Perstraction of Intracellular Pigments through Submerged Fermentation of Talaromyces spp. in a Surfactant Rich Media: A Novel Approach for Enhanced Pigment Recovery
A high percentage of the pigments produced by Talaromyces spp. remains inside the cell, which could lead to a high product concentration inhibition. To overcome this issue an extractive fermentation process, perstraction, was suggested, which involves the extraction of the intracellular products out...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-06-01
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| Series: | Journal of Fungi |
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| Online Access: | http://www.mdpi.com/2309-608X/3/3/33 |
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| author | Lourdes Morales-Oyervides Jorge Oliveira Maria Sousa-Gallagher Alejandro Méndez-Zavala Julio Cesar Montañez |
| author_facet | Lourdes Morales-Oyervides Jorge Oliveira Maria Sousa-Gallagher Alejandro Méndez-Zavala Julio Cesar Montañez |
| author_sort | Lourdes Morales-Oyervides |
| collection | DOAJ |
| description | A high percentage of the pigments produced by Talaromyces spp. remains inside the cell, which could lead to a high product concentration inhibition. To overcome this issue an extractive fermentation process, perstraction, was suggested, which involves the extraction of the intracellular products out of the cell by using a two-phase system during the fermentation. The present work studied the effect of various surfactants on secretion of intracellular pigments produced by Talaromyces spp. in submerged fermentation. Surfactants used were: non-ionic surfactants (Tween 80, Span 20 and Triton X-100) and a polyethylene glycerol polymer 8000, at different concentrations (5, 20, 35 g/L). The highest extracellular pigment yield (16 OD500nm) was reached using Triton X-100 (35 g/L), which was 44% higher than the control (no surfactant added). The effect of addition time of the selected surfactant was further studied. The highest extracellular pigment concentration (22 OD500nm) was achieved when the surfactant was added at 120 h of fermentation. Kinetics of extracellular and intracellular pigments were examined. Total pigment at the end of the fermentation using Triton X-100 was 27.7% higher than the control, confirming that the use of surfactants partially alleviated the product inhibition during the pigment production culture. |
| first_indexed | 2024-12-23T06:54:44Z |
| format | Article |
| id | doaj.art-f13f43a7b27140949a0e04906cf6a5ef |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-12-23T06:54:44Z |
| publishDate | 2017-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-f13f43a7b27140949a0e04906cf6a5ef2022-12-21T17:56:21ZengMDPI AGJournal of Fungi2309-608X2017-06-01333310.3390/jof3030033jof3030033Perstraction of Intracellular Pigments through Submerged Fermentation of Talaromyces spp. in a Surfactant Rich Media: A Novel Approach for Enhanced Pigment RecoveryLourdes Morales-Oyervides0Jorge Oliveira1Maria Sousa-Gallagher2Alejandro Méndez-Zavala3Julio Cesar Montañez4School of Engineering, University College Cork, Cork, IrelandSchool of Engineering, University College Cork, Cork, IrelandSchool of Engineering, University College Cork, Cork, IrelandDepartment of Chemical Engineering, Universidad Autónoma de Coahuila, Saltillo 25280, MexicoDepartment of Chemical Engineering, Universidad Autónoma de Coahuila, Saltillo 25280, MexicoA high percentage of the pigments produced by Talaromyces spp. remains inside the cell, which could lead to a high product concentration inhibition. To overcome this issue an extractive fermentation process, perstraction, was suggested, which involves the extraction of the intracellular products out of the cell by using a two-phase system during the fermentation. The present work studied the effect of various surfactants on secretion of intracellular pigments produced by Talaromyces spp. in submerged fermentation. Surfactants used were: non-ionic surfactants (Tween 80, Span 20 and Triton X-100) and a polyethylene glycerol polymer 8000, at different concentrations (5, 20, 35 g/L). The highest extracellular pigment yield (16 OD500nm) was reached using Triton X-100 (35 g/L), which was 44% higher than the control (no surfactant added). The effect of addition time of the selected surfactant was further studied. The highest extracellular pigment concentration (22 OD500nm) was achieved when the surfactant was added at 120 h of fermentation. Kinetics of extracellular and intracellular pigments were examined. Total pigment at the end of the fermentation using Triton X-100 was 27.7% higher than the control, confirming that the use of surfactants partially alleviated the product inhibition during the pigment production culture.http://www.mdpi.com/2309-608X/3/3/33fungal pigmentsperstractionsurfactantTalaromyces |
| spellingShingle | Lourdes Morales-Oyervides Jorge Oliveira Maria Sousa-Gallagher Alejandro Méndez-Zavala Julio Cesar Montañez Perstraction of Intracellular Pigments through Submerged Fermentation of Talaromyces spp. in a Surfactant Rich Media: A Novel Approach for Enhanced Pigment Recovery Journal of Fungi fungal pigments perstraction surfactant Talaromyces |
| title | Perstraction of Intracellular Pigments through Submerged Fermentation of Talaromyces spp. in a Surfactant Rich Media: A Novel Approach for Enhanced Pigment Recovery |
| title_full | Perstraction of Intracellular Pigments through Submerged Fermentation of Talaromyces spp. in a Surfactant Rich Media: A Novel Approach for Enhanced Pigment Recovery |
| title_fullStr | Perstraction of Intracellular Pigments through Submerged Fermentation of Talaromyces spp. in a Surfactant Rich Media: A Novel Approach for Enhanced Pigment Recovery |
| title_full_unstemmed | Perstraction of Intracellular Pigments through Submerged Fermentation of Talaromyces spp. in a Surfactant Rich Media: A Novel Approach for Enhanced Pigment Recovery |
| title_short | Perstraction of Intracellular Pigments through Submerged Fermentation of Talaromyces spp. in a Surfactant Rich Media: A Novel Approach for Enhanced Pigment Recovery |
| title_sort | perstraction of intracellular pigments through submerged fermentation of talaromyces spp in a surfactant rich media a novel approach for enhanced pigment recovery |
| topic | fungal pigments perstraction surfactant Talaromyces |
| url | http://www.mdpi.com/2309-608X/3/3/33 |
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