Identifying thrips (Insecta: Thysanoptera) using DNA Barcodes
Thrips tabaci Lindeman is an extensively distributed pest insect in many areas that affects plants through direct feeding and at the same time, it makes damage as a vector of different viruses. As a basic first step to control pests is authentic identification, but the inability to determine morphol...
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| Format: | Article |
| Language: | English |
| Published: |
Ferdowsi University of Mashhad
2010-01-01
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| Series: | Journal of Cell and Molecular Research |
| Subjects: | |
| Online Access: | https://jcmr.um.ac.ir/article_25933_77a423c9889558d2691284c83b351f6e.pdf |
| _version_ | 1798014797359349760 |
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| author | Javad Karimi Mahnaz Hassani-Kakhki Mehdi Modarres Awal |
| author_facet | Javad Karimi Mahnaz Hassani-Kakhki Mehdi Modarres Awal |
| author_sort | Javad Karimi |
| collection | DOAJ |
| description | Thrips tabaci Lindeman is an extensively distributed pest insect in many areas that affects plants through
direct feeding and at the same time, it makes damage as a vector of different viruses. As a basic first step to
control pests is authentic identification, but the inability to determine morphological characters of thrips
species makes this process very difficult. For creating an identification key for T. tabaci, an economically
important species present in Iran, four individuals were selected from four different sites of Mashhad and the
vicinity, each as a separate population. The method was based on nucleotide sequencing analysis of the
mitochondrial cytochrome C oxidase I (COI) gene. Phylogenetic analyses conducted by the neighbor-joining
method yielded almost identical phylogenetic reconstructions of trees that separated thrips based on the
geographic origin. Molecular data indicate that different thrips species are located in distinct groups. These
results show that molecular keys can be a useful method to provide much-needed information on thrips
identification for pest management officers and quarantine purposes. |
| first_indexed | 2024-04-11T15:24:09Z |
| format | Article |
| id | doaj.art-f147e5885a6b485cbd3ede4a782ae48b |
| institution | Directory Open Access Journal |
| issn | 2008-9147 2717-3364 |
| language | English |
| last_indexed | 2024-04-11T15:24:09Z |
| publishDate | 2010-01-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | Journal of Cell and Molecular Research |
| spelling | doaj.art-f147e5885a6b485cbd3ede4a782ae48b2022-12-22T04:16:18ZengFerdowsi University of MashhadJournal of Cell and Molecular Research2008-91472717-33642010-01-0121354110.22067/jcmr.v2i1.745125933Identifying thrips (Insecta: Thysanoptera) using DNA BarcodesJavad KarimiMahnaz Hassani-KakhkiMehdi Modarres AwalThrips tabaci Lindeman is an extensively distributed pest insect in many areas that affects plants through direct feeding and at the same time, it makes damage as a vector of different viruses. As a basic first step to control pests is authentic identification, but the inability to determine morphological characters of thrips species makes this process very difficult. For creating an identification key for T. tabaci, an economically important species present in Iran, four individuals were selected from four different sites of Mashhad and the vicinity, each as a separate population. The method was based on nucleotide sequencing analysis of the mitochondrial cytochrome C oxidase I (COI) gene. Phylogenetic analyses conducted by the neighbor-joining method yielded almost identical phylogenetic reconstructions of trees that separated thrips based on the geographic origin. Molecular data indicate that different thrips species are located in distinct groups. These results show that molecular keys can be a useful method to provide much-needed information on thrips identification for pest management officers and quarantine purposes.https://jcmr.um.ac.ir/article_25933_77a423c9889558d2691284c83b351f6e.pdfthrips tabacimitochondrial dnabarcodingphylogenetic treemolecular identification |
| spellingShingle | Javad Karimi Mahnaz Hassani-Kakhki Mehdi Modarres Awal Identifying thrips (Insecta: Thysanoptera) using DNA Barcodes Journal of Cell and Molecular Research thrips tabaci mitochondrial dna barcoding phylogenetic tree molecular identification |
| title | Identifying thrips (Insecta: Thysanoptera) using DNA Barcodes |
| title_full | Identifying thrips (Insecta: Thysanoptera) using DNA Barcodes |
| title_fullStr | Identifying thrips (Insecta: Thysanoptera) using DNA Barcodes |
| title_full_unstemmed | Identifying thrips (Insecta: Thysanoptera) using DNA Barcodes |
| title_short | Identifying thrips (Insecta: Thysanoptera) using DNA Barcodes |
| title_sort | identifying thrips insecta thysanoptera using dna barcodes |
| topic | thrips tabaci mitochondrial dna barcoding phylogenetic tree molecular identification |
| url | https://jcmr.um.ac.ir/article_25933_77a423c9889558d2691284c83b351f6e.pdf |
| work_keys_str_mv | AT javadkarimi identifyingthripsinsectathysanopterausingdnabarcodes AT mahnazhassanikakhki identifyingthripsinsectathysanopterausingdnabarcodes AT mehdimodarresawal identifyingthripsinsectathysanopterausingdnabarcodes |