SARS‐CoV‐2 antigen detection by saliva; an alternative to nasopharyngeal specimen: A cross‐sectional study
Abstract Background and Aims Saliva samples are less invasive and more convenient for patients than naso‐ and/or oropharynx swabs (NOS). However, there is no US Food and Drug Administration‐approved severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) rapid antigen test kit, which can be use...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | Health Science Reports |
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| Online Access: | https://doi.org/10.1002/hsr2.1275 |
| _version_ | 1797816765381607424 |
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| author | Mohammad Jubair Sezanur Rahman Mst. Noorjahan Begum Muhammad Talha Raisha Musarrat A. K. M. Shafiqur Rahman Mohammed Shehab Uddin AiMin Wen YaoHui Ning Kamrun Nahar Mohammed Ziaur Rahman Mustafizur Rahman |
| author_facet | Mohammad Jubair Sezanur Rahman Mst. Noorjahan Begum Muhammad Talha Raisha Musarrat A. K. M. Shafiqur Rahman Mohammed Shehab Uddin AiMin Wen YaoHui Ning Kamrun Nahar Mohammed Ziaur Rahman Mustafizur Rahman |
| author_sort | Mohammad Jubair |
| collection | DOAJ |
| description | Abstract Background and Aims Saliva samples are less invasive and more convenient for patients than naso‐ and/or oropharynx swabs (NOS). However, there is no US Food and Drug Administration‐approved severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) rapid antigen test kit, which can be useful in a prolonged pandemic to reduce transmission by allowing suspected individuals to self‐sampling. We evaluated the performances of High sensitive AQ+ Rapid SARS‐CoV‐2 Antigen Test (AQ+ kit) using nasopharyngeal swabs (NPs) and saliva specimens from the same patients in laboratory conditions. Methods The real‐time reverse transcription‐polymerase chain reaction (rRT‐PCR) test result was used for screening the inrolled individuals and compared as the gold standard. NP and saliva samples were collected from 100 rRT‐PCR positives and 100 negative individuals and tested with an AQ+ kit. Results The AQ+ kit showed good performances in both NP and saliva samples with an overall accuracy of 98.5% and 94.0%, and sensitivity of 97.0% and 88.0%, respectively. In both cases, specificity was 100%. AQ+ kit performance with saliva was in the range of the World Health Organization recommended value. Conclusion xOur findings indicate that the saliva specimen can be used as an alternative and less invasive to NPs for quick and reliable SARS‐CoV‐2 antigen detection. |
| first_indexed | 2024-03-13T08:42:33Z |
| format | Article |
| id | doaj.art-f13fb26f4fe84cd98323f931d6152acc |
| institution | Directory Open Access Journal |
| issn | 2398-8835 |
| language | English |
| last_indexed | 2024-03-13T08:42:33Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Health Science Reports |
| spelling | doaj.art-f13fb26f4fe84cd98323f931d6152acc2023-05-30T08:58:34ZengWileyHealth Science Reports2398-88352023-05-0165n/an/a10.1002/hsr2.1275SARS‐CoV‐2 antigen detection by saliva; an alternative to nasopharyngeal specimen: A cross‐sectional studyMohammad Jubair0Sezanur Rahman1Mst. Noorjahan Begum2Muhammad Talha3Raisha Musarrat4A. K. M. Shafiqur Rahman5Mohammed Shehab Uddin6AiMin Wen7YaoHui Ning8Kamrun Nahar9Mohammed Ziaur Rahman10Mustafizur Rahman11Virology Laboratory, Infectious Diseases Division icddr,b: International Centre for Diarrhoeal Disease Research, Bangladesh Dhaka BangladeshVirology Laboratory, Infectious Diseases Division icddr,b: International Centre for Diarrhoeal Disease Research, Bangladesh Dhaka BangladeshVirology Laboratory, Infectious Diseases Division icddr,b: International Centre for Diarrhoeal Disease Research, Bangladesh