Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid Haemorrhage
Aneurysmal subarachnoid haemorrhages (aSAH) account for 5% of strokes and continues to place a great burden on patients and their families. Cerebral vasospasm (CVS) is one of the main causes of death after aSAH, and is usually diagnosed between day 3 and 14 after bleeding. Its pathogenesis remains p...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Journal of Clinical Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0383/11/2/380 |
| _version_ | 1797492999687503872 |
|---|---|
| author | Ernest Jan Bobeff Malgorzata Bukowiecka-Matusiak Konrad Stawiski Karol Wiśniewski Izabela Burzynska-Pedziwiatr Magdalena Kordzińska Konrad Kowalski Przemyslaw Sendys Michał Piotrowski Dorota Szczesna Ludomir Stefańczyk Lucyna Alicja Wozniak Dariusz Jan Jaskólski |
| author_facet | Ernest Jan Bobeff Malgorzata Bukowiecka-Matusiak Konrad Stawiski Karol Wiśniewski Izabela Burzynska-Pedziwiatr Magdalena Kordzińska Konrad Kowalski Przemyslaw Sendys Michał Piotrowski Dorota Szczesna Ludomir Stefańczyk Lucyna Alicja Wozniak Dariusz Jan Jaskólski |
| author_sort | Ernest Jan Bobeff |
| collection | DOAJ |
| description | Aneurysmal subarachnoid haemorrhages (aSAH) account for 5% of strokes and continues to place a great burden on patients and their families. Cerebral vasospasm (CVS) is one of the main causes of death after aSAH, and is usually diagnosed between day 3 and 14 after bleeding. Its pathogenesis remains poorly understood. To verify whether plasma concentration of amino acids have prognostic value in predicting CVS, we analysed data from 35 patients after aSAH (median age 55 years, IQR 39–62; 20 females, 57.1%), and 37 healthy volunteers (median age 50 years, IQR 38–56; 19 females, 51.4%). Fasting peripheral blood samples were collected on postoperative day one and seven. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis was performed. The results showed that plasma from patients after aSAH featured a distinctive amino acids concentration which was presented in both principal component analysis and direct comparison. No significant differences were noted between postoperative day one and seven. A total of 18 patients from the study group (51.4%) developed CVS. Hydroxyproline (AUC = 0.7042, 95%CI 0.5259–0.8826, <i>p</i> = 0.0248) and phenylalanine (AUC = 0.6944, 95%CI 0.5119–0.877, <i>p</i> = 0.0368) presented significant CVS prediction potential. Combining the Hunt-Hess Scale and plasma levels of hydroxyproline and phenylalanine provided the model with the best predictive performance and the lowest leave-one-out cross-validation of performance error. Our results suggest that plasma amino acids may improve sensitivity and specificity of Hunt-Hess scale in predicting CVS. |
| first_indexed | 2024-03-10T01:14:41Z |
| format | Article |
| id | doaj.art-f14df1537e2843d09a417bd380abfded |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T01:14:41Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-f14df1537e2843d09a417bd380abfded2023-11-23T14:13:00ZengMDPI AGJournal of Clinical Medicine2077-03832022-01-0111238010.3390/jcm11020380Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid HaemorrhageErnest Jan Bobeff0Malgorzata Bukowiecka-Matusiak1Konrad Stawiski2Karol Wiśniewski3Izabela Burzynska-Pedziwiatr4Magdalena Kordzińska5Konrad Kowalski6Przemyslaw Sendys7Michał Piotrowski8Dorota Szczesna9Ludomir Stefańczyk10Lucyna Alicja Wozniak11Dariusz Jan Jaskólski12Department of Neurosurgery and Neuro-Oncology, Medical University of Lodz, Barlicki University Hospital, Kopcinskiego St. 22, 90-153 Lodz, PolandDepartment of Structural Biology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Biostatistics and Translational Medicine, Medical University of Lodz, Mazowiecka 15 Street, 92-215 Lodz, PolandDepartment of Neurosurgery and Neuro-Oncology, Medical University of Lodz, Barlicki University Hospital, Kopcinskiego St. 22, 90-153 Lodz, PolandDepartment of Structural Biology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Radiology, Barlicki Memorial Teaching Hospital, Medical University of Lodz, Kopcinskiego 22 Street, 90-153 Lodz, PolandLaboratorium Diagnostyczne Masdiag, ul. Żeromskiego 33, 01-882 Warszawa, PolandLaboratorium Diagnostyczne Masdiag, ul. Żeromskiego 33, 01-882 Warszawa, PolandDepartment of Neurosurgery and Neuro-Oncology, Medical University of Lodz, Barlicki University Hospital, Kopcinskiego St. 22, 90-153 Lodz, PolandDepartment of Structural