Composition, functionality and structural correlates of mixed lipid monolayers at air-water interface
Physicochemical properties of lipid monolayer depend on its composition where a blend of lipids exhibit superior behaviour than the individual component. Surface pressure (π) – area (A) isotherms of mixed monolayer (PCmix) formed by dipalmitoylphosphatidylcholine (DPPC), palmitoleylphosphatidylcholi...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | JCIS Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666934X21000210 |
| _version_ | 1819242109949968384 |
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| author | Emili Manna Manas Barai Manas Kumar Mandal Habiba Sultana Alexey G. Bykov Alexander V. Akentiev Boris A. Noskov Shin-ichi Yusa Amiya Kumar Panda |
| author_facet | Emili Manna Manas Barai Manas Kumar Mandal Habiba Sultana Alexey G. Bykov Alexander V. Akentiev Boris A. Noskov Shin-ichi Yusa Amiya Kumar Panda |
| author_sort | Emili Manna |
| collection | DOAJ |
| description | Physicochemical properties of lipid monolayer depend on its composition where a blend of lipids exhibit superior behaviour than the individual component. Surface pressure (π) – area (A) isotherms of mixed monolayer (PCmix) formed by dipalmitoylphosphatidylcholine (DPPC), palmitoleylphosphatidylcholine (POPC), soyphosphatidylcholine (SPC) and hydrogenated soy phosphatidylcholine (HSPC) in combination with 30 mole % cholesterol (Chol) were obtained by using Langmuir trough. Effects of dipalmitoylphosphatidyethanolamine (DPPE) on its mutual miscibility at the air-water interface with DPPC + Chol, POPC + Chol, HSPC + Chol and SPC + Chol were also investigated separately, followed by studying the effects of DPPE and dipalmitoylphosphatidylglycerol (DPPG) to PCmix + Chol and PCmix + DPPE + Chol respectively. Lift-off area, minimum molecular area, excess molecular area, collapse pressure, Gibbs free energy of interfacial mixing, compressibility modulii values were evaluated by analyzing the isotherms. Deviations from the ideal mixing behaviours were dependent on the composition of the lipid blends. Surface dilatational rheology studies could assess monolayer elasticity, whereas the film morphologies were analysed by Brewster angle microscopic (BAM) studies. DPPC induced formation of condensed monolayer, whereas film rigidity was increased with the incorporation of DPPE and DPPG into mixed systems. Interactions among the lipid components of the investigated mixed systems were thoroughly discussed from the point of view of polar head and acyl chain saturation and obtained results follow the sequence: PCmix + DPPE + DPPG + Chol > PCmix + DPPE + Chol > individual PC + DPPE + Chol which could be translated into the bilayer studies. Such investigation of mixed lipid is important for class of its own composition and combined results are expected to contribute in better understanding the interaction of selected lipid in proposed blend. |
| first_indexed | 2024-12-23T14:34:35Z |
| format | Article |
| id | doaj.art-f1435c5226ff42689fa82a4a7f2cf4e6 |
| institution | Directory Open Access Journal |
| issn | 2666-934X |
| language | English |
| last_indexed | 2024-12-23T14:34:35Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JCIS Open |
| spelling | doaj.art-f1435c5226ff42689fa82a4a7f2cf4e62022-12-21T17:43:24ZengElsevierJCIS Open2666-934X2021-10-013100022Composition, functionality and structural correlates of mixed lipid monolayers at air-water interfaceEmili Manna0Manas Barai1Manas Kumar Mandal2Habiba Sultana3Alexey G. Bykov4Alexander V. Akentiev5Boris A. Noskov6Shin-ichi Yusa7Amiya Kumar Panda8Centre for Life Sciences, Vidyasagar University, Midnapore-721102, West Bengal, India; Department of Chemistry, Vidyasagar University, Midnapore-721102, West Bengal, IndiaDepartment of Chemistry, Vidyasagar University, Midnapore-721102, West Bengal, IndiaDepartment of Chemistry, Vidyasagar University, Midnapore-721102, West Bengal, IndiaDepartment of