Identification and Antimicrobial Resistance of <i>Dermatophilus congolensis</i> from Cattle in Saint Kitts and Nevis
Dermatophilosis is a form of dermatitis caused by the bacterium <i>Dermatophilus congolensis</i>. The disease usually presents as localized purulent dermatitis, crusty hair masses or widespread matting of the hair. This condition is most common in domestic ruminants; but it can also affe...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/8/7/135 |
| _version_ | 1797525951576276992 |
|---|---|
| author | Ian Branford Filip Boyen Shevaun Johnson Samantha Zayas Aspinas Chapwanya Patrick Butaye Felix N. Toka |
| author_facet | Ian Branford Filip Boyen Shevaun Johnson Samantha Zayas Aspinas Chapwanya Patrick Butaye Felix N. Toka |
| author_sort | Ian Branford |
| collection | DOAJ |
| description | Dermatophilosis is a form of dermatitis caused by the bacterium <i>Dermatophilus congolensis</i>. The disease usually presents as localized purulent dermatitis, crusty hair masses or widespread matting of the hair. This condition is most common in domestic ruminants; but it can also affect other wild animals and humans. Antimicrobial therapy is used in many regions to treat clinical dermatophilosis with varying results. In this study, we aimed to assess the antimicrobial susceptibility of <i>D. congolensis</i> isolates. Fifty-two isolates were obtained from animals showing clinical signs of the disease at farms in St. Kitts. The isolates were then confirmed as <i>D. congolensis</i> by phenotypic tests, PCR and MALDI-TOF Mass Spectrometry. Furthermore, minimum inhibitory concentrations (MIC) of 16 antimicrobial agents were determined, using the broth microdilution method. Although most antimicrobials showed MICs in line with published values, the tetracycline results displayed a clear bimodal distribution over the tested range, with most isolates showing low MICs and 6 isolates much higher values (+/− 100-fold increase). These results indicate the presence of acquired tetracycline resistance in <i>D. congolensis</i> on the island of St. Kitts. Whether the current observation has implications for efficacy of treating the disease must be confirmed in further research. |
| first_indexed | 2024-03-10T09:21:15Z |
| format | Article |
| id | doaj.art-f1487eb9265043298845d8232805ce81 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T09:21:15Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-f1487eb9265043298845d8232805ce812023-11-22T05:13:30ZengMDPI AGVeterinary Sciences2306-73812021-07-018713510.3390/vetsci8070135Identification and Antimicrobial Resistance of <i>Dermatophilus congolensis</i> from Cattle in Saint Kitts and NevisIan Branford0Filip Boyen1Shevaun Johnson2Samantha Zayas3Aspinas Chapwanya4Patrick Butaye5Felix N. Toka6Department of Biosciences, Ross University School of Veterinary Medicine, Basseterre 00334, Saint Kitts and NevisDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Biosciences, Ross University School of Veterinary Medicine, Basseterre 00334, Saint Kitts and NevisDepartment of Biosciences, Ross University School of Veterinary Medicine, Basseterre 00334, Saint Kitts and NevisDepartment of Clinical Sciences, Ross University School of Veterinary Medicine, Basseterre 00334, Saint Kitts and NevisDepartment of Biosciences, Ross University School of Veterinary Medicine, Basseterre 00334, Saint Kitts and NevisDepartment of Biosciences, Ross University School of Veterinary Medicine, Basseterre 00334, Saint Kitts and NevisDermatophilosis is a form of dermatitis caused by the bacterium <i>Dermatophilus congolensis</i>. The disease usually presents as localized purulent dermatitis, crusty hair masses or widespread matting of the hair. This condition is most common in domestic ruminants; but it can also affect other wild animals and humans. Antimicrobial therapy is used in many regions to treat clinical dermatophilosis with varying results. In this study, we aimed to assess the antimicrobial susceptibility of <i>D. congolensis</i> isolates. Fifty-two isolates were obtained from animals showing clinical signs of the disease at farms in St. Kitts. The isolates were then confirmed as <i>D. congolensis</i> by phenotypic tests, PCR and MALDI-TOF Mass Spectrometry. Furthermore, minimum inhibitory concentrations (MIC) of 16 antimicrobial agents were determined, using the broth microdilution method. Although most antimicrobials showed MICs in line with published values, the tetracycline results displayed a clear bimodal distribution over the tested range, with most isolates showing low MICs and 6 isolates much higher values (+/− 100-fold increase). These results indicate the presence of acquired tetracycline resistance in <i>D. congolensis</i> on the island of St. Kitts. Whether the current observation has implications for efficacy of treating the disease must be confirmed in further research.https://www.mdpi.com/2306-7381/8/7/135<i>Dermatophilus congolensis</i>dermatophilosisminimalinhibitoryconcentrationsusceptibility |
| spellingShingle | Ian Branford Filip Boyen Shevaun Johnson Samantha Zayas Aspinas Chapwanya Patrick Butaye Felix N. Toka Identification and Antimicrobial Resistance of <i>Dermatophilus congolensis</i> from Cattle in Saint Kitts and Nevis Veterinary Sciences <i>Dermatophilus congolensis</i> dermatophilosis minimal inhibitory concentration susceptibility |
| title | Identification and Antimicrobial Resistance of <i>Dermatophilus congolensis</i> from Cattle in Saint Kitts and Nevis |
| title_full | Identification and Antimicrobial Resistance of <i>Dermatophilus congolensis</i> from Cattle in Saint Kitts and Nevis |
| title_fullStr | Identification and Antimicrobial Resistance of <i>Dermatophilus congolensis</i> from Cattle in Saint Kitts and Nevis |
| title_full_unstemmed | Identification and Antimicrobial Resistance of <i>Dermatophilus congolensis</i> from Cattle in Saint Kitts and Nevis |
| title_short | Identification and Antimicrobial Resistance of <i>Dermatophilus congolensis</i> from Cattle in Saint Kitts and Nevis |
| title_sort | identification and antimicrobial resistance of i dermatophilus congolensis i from cattle in saint kitts and nevis |
| topic | <i>Dermatophilus congolensis</i> dermatophilosis minimal inhibitory concentration susceptibility |
| url | https://www.mdpi.com/2306-7381/8/7/135 |
| work_keys_str_mv | AT ianbranford identificationandantimicrobialresistanceofidermatophiluscongolensisifromcattleinsaintkittsandnevis AT filipboyen identificationandantimicrobialresistanceofidermatophiluscongolensisifromcattleinsaintkittsandnevis AT shevaunjohnson identificationandantimicrobialresistanceofidermatophiluscongolensisifromcattleinsaintkittsandnevis AT samanthazayas identificationandantimicrobialresistanceofidermatophiluscongolensisifromcattleinsaintkittsandnevis AT aspinaschapwanya identificationandantimicrobialresistanceofidermatophiluscongolensisifromcattleinsaintkittsandnevis AT patrickbutaye identificationandantimicrobialresistanceofidermatophiluscongolensisifromcattleinsaintkittsandnevis AT felixntoka identificationandantimicrobialresistanceofidermatophiluscongolensisifromcattleinsaintkittsandnevis |