Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling
Exhaled ammonia (NH3) is an essential noninvasive biomarker for disease diagnosis. In this study, an acetone-modifier positive photoionization ion mobility spectrometry (AM-PIMS) method was developed for accurate qualitative and quantitative analysis of exhaled NH3 with high selectivity and sensitiv...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Journal of Pharmaceutical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177923000230 |
| _version_ | 1797837810833555456 |
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| author | Lu Wang Dandan Jiang Lei Hua Chuang Chen Dongming Li Weiguo Wang Yiqian Xu Qimu Yang Haiyang Li Song Leng |
| author_facet | Lu Wang Dandan Jiang Lei Hua Chuang Chen Dongming Li Weiguo Wang Yiqian Xu Qimu Yang Haiyang Li Song Leng |
| author_sort | Lu Wang |
| collection | DOAJ |
| description | Exhaled ammonia (NH3) is an essential noninvasive biomarker for disease diagnosis. In this study, an acetone-modifier positive photoionization ion mobility spectrometry (AM-PIMS) method was developed for accurate qualitative and quantitative analysis of exhaled NH3 with high selectivity and sensitivity. Acetone was introduced into the drift tube along with the drift gas as a modifier, and the characteristic NH3 product ion peak of (C3H6O)4NH4+ (K0 = 1.45 cm2/V·s) was obtained through the ion-molecule reaction with acetone reactant ions (C3H6O)2H+ (K0 = 1.87 cm2/V·s), which significantly increased the peak-to-peak resolution and improved the accuracy of exhaled NH3 qualitative identification. Moreover, the interference of high humidity and the memory effect of NH3 molecules were significantly reduced via online dilution and purging sampling, thus realizing breath-by-breath measurement. As a result, a wide quantitative range of 5.87–140.92 μmol/L with a response time of 40 ms was achieved, and the exhaled NH3 profile could be synchronized with the concentration curve of exhaled CO2. Finally, the analytical capacity of AM-PIMS was demonstrated by measuring the exhaled NH3 of healthy subjects, demonstrating its great potential for clinical disease diagnosis. |
| first_indexed | 2024-04-09T15:30:40Z |
| format | Article |
| id | doaj.art-f14a8cbd88f34fcd8d6b5822cccacce9 |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-04-09T15:30:40Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
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| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-f14a8cbd88f34fcd8d6b5822cccacce92023-04-28T08:55:21ZengElsevierJournal of Pharmaceutical Analysis2095-17792023-04-01134412420Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging samplingLu Wang0Dandan Jiang1Lei Hua2Chuang Chen3Dongming Li4Weiguo Wang5Yiqian Xu6Qimu Yang7Haiyang Li8Song Leng9CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Health Management Center, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Dalian Key Laboratory for Online Analytical Instrumentation, Dalian, 116023, China; Dalian Engineering Research Center of Breath Diagnostic Technology, Dalian, 116023, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Dalian Key Laboratory for Online Analytical Instrumentation, Dalian, 116023, China; Dalian Engineering Research Center of Breath Diagnostic Technology, Dalian, 116023, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Dalian Key Laboratory for Online Analytical Instrumentation, Dalian, 116023, China; Dalian Engineering Research Center of Breath Diagnostic Technology, Dalian, 116023, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Dalian Key Laboratory for Online Analytical Instrumentation, Dalian, 116023, China; Dalian Engineering Research Center of Breath Diagnostic Technology, Dalian, 116023, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Dalian Key Laboratory for Online Analytical Instrumentation, Dalian, 116023, China; Dalian Engineering Research Center of Breath Diagnostic Technology, Dalian, 116023, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Dalian Key Laboratory for Online Analytical Instrumentation, Dalian, 116023, China; Dalian Engineering Research Center of Breath Diagnostic Technology, Dalian, 116023, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Dalian Key Laboratory for Online Analytical Instrumentation, Dalian, 116023, China; Dalian Engineering Research Center of Breath Diagnostic Technology, Dalian, 116023, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Dalian Key Laboratory for Online Analytical Instrumentation, Dalian, 116023, China; Dalian Engineering Research Center of Breath Diagnostic Technology, Dalian, 116023, China; Corresponding author. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.Health Management Center, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, China; Corresponding author. Health Management Center, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, China.Exhaled ammonia (NH3) is an essential noninvasive biomarker for disease diagnosis. In this study, an acetone-modifier positive photoionization ion mobility spectrometry (AM-PIMS) method was developed for accurate qualitative and quantitative analysis of exhaled NH3 with high selectivity and sensitivity. Acetone was introduced into the drift tube along with the drift gas as a modifier, and the characteristic NH3 product ion peak of (C3H6O)4NH4+ (K0 = 1.45 cm2/V·s) was obtained through the ion-molecule reaction with acetone reactant ions (C3H6O)2H+ (K0 = 1.87 cm2/V·s), which significantly increased the peak-to-peak resolution and improved the accuracy of exhaled NH3 qualitative identification. Moreover, the interference of high humidity and the memory effect of NH3 molecules were significantly reduced via online dilution and purging sampling, thus realizing breath-by-breath measurement. As a result, a wide quantitative range of 5.87–140.92 μmol/L with a response time of 40 ms was achieved, and the exhaled NH3 profile could be synchronized with the concentration curve of exhaled CO2. Finally, the analytical capacity of AM-PIMS was demonstrated by measuring the exhaled NH3 of healthy subjects, demonstrating its great potential for clinical disease diagnosis.http://www.sciencedirect.com/science/article/pii/S2095177923000230Photoionization ion mobility spectrometryAcetone modifierExhaled ammoniaBreath analysis |
| spellingShingle | Lu Wang Dandan Jiang Lei Hua Chuang Chen Dongming Li Weiguo Wang Yiqian Xu Qimu Yang Haiyang Li Song Leng Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling Journal of Pharmaceutical Analysis Photoionization ion mobility spectrometry Acetone modifier Exhaled ammonia Breath analysis |
| title | Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling |
| title_full | Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling |
| title_fullStr | Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling |
| title_full_unstemmed | Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling |
| title_short | Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling |
| title_sort | breath by breath measurement of exhaled ammonia by acetone modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling |
| topic | Photoionization ion mobility spectrometry Acetone modifier Exhaled ammonia Breath analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2095177923000230 |
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