Chemistry, Vidyasagar University, Midnapore-721102, West Bengal, IndiaDepartment of Colloid Chemistry, Saint Petersburg State University, Universiteskii pr.26, 198504 Saint Petersburg, RussiaDepartment of Colloid Chemistry, Saint Petersburg State University, Universiteskii pr.26, 198504 Saint Petersburg, RussiaDepartment of Colloid Chemistry, Saint Petersburg State University, Universiteskii pr.26, 198504 Saint Petersburg, RussiaDepartment of Applied Chemistry, Graduate School of Engineering,University of Hyogo, Hyogo 671-2280, JapanDepartment of Chemistry, Vidyasagar University, Midnapore-721102, West Bengal, India; Corresponding author.Physicochemical properties of lipid monolayer depend on its composition where a blend of lipids exhibit superior behaviour than the individual component. Surface pressure (π) – area (A) isotherms of mixed monolayer (PCmix) formed by dipalmitoylphosphatidylcholine (DPPC), palmitoleylphosphatidylcholine (POPC), soyphosphatidylcholine (SPC) and hydrogenated soy phosphatidylcholine (HSPC) in combination with 30 mole % cholesterol (Chol) were obtained by using Langmuir trough. Effects of dipalmitoylphosphatidyethanolamine (DPPE) on its mutual miscibility at the air-water interface with DPPC + Chol, POPC + Chol, HSPC + Chol and SPC + Chol were also investigated separately, followed by studying the effects of DPPE and dipalmitoylphosphatidylglycerol (DPPG) to PCmix + Chol and PCmix + DPPE + Chol respectively. Lift-off area, minimum molecular area, excess molecular area, collapse pressure, Gibbs free energy of interfacial mixing, compressibility modulii values were evaluated by analyzing the isotherms. Deviations from the ideal mixing behaviours were dependent on the composition of the lipid blends. Surface dilatational rheology studies could assess monolayer elasticity, whereas the film morphologies were analysed by Brewster angle microscopic (BAM) studies. DPPC induced formation of condensed monolayer, whereas film rigidity was increased with the incorporation of DPPE and DPPG into mixed systems. Interactions among the lipid components of the investigated mixed systems were thoroughly discussed from the point of view of polar head and acyl chain saturation and obtained results follow the sequence: PCmix + DPPE + DPPG + Chol > PCmix + DPPE + Chol > individual PC + DPPE + Chol which could be translated into the bilayer studies. Such investigation of mixed lipid is important for class of its own composition and combined results are expected to contribute in better understanding the interaction of selected lipid in proposed blend.http://www.sciencedirect.com/science/article/pii/S2666934X21000210PhospholipidsLangmuir troughMonolayerMembraneSurface rheologyBAM |
| spellingShingle | Emili Manna Manas Barai Manas Kumar Mandal Habiba Sultana Alexey G. Bykov Alexander V. Akentiev Boris A. Noskov Shin-ichi Yusa Amiya Kumar Panda Composition, functionality and structural correlates of mixed lipid monolayers at air-water interface JCIS Open Phospholipids Langmuir trough Monolayer Membrane Surface rheology BAM |
| title | Composition, functionality and structural correlates of mixed lipid monolayers at air-water interface |
| title_full | Composition, functionality and structural correlates of mixed lipid monolayers at air-water interface |
| title_fullStr | Composition, functionality and structural correlates of mixed lipid monolayers at air-water interface |
| title_full_unstemmed | Composition, functionality and structural correlates of mixed lipid monolayers at air-water interface |
| title_short | Composition, functionality and structural correlates of mixed lipid monolayers at air-water interface |
| title_sort | composition functionality and structural correlates of mixed lipid monolayers at air water interface |
| topic | Phospholipids Langmuir trough Monolayer Membrane Surface rheology BAM |
| url | http://www.sciencedirect.com/science/article/pii/S2666934X21000210 |
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