A Functional Single Nucleotide Polymorphism in the 3′ Untranslated Region of the Porcine JARID2 Gene Is Associated with Aggressive Behavior of Weaned Pigs after Mixing
In pig production, pigs often show more aggressive behavior after mixing, which adversely affects animal welfare and growth performance. The Jumonji and structural domain-rich AT interaction domain 2 (JARID2) gene plays an important role in neurodevelopment in mice and various psychiatric disorders...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/1/27 |
| _version_ | 1797358766497202176 |
|---|---|
| author | Huan Yang Chunlei Zhang Xiaohuan Chao Jing Zhao Mingzheng Liu Jiahao Chen Shuhan Liu Tianshuo Wang Asim Muhammad Allan P. Schinckel Bo Zhou |
| author_facet | Huan Yang Chunlei Zhang Xiaohuan Chao Jing Zhao Mingzheng Liu Jiahao Chen Shuhan Liu Tianshuo Wang Asim Muhammad Allan P. Schinckel Bo Zhou |
| author_sort | Huan Yang |
| collection | DOAJ |
| description | In pig production, pigs often show more aggressive behavior after mixing, which adversely affects animal welfare and growth performance. The Jumonji and structural domain-rich AT interaction domain 2 (JARID2) gene plays an important role in neurodevelopment in mice and various psychiatric disorders in humans. The JARID2 gene may impact the aggressive behavior of pigs. By observing the behavior of 500 weaned pigs during the first 72 h after mixing, the ear tissue samples of the 12 most aggressive and 12 least aggressive pigs were selected for DNA resequencing based on the intensity of their aggressive behavior. Large group correlation analysis indicated that the rs3262221458 site located in the 3′-UTR region of the porcine JARID2 gene has a strong relationship with the aggressive behavior of weaned pigs. Pigs with the mutant TT genotype of rs3262221458 have more aggressive behavior than those pigs with the GG and GT genotypes. The dual luciferase assay indicated that the luciferase activity of the plasmids containing the G allele of rs326221458 was significantly less than that of plasmids containing the T allele of rs326221458 and control groups. The binding ability of miR-9828-3p to sequences containing the T allele was less than that of sequences containing the G allele. The overexpression of miR-9828-3p in porcine neuroglial cells (PNGCs) and PK15 cells significantly decreased the mRNA and protein levels of the JARID2 gene. In addition, miR-9828-3p inhibited the proliferation of PNGCs. After inhibiting miR-9828-3p, the mRNA and protein expression levels of JARID2 increased, and the proliferation of PNGCs showed an opposite trend to the cells that forced the expression of miR-9828-3p. In addition, interference with the JARID2 gene by siRNA can effectively inhibit the proliferation of PNGCs. In summary, we found that the rs326221458 locus regulates the expression of the JARID2 gene by affecting the binding of miR-9828-3p and the JARID2 gene, thereby affecting the aggressive behavior of weaned pigs after mixing. |
| first_indexed | 2024-03-08T15:06:54Z |
| format | Article |
| id | doaj.art-f143fd6875c94dea84311ec547e25d77 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:06:54Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f143fd6875c94dea84311ec547e25d772024-01-10T14:57:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-012512710.3390/ijms25010027A Functional Single Nucleotide Polymorphism in the 3′ Untranslated Region of the Porcine JARID2 Gene Is Associated with Aggressive Behavior of Weaned Pigs after MixingHuan Yang0Chunlei Zhang1Xiaohuan Chao2Jing Zhao3Mingzheng Liu4Jiahao Chen5Shuhan Liu6Tianshuo Wang7Asim Muhammad8Allan P. Schinckel9Bo Zhou10College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Sciences, Purdue University, West Lafayette, IN 47907, USACollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaIn pig production, pigs often show more aggressive behavior after mixing, which adversely affects animal welfare and growth performance. The Jumonji and structural domain-rich AT interaction domain 2 (JARID2) gene plays an important role in neurodevelopment in mice and various psychiatric disorders in humans. The JARID2 gene may impact the aggressive behavior of pigs. By observing the behavior of 500 weaned pigs during the first 72 h after mixing, the ear tissue samples of the 12 most aggressive and 12 least aggressive pigs were selected for DNA resequencing based on the intensity of their aggressive behavior. Large group correlation analysis indicated that the rs3262221458 site located in the 3′-UTR region of the porcine JARID2 gene has a strong relationship with the aggressive behavior