Identification of Mycobacterium Tuberculosis Complex Infection in Human By Indirect ELISA Using Low Molecular Weight Proteins from Culture Filtrate of Mtb Strain C
Background and purpose: Tuberculosis is one of the major global health problems with high mortality. Establishing a rapid and low-cost identification method is significant, especially in developing countries. In this study, we aimed to design an indirect ELISA system with low molecular weight antige...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2022-11-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-18329-en.html |
| _version_ | 1797893191430569984 |
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| author | Mozhgan Khosrobeygi Nader Mosavari Mitra Salehi Naheed Mojgani Majid Akbari |
| author_facet | Mozhgan Khosrobeygi Nader Mosavari Mitra Salehi Naheed Mojgani Majid Akbari |
| author_sort | Mozhgan Khosrobeygi |
| collection | DOAJ |
| description | Background and purpose: Tuberculosis is one of the major global health problems with high mortality. Establishing a rapid and low-cost identification method is significant, especially in developing countries. In this study, we aimed to design an indirect ELISA system with low molecular weight antigens secreted by Mycobacterium tuberculosis strain C, for identification of Mycobacterium tuberculosis Complex infection in human.
Materials and methods: The secretory proteins from culture filtrate of Mtb strain C extracted by Sephadex-G50 were used as target antigen in designing an indirect ELISA system. Then, human sera with tuberculin skin test, culture, acid-fast bacilli smear, and PCR test results were selected to detect human serum antibodies against these low molecular weight proteins.
Results: During gel chromatography and SDS-PAGE (12.5%) analysis, the purified protein F2 fractions with approximately 0.7 mg/ml and molecular weights in the range of 14-40 kDa were used as the target antigen in designing the indirect ELISA system. The ELISA system designed by F2 showed 78% sensitivity and 92% specificity in identifying tuberculosis.
Conclusion: The ELISA results based on low molecular weight proteins, with the sensitivity and specificity achieved in this study, might support the hypothesis that the Mtb culture filtrate antigens could be used as a rapid and sensitive assay in ELISA test for detection of pulmonary TB. |
| first_indexed | 2024-04-10T06:48:56Z |
| format | Article |
| id | doaj.art-f14068457df8400289948bc7b5979597 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T06:48:56Z |
| publishDate | 2022-11-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-f14068457df8400289948bc7b59795972023-02-28T09:09:01ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792022-11-013221490101Identification of Mycobacterium Tuberculosis Complex Infection in Human By Indirect ELISA Using Low Molecular Weight Proteins from Culture Filtrate of Mtb Strain CMozhgan Khosrobeygi0Nader Mosavari1Mitra Salehi2Naheed Mojgani3Majid Akbari4 PhD Candidate in Microbiology, Department of Biological Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran Associate Professor, Department of Tuberculin and Mallein Production and Research, Razi Vaccine and Serum Research Institute, Karaj, Iran Assistant Professor, Department of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran Assistant Professor, Department of Tuberculin and Mallein Production and Research, Razi Vaccine and Serum Research Institute, Alborz, Iran Assistant Professor, Department of Microbiology, Arak University of Medical Sciences, Arak, Iran Background and purpose: Tuberculosis is one of the major global health problems with high mortality. Establishing a rapid and low-cost identification method is significant, especially in developing countries. In this study, we aimed to design an indirect ELISA system with low molecular weight antigens secreted by Mycobacterium tuberculosis strain C, for identification of Mycobacterium tuberculosis Complex infection in human. Materials and methods: The secretory proteins from culture filtrate of Mtb strain C extracted by Sephadex-G50 were used as target antigen in designing an indirect ELISA system. Then, human sera with tuberculin skin test, culture, acid-fast bacilli smear, and PCR test results were selected to detect human serum antibodies against these low molecular weight proteins. Results: During gel chromatography and SDS-PAGE (12.5%) analysis, the purified protein F2 fractions with approximately 0.7 mg/ml and molecular weights in the range of 14-40 kDa were used as the target antigen in designing the indirect ELISA system. The ELISA system designed by F2 showed 78% sensitivity and 92% specificity in identifying tuberculosis. Conclusion: The ELISA results based on low molecular weight proteins, with the sensitivity and specificity achieved in this study, might support the hypothesis that the Mtb culture filtrate antigens could be used as a rapid and sensitive assay in ELISA test for detection of pulmonary TB.http://jmums.mazums.ac.ir/article-1-18329-en.htmlmycobacterium tuberculosislow-molecular weight proteinssds-pageindirect elisa |
| spellingShingle | Mozhgan Khosrobeygi Nader Mosavari Mitra Salehi Naheed Mojgani Majid Akbari Identification of Mycobacterium Tuberculosis Complex Infection in Human By Indirect ELISA Using Low Molecular Weight Proteins from Culture Filtrate of Mtb Strain C Journal of Mazandaran University of Medical Sciences mycobacterium tuberculosis low-molecular weight proteins sds-page indirect elisa |
| title | Identification of Mycobacterium Tuberculosis Complex Infection in Human By Indirect ELISA Using Low Molecular Weight Proteins from Culture Filtrate of Mtb Strain C |
| title_full | Identification of Mycobacterium Tuberculosis Complex Infection in Human By Indirect ELISA Using Low Molecular Weight Proteins from Culture Filtrate of Mtb Strain C |
| title_fullStr | Identification of Mycobacterium Tuberculosis Complex Infection in Human By Indirect ELISA Using Low Molecular Weight Proteins from Culture Filtrate of Mtb Strain C |
| title_full_unstemmed | Identification of Mycobacterium Tuberculosis Complex Infection in Human By Indirect ELISA Using Low Molecular Weight Proteins from Culture Filtrate of Mtb Strain C |
| title_short | Identification of Mycobacterium Tuberculosis Complex Infection in Human By Indirect ELISA Using Low Molecular Weight Proteins from Culture Filtrate of Mtb Strain C |
| title_sort | identification of mycobacterium tuberculosis complex infection in human by indirect elisa using low molecular weight proteins from culture filtrate of mtb strain c |
| topic | mycobacterium tuberculosis low-molecular weight proteins sds-page indirect elisa |
| url | http://jmums.mazums.ac.ir/article-1-18329-en.html |
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