Comparison of Tissue Factors in the Ontogenetic Aspects of Human Cholesteatoma
Background: An acquired cholesteatoma is a benign but locally aggressive lesion in the middle ear. It is characterized by chronic inflammation and the destruction of surrounding bone. Therefore, the aim of this study was to compare defensins HβD-2 and HβD-4; pro- and anti-inflammatory cytokines IL-1...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/14/6/662 |
| _version_ | 1797241485351976960 |
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| author | Kristaps Dambergs Gunta Sumeraga Māra Pilmane |
| author_facet | Kristaps Dambergs Gunta Sumeraga Māra Pilmane |
| author_sort | Kristaps Dambergs |
| collection | DOAJ |
| description | Background: An acquired cholesteatoma is a benign but locally aggressive lesion in the middle ear. It is characterized by chronic inflammation and the destruction of surrounding bone. Therefore, the aim of this study was to compare defensins HβD-2 and HβD-4; pro- and anti-inflammatory cytokines IL-1α and IL-10; proliferation marker Ki-67; transcription factor NF-κβ; angiogenetic factor VEGF; Sonic hedgehog gene protein SHH; and remodeling factors MMP-2, MMP-9, TIMP-2, and TIMP-4 in adult and pediatric cholesteatoma tissue, and to compare these groups with control skin tissue. Methods: The study included 25 cholesteatoma tissue material samples from children, 25 from adults, and 7 deep external ear canal skin samples from cadavers. The tissues were stained immunohistochemically and evaluated using semi-quantitative methods. Nonparametric tests, such as the Kruskal–Wallis test and Spearman rank correlation, were used. Results: There were no statistically discernible differences between the adult and children groups when comparing the relative numbers of factor-positive cells. Conclusions: There are no histopathological differences between adult and children cholesteatoma tissues. |
| first_indexed | 2024-04-24T18:24:04Z |
| format | Article |
| id | doaj.art-f14b75a39a544f26b08a07d690888cf3 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-04-24T18:24:04Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-f14b75a39a544f26b08a07d690888cf32024-03-27T13:33:28ZengMDPI AGDiagnostics2075-44182024-03-0114666210.3390/diagnostics14060662Comparison of Tissue Factors in the Ontogenetic Aspects of Human CholesteatomaKristaps Dambergs0Gunta Sumeraga1Māra Pilmane2Department of Otorhinolaryngology, Riga Stradiņš University, Pilsonu Street 13, LV-1002 Riga, LatviaDepartment of Otorhinolaryngology, Riga Stradiņš University, Pilsonu Street 13, LV-1002 Riga, LatviaDepartment of Morphology, Institute of Anatomy and Anthropology, Riga Stradiņš University, LV-1007 Riga, LatviaBackground: An acquired cholesteatoma is a benign but locally aggressive lesion in the middle ear. It is characterized by chronic inflammation and the destruction of surrounding bone. Therefore, the aim of this study was to compare defensins HβD-2 and HβD-4; pro- and anti-inflammatory cytokines IL-1α and IL-10; proliferation marker Ki-67; transcription factor NF-κβ; angiogenetic factor VEGF; Sonic hedgehog gene protein SHH; and remodeling factors MMP-2, MMP-9, TIMP-2, and TIMP-4 in adult and pediatric cholesteatoma tissue, and to compare these groups with control skin tissue. Methods: The study included 25 cholesteatoma tissue material samples from children, 25 from adults, and 7 deep external ear canal skin samples from cadavers. The tissues were stained immunohistochemically and evaluated using semi-quantitative methods. Nonparametric tests, such as the Kruskal–Wallis test and Spearman rank correlation, were used. Results: There were no statistically discernible differences between the adult and children groups when comparing the relative numbers of factor-positive cells. Conclusions: There are no histopathological differences between adult and children cholesteatoma tissues.https://www.mdpi.com/2075-4418/14/6/662cholesteatomadefensinscytokinesKi-67transcription factorsvascular endothelial growth factor |
| spellingShingle | Kristaps Dambergs Gunta Sumeraga Māra Pilmane Comparison of Tissue Factors in the Ontogenetic Aspects of Human Cholesteatoma Diagnostics cholesteatoma defensins cytokines Ki-67 transcription factors vascular endothelial growth factor |
| title | Comparison of Tissue Factors in the Ontogenetic Aspects of Human Cholesteatoma |
| title_full | Comparison of Tissue Factors in the Ontogenetic Aspects of Human Cholesteatoma |
| title_fullStr | Comparison of Tissue Factors in the Ontogenetic Aspects of Human Cholesteatoma |
| title_full_unstemmed | Comparison of Tissue Factors in the Ontogenetic Aspects of Human Cholesteatoma |
| title_short | Comparison of Tissue Factors in the Ontogenetic Aspects of Human Cholesteatoma |
| title_sort | comparison of tissue factors in the ontogenetic aspects of human cholesteatoma |
| topic | cholesteatoma defensins cytokines Ki-67 transcription factors vascular endothelial growth factor |
| url | https://www.mdpi.com/2075-4418/14/6/662 |
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