of weaned pigs. Pigs with the mutant TT genotype of rs3262221458 have more aggressive behavior than those pigs with the GG and GT genotypes. The dual luciferase assay indicated that the luciferase activity of the plasmids containing the G allele of rs326221458 was significantly less than that of plasmids containing the T allele of rs326221458 and control groups. The binding ability of miR-9828-3p to sequences containing the T allele was less than that of sequences containing the G allele. The overexpression of miR-9828-3p in porcine neuroglial cells (PNGCs) and PK15 cells significantly decreased the mRNA and protein levels of the JARID2 gene. In addition, miR-9828-3p inhibited the proliferation of PNGCs. After inhibiting miR-9828-3p, the mRNA and protein expression levels of JARID2 increased, and the proliferation of PNGCs showed an opposite trend to the cells that forced the expression of miR-9828-3p. In addition, interference with the JARID2 gene by siRNA can effectively inhibit the proliferation of PNGCs. In summary, we found that the rs326221458 locus regulates the expression of the JARID2 gene by affecting the binding of miR-9828-3p and the JARID2 gene, thereby affecting the aggressive behavior of weaned pigs after mixing.https://www.mdpi.com/1422-0067/25/1/27pigaggressive behaviorJARID2SNPmiR-9828-3p |
| spellingShingle | Huan Yang Chunlei Zhang Xiaohuan Chao Jing Zhao Mingzheng Liu Jiahao Chen Shuhan Liu Tianshuo Wang Asim Muhammad Allan P. Schinckel Bo Zhou A Functional Single Nucleotide Polymorphism in the 3′ Untranslated Region of the Porcine JARID2 Gene Is Associated with Aggressive Behavior of Weaned Pigs after Mixing International Journal of Molecular Sciences pig aggressive behavior JARID2 SNP miR-9828-3p |
| title | A Functional Single Nucleotide Polymorphism in the 3′ Untranslated Region of the Porcine JARID2 Gene Is Associated with Aggressive Behavior of Weaned Pigs after Mixing |
| title_full | A Functional Single Nucleotide Polymorphism in the 3′ Untranslated Region of the Porcine JARID2 Gene Is Associated with Aggressive Behavior of Weaned Pigs after Mixing |
| title_fullStr | A Functional Single Nucleotide Polymorphism in the 3′ Untranslated Region of the Porcine JARID2 Gene Is Associated with Aggressive Behavior of Weaned Pigs after Mixing |
| title_full_unstemmed | A Functional Single Nucleotide Polymorphism in the 3′ Untranslated Region of the Porcine JARID2 Gene Is Associated with Aggressive Behavior of Weaned Pigs after Mixing |
| title_short | A Functional Single Nucleotide Polymorphism in the 3′ Untranslated Region of the Porcine JARID2 Gene Is Associated with Aggressive Behavior of Weaned Pigs after Mixing |
| title_sort | functional single nucleotide polymorphism in the 3 untranslated region of the porcine jarid2 gene is associated with aggressive behavior of weaned pigs after mixing |
| topic | pig aggressive behavior JARID2 SNP miR-9828-3p |
| url | https://www.mdpi.com/1422-0067/25/1/27 |
| work_keys_str_mv | AT huanyang afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT chunleizhang afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT xiaohuanchao afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT jingzhao afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT mingzhengliu afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT jiahaochen afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT shuhanliu afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT tianshuowang afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT asimmuhammad afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT allanpschinckel afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT bozhou afunctionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT huanyang functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT chunleizhang functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT xiaohuanchao functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT jingzhao functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT mingzhengliu functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT jiahaochen functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT shuhanliu functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT tianshuowang functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT asimmuhammad functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT allanpschinckel functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing AT bozhou functionalsinglenucleotidepolymorphisminthe3untranslatedregionoftheporcinejarid2geneisassociatedwithaggressivebehaviorofweanedpigsaftermixing |