diagnostic significance of miR-15a-5p in SZ. Pearson correlation coefficient was used to evaluate the correlation between miR-15a-5p level and PANSS score. Logistic regression was used to assess the risk factors of SZ. A rat model of SZ was established, and the effects of miR-15a-5p on the behavior of SZ rats were observed through water maze test and open field test. Results The serum level of miR-15a-5p in patients with SZ was significantly increased, and ROC analysis revealed that miR-15a-5p had clinical diagnostic value in SZ. High level of miR-15a-5p was positively correlated with the positive symptom, negative symptom and general psychopathology subscore of patients. Logistic regression results showed that miR-15a-5p was a risk factor affecting the occurrence of SZ. Animal studies showed that the serum level of miR-15a-5p was elevated in the SZ rats, and inhibiting the expression of miR-15a-5p has a positive effect on improving the cognitive function and anxiety behavior of SZ rats. Conclusions Serum miR-15a-5p is a risk factor for SZ, which is of great significance for the diagnosis of SZ.https://doi.org/10.1186/s12991-023-00489-4SchizophreniaMiR-15a-5pPANSSMK-801 |
spellingShingle | Zhen Xu Ruidong Yang Guanwen Chen Mingjun Jiang Diagnostic value and role of serum miR-15a-5p in patients with schizophrenia Annals of General Psychiatry Schizophrenia MiR-15a-5p PANSS MK-801 |
title | Diagnostic value and role of serum miR-15a-5p in patients with schizophrenia |
title_full | Diagnostic value and role of serum miR-15a-5p in patients with schizophrenia |
title_fullStr | Diagnostic value and role of serum miR-15a-5p in patients with schizophrenia |
title_full_unstemmed | Diagnostic value and role of serum miR-15a-5p in patients with schizophrenia |
title_short | Diagnostic value and role of serum miR-15a-5p in patients with schizophrenia |
title_sort | diagnostic value and role of serum mir 15a 5p in patients with schizophrenia |
topic | Schizophrenia MiR-15a-5p PANSS MK-801 |
url | https://doi.org/10.1186/s12991-023-00489-4 |
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