Biology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Radiology, Barlicki Memorial Teaching Hospital, Medical University of Lodz, Kopcinskiego 22 Street, 90-153 Lodz, PolandDepartment of Structural Biology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Neurosurgery and Neuro-Oncology, Medical University of Lodz, Barlicki University Hospital, Kopcinskiego St. 22, 90-153 Lodz, PolandAneurysmal subarachnoid haemorrhages (aSAH) account for 5% of strokes and continues to place a great burden on patients and their families. Cerebral vasospasm (CVS) is one of the main causes of death after aSAH, and is usually diagnosed between day 3 and 14 after bleeding. Its pathogenesis remains poorly understood. To verify whether plasma concentration of amino acids have prognostic value in predicting CVS, we analysed data from 35 patients after aSAH (median age 55 years, IQR 39–62; 20 females, 57.1%), and 37 healthy volunteers (median age 50 years, IQR 38–56; 19 females, 51.4%). Fasting peripheral blood samples were collected on postoperative day one and seven. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis was performed. The results showed that plasma from patients after aSAH featured a distinctive amino acids concentration which was presented in both principal component analysis and direct comparison. No significant differences were noted between postoperative day one and seven. A total of 18 patients from the study group (51.4%) developed CVS. Hydroxyproline (AUC = 0.7042, 95%CI 0.5259–0.8826, <i>p</i> = 0.0248) and phenylalanine (AUC = 0.6944, 95%CI 0.5119–0.877, <i>p</i> = 0.0368) presented significant CVS prediction potential. Combining the Hunt-Hess Scale and plasma levels of hydroxyproline and phenylalanine provided the model with the best predictive performance and the lowest leave-one-out cross-validation of performance error. Our results suggest that plasma amino acids may improve sensitivity and specificity of Hunt-Hess scale in predicting CVS.https://www.mdpi.com/2077-0383/11/2/380aneurysmal subarachnoid haemorrhagecerebral vasospasmplasma amino acidspredictorshigh performance liquid chromatography-mass spectrometryHunt-Hess scale |
| spellingShingle | Ernest Jan Bobeff Malgorzata Bukowiecka-Matusiak Konrad Stawiski Karol Wiśniewski Izabela Burzynska-Pedziwiatr Magdalena Kordzińska Konrad Kowalski Przemyslaw Sendys Michał Piotrowski Dorota Szczesna Ludomir Stefańczyk Lucyna Alicja Wozniak Dariusz Jan Jaskólski Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid Haemorrhage Journal of Clinical Medicine aneurysmal subarachnoid haemorrhage cerebral vasospasm plasma amino acids predictors high performance liquid chromatography-mass spectrometry Hunt-Hess scale |
| title | Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid Haemorrhage |
| title_full | Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid Haemorrhage |
| title_fullStr | Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid Haemorrhage |
| title_full_unstemmed | Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid Haemorrhage |
| title_short | Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid Haemorrhage |
| title_sort | plasma amino acids may improve prediction accuracy of cerebral vasospasm after aneurysmal subarachnoid haemorrhage |
| topic | aneurysmal subarachnoid haemorrhage cerebral vasospasm plasma amino acids predictors high performance liquid chromatography-mass spectrometry Hunt-Hess scale |
| url | https://www.mdpi.com/2077-0383/11/2/380 |
| work_keys_str_mv | AT ernestjanbobeff plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT malgorzatabukowieckamatusiak plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT konradstawiski plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT karolwisniewski plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT izabelaburzynskapedziwiatr plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT magdalenakordzinska plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT konradkowalski plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT przemyslawsendys plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT michałpiotrowski plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT dorotaszczesna plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT ludomirstefanczyk plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT lucynaalicjawozniak plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage AT dariuszjanjaskolski plasmaaminoacidsmayimprovepredictionaccuracyofcerebralvasospasmafteraneurysmalsubarachnoidhaemorrhage |