Dhaka BangladeshVirology Laboratory, Infectious Diseases Division icddr,b: International Centre for Diarrhoeal Disease Research, Bangladesh Dhaka BangladeshVirology Laboratory, Infectious Diseases Division icddr,b: International Centre for Diarrhoeal Disease Research, Bangladesh Dhaka BangladeshCOVID Unit DNCC Dedicated Covid‐19 Hospital Dhaka BangladeshCOVID Unit Kuwait Bangladesh Friendship Government Hospital Dhaka BangladeshR&D InTec PRODUCTS, INC Fujian ChinaR&D InTec PRODUCTS, INC Fujian ChinaVirology Laboratory, Infectious Diseases Division icddr,b: International Centre for Diarrhoeal Disease Research, Bangladesh Dhaka BangladeshVirology Laboratory, Infectious Diseases Division icddr,b: International Centre for Diarrhoeal Disease Research, Bangladesh Dhaka BangladeshVirology Laboratory, Infectious Diseases Division icddr,b: International Centre for Diarrhoeal Disease Research, Bangladesh Dhaka BangladeshAbstract Background and Aims Saliva samples are less invasive and more convenient for patients than naso‐ and/or oropharynx swabs (NOS). However, there is no US Food and Drug Administration‐approved severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) rapid antigen test kit, which can be useful in a prolonged pandemic to reduce transmission by allowing suspected individuals to self‐sampling. We evaluated the performances of High sensitive AQ+ Rapid SARS‐CoV‐2 Antigen Test (AQ+ kit) using nasopharyngeal swabs (NPs) and saliva specimens from the same patients in laboratory conditions. Methods The real‐time reverse transcription‐polymerase chain reaction (rRT‐PCR) test result was used for screening the inrolled individuals and compared as the gold standard. NP and saliva samples were collected from 100 rRT‐PCR positives and 100 negative individuals and tested with an AQ+ kit. Results The AQ+ kit showed good performances in both NP and saliva samples with an overall accuracy of 98.5% and 94.0%, and sensitivity of 97.0% and 88.0%, respectively. In both cases, specificity was 100%. AQ+ kit performance with saliva was in the range of the World Health Organization recommended value. Conclusion xOur findings indicate that the saliva specimen can be used as an alternative and less invasive to NPs for quick and reliable SARS‐CoV‐2 antigen detection.https://doi.org/10.1002/hsr2.1275COVID‐19 diagnosiskit evaluationrapid antigen testsalivaSARS‐CoV‐2 |
| spellingShingle | Mohammad Jubair Sezanur Rahman Mst. Noorjahan Begum Muhammad Talha Raisha Musarrat A. K. M. Shafiqur Rahman Mohammed Shehab Uddin AiMin Wen YaoHui Ning Kamrun Nahar Mohammed Ziaur Rahman Mustafizur Rahman SARS‐CoV‐2 antigen detection by saliva; an alternative to nasopharyngeal specimen: A cross‐sectional study Health Science Reports COVID‐19 diagnosis kit evaluation rapid antigen test saliva SARS‐CoV‐2 |
| title | SARS‐CoV‐2 antigen detection by saliva; an alternative to nasopharyngeal specimen: A cross‐sectional study |
| title_full | SARS‐CoV‐2 antigen detection by saliva; an alternative to nasopharyngeal specimen: A cross‐sectional study |
| title_fullStr | SARS‐CoV‐2 antigen detection by saliva; an alternative to nasopharyngeal specimen: A cross‐sectional study |
| title_full_unstemmed | SARS‐CoV‐2 antigen detection by saliva; an alternative to nasopharyngeal specimen: A cross‐sectional study |
| title_short | SARS‐CoV‐2 antigen detection by saliva; an alternative to nasopharyngeal specimen: A cross‐sectional study |
| title_sort | sars cov 2 antigen detection by saliva an alternative to nasopharyngeal specimen a cross sectional study |
| topic | COVID‐19 diagnosis kit evaluation rapid antigen test saliva SARS‐CoV‐2 |
| url | https://doi.org/10.1002/hsr2.1275 |
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