High-Pressure Water Jet System Treatment of Argan Nut Shell and Enzymatic Hydrolysis for Bioethanol Production

Argan nut shell represents the most generated by-product during the process of the extraction of argan oil. For the first time, argan nut shell was characterized and assessed as a new potential feedstock for bioethanol production using a combination of mechanical and enzymatic pretreatment. Argan sh...

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Main Authors: Jihane Zeghlouli, Gwendoline Christophe, Kota Ogura, Naoki Sawamura, Amine Guendouz, Cherkaoui El Modafar, Philippe Michaud, Cédric Delattre
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:Fermentation
Subjects:
Online Access:https://www.mdpi.com/2311-5637/8/11/627
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author Jihane Zeghlouli
Gwendoline Christophe
Kota Ogura
Naoki Sawamura
Amine Guendouz
Cherkaoui El Modafar
Philippe Michaud
Cédric Delattre
author_facet Jihane Zeghlouli
Gwendoline Christophe
Kota Ogura
Naoki Sawamura
Amine Guendouz
Cherkaoui El Modafar
Philippe Michaud
Cédric Delattre
author_sort Jihane Zeghlouli
collection DOAJ
description Argan nut shell represents the most generated by-product during the process of the extraction of argan oil. For the first time, argan nut shell was characterized and assessed as a new potential feedstock for bioethanol production using a combination of mechanical and enzymatic pretreatment. Argan shell samples were first disintegrated using the Star Burst system, which involves a high-pressure water jet system. Then, the pretreated argan nut shell was subjected to enzymatic hydrolysis using Viscozyme L (30 FBGU/g). Afterwards, the fermentation of the hydrolysate by <i>Saccharomyces cerevisiae</i> was investigated. Argan nut shell, as a feedstock plentiful in carbohydrates, conferred a high yield of saccharification (90%) and an optimal ethanol bioconversion (45.25%) using Viscozyme L (30 FBGU/g) at 2%<i><sub>w</sub></i><sub>/<i>v</i></sub> of argan feedstock.
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spelling doaj.art-f13fb5957586476f9a35b5c033c839fb2023-11-24T08:19:33ZengMDPI AGFermentation2311-56372022-11-0181162710.3390/fermentation8110627High-Pressure Water Jet System Treatment of Argan Nut Shell and Enzymatic Hydrolysis for Bioethanol ProductionJihane Zeghlouli0Gwendoline Christophe1Kota Ogura2Naoki Sawamura3Amine Guendouz4Cherkaoui El Modafar5Philippe Michaud6Cédric Delattre7Centre d’Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech, URL-CNRST 05), Faculté des Sciences et Techniques Marrakech, Université Cadi Ayyad, Marrakesh 40000, MoroccoClermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, 63000 Clermont-Ferrand, FranceSugino Machine Limited, 2880 Kuriyama, Namerikawa City 936-8577, JapanSugino Europe GmbH, Mergenthalerallee 77, D-65760 Eschborn, GermanyCentre d’Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech, URL-CNRST 05), Faculté des Sciences et Techniques Marrakech, Université Cadi Ayyad, Marrakesh 40000, MoroccoCentre d’Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech, URL-CNRST 05), Faculté des Sciences et Techniques Marrakech, Université Cadi Ayyad, Marrakesh 40000, MoroccoClermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, 63000 Clermont-Ferrand, FranceClermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, 63000 Clermont-Ferrand, FranceArgan nut shell represents the most generated by-product during the process of the extraction of argan oil. For the first time, argan nut shell was characterized and assessed as a new potential feedstock for bioethanol production using a combination of mechanical and enzymatic pretreatment. Argan shell samples were first disintegrated using the Star Burst system, which involves a high-pressure water jet system. Then, the pretreated argan nut shell was subjected to enzymatic hydrolysis using Viscozyme L (30 FBGU/g). Afterwards, the fermentation of the hydrolysate by <i>Saccharomyces cerevisiae</i> was investigated. Argan nut shell, as a feedstock plentiful in carbohydrates, conferred a high yield of saccharification (90%) and an optimal ethanol bioconversion (45.25%) using Viscozyme L (30 FBGU/g) at 2%<i><sub>w</sub></i><sub>/<i>v</i></sub> of argan feedstock.https://www.mdpi.com/2311-5637/8/11/627argan nut shellmechanical treatmenthigh-pressure water jet systemenzymatic hydrolysisfermentationbioethanol
spellingShingle Jihane Zeghlouli
Gwendoline Christophe
Kota Ogura
Naoki Sawamura
Amine Guendouz
Cherkaoui El Modafar
Philippe Michaud
Cédric Delattre
High-Pressure Water Jet System Treatment of Argan Nut Shell and Enzymatic Hydrolysis for Bioethanol Production
Fermentation
argan nut shell
mechanical treatment
high-pressure water jet system
enzymatic hydrolysis
fermentation
bioethanol
title High-Pressure Water Jet System Treatment of Argan Nut Shell and Enzymatic Hydrolysis for Bioethanol Production
title_full High-Pressure Water Jet System Treatment of Argan Nut Shell and Enzymatic Hydrolysis for Bioethanol Production
title_fullStr High-Pressure Water Jet System Treatment of Argan Nut Shell and Enzymatic Hydrolysis for Bioethanol Production
title_full_unstemmed High-Pressure Water Jet System Treatment of Argan Nut Shell and Enzymatic Hydrolysis for Bioethanol Production
title_short High-Pressure Water Jet System Treatment of Argan Nut Shell and Enzymatic Hydrolysis for Bioethanol Production
title_sort high pressure water jet system treatment of argan nut shell and enzymatic hydrolysis for bioethanol production
topic argan nut shell
mechanical treatment
high-pressure water jet system
enzymatic hydrolysis
fermentation
bioethanol
url https://www.mdpi.com/2311-5637/8/11/627
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AT kotaogura highpressurewaterjetsystemtreatmentofargannutshellandenzymatichydrolysisforbioethanolproduction
AT naokisawamura highpressurewaterjetsystemtreatmentofargannutshellandenzymatichydrolysisforbioethanolproduction
AT amineguendouz highpressurewaterjetsystemtreatmentofargannutshellandenzymatichydrolysisforbioethanolproduction
AT cherkaouielmodafar highpressurewaterjetsystemtreatmentofargannutshellandenzymatichydrolysisforbioethanolproduction
AT philippemichaud highpressurewaterjetsystemtreatmentofargannutshellandenzymatichydrolysisforbioethanolproduction
AT cedricdelattre highpressurewaterjetsystemtreatmentofargannutshellandenzymatichydrolysisforbioethanolproduction