Production of Aflatoxin B1 by <i>Aspergillus parasiticus</i> Grown on a Novel Meat-Based Media
The aim of the present study was to develop meat-based media with compositions similar to those of dry-fermented meat products and to evaluate their use in studying the growth of <i>Aspergillus parasiticus</i> and the kinetics of aflatoxin B1 (AFB1) production. In our previous experiment...
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MDPI AG
2022-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/1/25 |
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| author | Iva Zahija Barbka Jeršek Lea Demšar Mateja Lušnic Polak Tomaž Polak |
| author_facet | Iva Zahija Barbka Jeršek Lea Demšar Mateja Lušnic Polak Tomaž Polak |
| author_sort | Iva Zahija |
| collection | DOAJ |
| description | The aim of the present study was to develop meat-based media with compositions similar to those of dry-fermented meat products and to evaluate their use in studying the growth of <i>Aspergillus parasiticus</i> and the kinetics of aflatoxin B1 (AFB1) production. In our previous experiments, we found that the strain <i>A. parasiticus</i> ŽMJ7 produced a high amount of AFB1. Cooked meat agar (CMA2) was used as a novel complex meat-based medium with four variations: CMA2G (CMA2 supplemented with 1% glucose), CMA2YE (CMA2 supplemented with 0.2% yeast extract), and CMA2GYE (CMA2 supplemented with 1% glucose and 0.2% yeast extract). Media were inoculated with an <i>A. parasiticus</i> spore suspension (10<sup>5</sup> spores/mL) and incubated at 25 °C for up to 15 days. The <i>A. parasiticus</i> lag phase lasted less than 1 day, irrespective of the growth medium, with the exception of control medium CMA1 (cooked meat agar) as an already known meat-based medium. The highest mean colony growth rate was observed on CMA2 and CMA2G. Reversed-phase UPLC–MS/MS analysis was performed to determine the AFB1 concentration in combination with solid phase extraction (SPE). The highest AFB1 concentration in meat-based media was detected in CMA2GYE after 15 days of incubation (13,502 ± 2367 ng/mL media). The results showed that for studying AFB1 production in dry-fermented meat products, novel suitable media such as CMA2-based media are required. This finding could represent a potential concern with regard to the production of dry-fermented meat products. |
| first_indexed | 2024-03-09T11:06:38Z |
| format | Article |
| id | doaj.art-f1497460f6954dfbaff0f4190b9d498c |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T11:06:38Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-f1497460f6954dfbaff0f4190b9d498c2023-12-01T00:56:40ZengMDPI AGToxins2072-66512022-12-011512510.3390/toxins15010025Production of Aflatoxin B1 by <i>Aspergillus parasiticus</i> Grown on a Novel Meat-Based MediaIva Zahija0Barbka Jeršek1Lea Demšar2Mateja Lušnic Polak3Tomaž Polak4Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaThe aim of the present study was to develop meat-based media with compositions similar to those of dry-fermented meat products and to evaluate their use in studying the growth of <i>Aspergillus parasiticus</i> and the kinetics of aflatoxin B1 (AFB1) production. In our previous experiments, we found that the strain <i>A. parasiticus</i> ŽMJ7 produced a high amount of AFB1. Cooked meat agar (CMA2) was used as a novel complex meat-based medium with four variations: CMA2G (CMA2 supplemented with 1% glucose), CMA2YE (CMA2 supplemented with 0.2% yeast extract), and CMA2GYE (CMA2 supplemented with 1% glucose and 0.2% yeast extract). Media were inoculated with an <i>A. parasiticus</i> spore suspension (10<sup>5</sup> spores/mL) and incubated at 25 °C for up to 15 days. The <i>A. parasiticus</i> lag phase lasted less than 1 day, irrespective of the growth medium, with the exception of control medium CMA1 (cooked meat agar) as an already known meat-based medium. The highest mean colony growth rate was observed on CMA2 and CMA2G. Reversed-phase UPLC–MS/MS analysis was performed to determine the AFB1 concentration in combination with solid phase extraction (SPE). The highest AFB1 concentration in meat-based media was detected in CMA2GYE after 15 days of incubation (13,502 ± 2367 ng/mL media). The results showed that for studying AFB1 production in dry-fermented meat products, novel suitable media such as CMA2-based media are required. This finding could represent a potential concern with regard to the production of dry-fermented meat products.https://www.mdpi.com/2072-6651/15/1/25moulds<i>Aspergillus parasiticus</i>mycotoxinsaflatoxin B1meat-based media |
| spellingShingle | Iva Zahija Barbka Jeršek Lea Demšar Mateja Lušnic Polak Tomaž Polak Production of Aflatoxin B1 by <i>Aspergillus parasiticus</i> Grown on a Novel Meat-Based Media Toxins moulds <i>Aspergillus parasiticus</i> mycotoxins aflatoxin B1 meat-based media |
| title | Production of Aflatoxin B1 by <i>Aspergillus parasiticus</i> Grown on a Novel Meat-Based Media |
| title_full | Production of Aflatoxin B1 by <i>Aspergillus parasiticus</i> Grown on a Novel Meat-Based Media |
| title_fullStr | Production of Aflatoxin B1 by <i>Aspergillus parasiticus</i> Grown on a Novel Meat-Based Media |
| title_full_unstemmed | Production of Aflatoxin B1 by <i>Aspergillus parasiticus</i> Grown on a Novel Meat-Based Media |
| title_short | Production of Aflatoxin B1 by <i>Aspergillus parasiticus</i> Grown on a Novel Meat-Based Media |
| title_sort | production of aflatoxin b1 by i aspergillus parasiticus i grown on a novel meat based media |
| topic | moulds <i>Aspergillus parasiticus</i> mycotoxins aflatoxin B1 meat-based media |
| url | https://www.mdpi.com/2072-6651/15/1/25 |
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