Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection
Summary: We report the follow-up laboratory investigation of three MPXV cases infected in May-June 2022 from diagnosis to disease resolution, monitoring viral shedding in different body fluids and antibody kinetics. Out of 138 non-lesion samples, viral DNA was found in 92.3% saliva, 85.7% semen, 86....
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223001797 |
| _version_ | 1797924735054512128 |
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| author | Francesca Colavita Valentina Mazzotta Gabriella Rozera Isabella Abbate Fabrizio Carletti Carmela Pinnetti Giulia Matusali Silvia Meschi Annalisa Mondi Daniele Lapa Serena Vita Claudia Minosse Camilla Aguglia Roberta Gagliardini Eliana Specchiarello Aurora Bettini Emanuele Nicastri Enrico Girardi Francesco Vaia Andrea Antinori Fabrizio Maggi |
| author_facet | Francesca Colavita Valentina Mazzotta Gabriella Rozera Isabella Abbate Fabrizio Carletti Carmela Pinnetti Giulia Matusali Silvia Meschi Annalisa Mondi Daniele Lapa Serena Vita Claudia Minosse Camilla Aguglia Roberta Gagliardini Eliana Specchiarello Aurora Bettini Emanuele Nicastri Enrico Girardi Francesco Vaia Andrea Antinori Fabrizio Maggi |
| author_sort | Francesca Colavita |
| collection | DOAJ |
| description | Summary: We report the follow-up laboratory investigation of three MPXV cases infected in May-June 2022 from diagnosis to disease resolution, monitoring viral shedding in different body fluids and antibody kinetics. Out of 138 non-lesion samples, viral DNA was found in 92.3% saliva, 85.7% semen, 86.2% oropharyngeal swabs, 51.7% plasma, 46.1% stool, and 9.5% urine samples. Viral load quantified by digital PCR widely varied, but tend to be higher in oropharyngeal swabs, saliva, and stool. Replication competent virus was recovered from four out of seventeen samples, including 1 saliva, 1 oropharyngeal swabs, 1 semen, and 1 stool. The analysis of the antibody kinetics revealed that IgM, IgA, and IgG antibodies were detected within two weeks post-symptoms onset for all three patients, with IgG detected early on at day 4-8 and IgM and IgA showing lower titers along the time frame of the study. Antibody levels increased during the second week of illness with IgG reaching high titers. |
| first_indexed | 2024-04-10T15:05:52Z |
| format | Article |
| id | doaj.art-f14181313809438cb69442a3fa934f24 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T15:05:52Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-f14181313809438cb69442a3fa934f242023-02-15T04:28:38ZengElsevieriScience2589-00422023-03-01263106102Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infectionFrancesca Colavita0Valentina Mazzotta1Gabriella Rozera2Isabella Abbate3Fabrizio Carletti4Carmela Pinnetti5Giulia Matusali6Silvia Meschi7Annalisa Mondi8Daniele Lapa9Serena Vita10Claudia Minosse11Camilla Aguglia12Roberta Gagliardini13Eliana Specchiarello14Aurora Bettini15Emanuele Nicastri16Enrico Girardi17Francesco Vaia18Andrea Antinori19Fabrizio Maggi20Laboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyClinical and Research Infectious Diseases Department, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyLaboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyLaboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyLaboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyClinical and Research Infectious Diseases Department, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyLaboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, Italy; Corresponding authorLaboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyClinical and Research Infectious Diseases Department, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyLaboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyClinical and Research Infectious Diseases Department, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyLaboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyClinical and Research Infectious Diseases Department, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyClinical and Research Infectious Diseases Department, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyLaboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyLaboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyClinical and Research Infectious Diseases Department, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyScientific Direction, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyGeneral Direction, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyClinical and Research Infectious Diseases Department, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, ItalyLaboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, Italy; Corresponding authorSummary: We report the follow-up laboratory investigation of three MPXV cases infected in May-June 2022 from diagnosis to disease resolution, monitoring viral shedding in different body fluids and antibody kinetics. Out of 138 non-lesion samples, viral DNA was found in 92.3% saliva, 85.7% semen, 86.2% oropharyngeal swabs, 51.7% plasma, 46.1% stool, and 9.5% urine samples. Viral load quantified by digital PCR widely varied, but tend to be higher in oropharyngeal swabs, saliva, and stool. Replication competent virus was recovered from four out of seventeen samples, including 1 saliva, 1 oropharyngeal swabs, 1 semen, and 1 stool. The analysis of the antibody kinetics revealed that IgM, IgA, and IgG antibodies were detected within two weeks post-symptoms onset for all three patients, with IgG detected early on at day 4-8 and IgM and IgA showing lower titers along the time frame of the study. Antibody levels increased during the second week of illness with IgG reaching high titers.http://www.sciencedirect.com/science/article/pii/S2589004223001797ImmunologyVirology |
| spellingShingle | Francesca Colavita Valentina Mazzotta Gabriella Rozera Isabella Abbate Fabrizio Carletti Carmela Pinnetti Giulia Matusali Silvia Meschi Annalisa Mondi Daniele Lapa Serena Vita Claudia Minosse Camilla Aguglia Roberta Gagliardini Eliana Specchiarello Aurora Bettini Emanuele Nicastri Enrico Girardi Francesco Vaia Andrea Antinori Fabrizio Maggi Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection iScience Immunology Virology |
| title | Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection |
| title_full | Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection |
| title_fullStr | Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection |
| title_full_unstemmed | Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection |
| title_short | Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection |
| title_sort | kinetics of viral dna in body fluids and antibody response in patients with acute monkeypox virus infection |
| topic | Immunology Virology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223001797 |
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