Exploration the homeostasis of signaling molecules in monocotyledonous crops with different CuO nanoparticle tolerance
Copper is an essential microelement that is indispensable for plant growth and development. The use of copper oxide nanoparticles (CuO NPs) in industry and agriculture has also increased because of their beneficial properties. However, excess amounts of CuO NPs may negatively affect the growth of mo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Plant Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X23000155 |
| _version_ | 1797869726164058112 |
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| author | Barbara Kacziba Ádám Szierer Enikő Mészáros Andrea Rónavári Zoltán Kónya Gábor Feigl |
| author_facet | Barbara Kacziba Ádám Szierer Enikő Mészáros Andrea Rónavári Zoltán Kónya Gábor Feigl |
| author_sort | Barbara Kacziba |
| collection | DOAJ |
| description | Copper is an essential microelement that is indispensable for plant growth and development. The use of copper oxide nanoparticles (CuO NPs) in industry and agriculture has also increased because of their beneficial properties. However, excess amounts of CuO NPs may negatively affect the growth of monocotyledonous plant species, primarily through the generation of reactive oxygen species, which results in oxidative stress. Despite their increasingly widespread use, little is known regarding the signaling processes responsible for the effects of CuO NPs on the growth of monocotyledonous crops, or their impact on the homeostasis of reactive nitrogen species, hydrogen sulfide, and protein tyrosine nitration.In this study, the concentration of CuO NP that inhibits 50% of root growth was determined using sorghum, wheat, rye, and triticale as model plant species, and the NP-induced stress response and the balance of reactive molecules were assessed. Based on the effective concentration of CuO NP, wheat, rye, and triticale were more tolerant compared with sorghum, and entirely different response mechanisms in the homeostasis of reactive oxygen, nitrogen and sulfur species were observed. For the sensitive sorghum roots, the amount of reactive molecules was not significantly altered, whereas a significant increase in protein tyrosine nitration indicated a severely stressful state caused by CuO NPs. In contrast, the amount of reactive molecules increased significantly in the roots of the relatively tolerant species, and while the appearance of lipid peroxidation indicated oxidative stress, different changes in protein tyrosine nitration was associated with tolerance. The significant CuO NP-induced rise of endogenous H2S content in the root tips may be partly responsible for the relative tolerance of wheat, rye, and triticale compared with sorghum. CuO NP stress induced distinct modifications in the root tip cell walls of the examined species, where lignification was observed in the relatively sensitive sorghum, while in the tolerant species only callose deposition was detected. Overall, our results demonstrate that while monocotyledonous species with different CuO NP sensitivities may exhibit similar growth responses, the underlying changes in the dynamics of reactive molecules influence their tolerance. |
| first_indexed | 2024-04-10T00:16:04Z |
| format | Article |
| id | doaj.art-f14fc648c26e46bdacc5e1c8ca52fb6b |
| institution | Directory Open Access Journal |
| issn | 2667-064X |
| language | English |
| last_indexed | 2024-04-10T00:16:04Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj.art-f14fc648c26e46bdacc5e1c8ca52fb6b2023-03-16T05:06:54ZengElsevierPlant Stress2667-064X2023-03-017100145Exploration the homeostasis of signaling molecules in monocotyledonous crops with different CuO nanoparticle toleranceBarbara Kacziba0Ádám Szierer1Enikő Mészáros2Andrea Rónavári3Zoltán Kónya4Gábor Feigl5Department of Plant Biology, University of Szeged, Szeged, HungaryDepartment of Plant Biology, University of Szeged, Szeged, HungaryDepartment of Plant Biology, University of Szeged, Szeged, HungaryDepartment of Applied and Environmental Chemistry, University of Szeged, Szeged, HungaryDepartment of Applied and Environmental Chemistry, University of Szeged, Szeged, HungaryDepartment of Plant Biology, University of Szeged, Szeged, Hungary; Corresponding author: Gábor Feigl, H-6726 Szeged, Közép fasor 52, Hungary.Copper is an essential microelement that is indispensable for plant growth and development. The use of copper oxide nanoparticles (CuO NPs) in industry and agriculture has also increased because of their beneficial properties. However, excess amounts of CuO NPs may negatively affect the growth of monocotyledonous plant species, primarily through the generation of reactive oxygen species, which results in oxidative stress. Despite their increasingly widespread use, little is known regarding the signaling processes responsible for the effects of CuO NPs on the growth of monocotyledonous crops, or their impact on the homeostasis of reactive nitrogen species, hydrogen sulfide, and protein tyrosine nitration.In this study, the concentration of CuO NP that inhibits 50% of root growth was determined using sorghum, wheat, rye, and triticale as model plant species, and the NP-induced stress response and the balance of reactive molecules were assessed. Based on the effective concentration of CuO NP, wheat, rye, and triticale were more tolerant compared with sorghum, and entirely different response mechanisms in the homeostasis of reactive oxygen, nitrogen and sulfur species were observed. For the sensitive sorghum roots, the amount of reactive molecules was not significantly altered, whereas a significant increase in protein tyrosine nitration indicated a severely stressful state caused by CuO NPs. In contrast, the amount of reactive molecules increased significantly in the roots of the relatively tolerant species, and while the appearance of lipid peroxidation indicated oxidative stress, different changes in protein tyrosine nitration was associated with tolerance. The significant CuO NP-induced rise of endogenous H2S content in the root tips may be partly responsible for the relative tolerance of wheat, rye, and triticale compared with sorghum. CuO NP stress induced distinct modifications in the root tip cell walls of the examined species, where lignification was observed in the relatively sensitive sorghum, while in the tolerant species only callose deposition was detected. Overall, our results demonstrate that while monocotyledonous species with different CuO NP sensitivities may exhibit similar growth responses, the underlying changes in the dynamics of reactive molecules influence their tolerance.http://www.sciencedirect.com/science/article/pii/S2667064X23000155Copper oxide nanoparticlesMonocotyledonous cropsSorghumWheatRyeTriticale |
| spellingShingle | Barbara Kacziba Ádám Szierer Enikő Mészáros Andrea Rónavári Zoltán Kónya Gábor Feigl Exploration the homeostasis of signaling molecules in monocotyledonous crops with different CuO nanoparticle tolerance Plant Stress Copper oxide nanoparticles Monocotyledonous crops Sorghum Wheat Rye Triticale |
| title | Exploration the homeostasis of signaling molecules in monocotyledonous crops with different CuO nanoparticle tolerance |
| title_full | Exploration the homeostasis of signaling molecules in monocotyledonous crops with different CuO nanoparticle tolerance |
| title_fullStr | Exploration the homeostasis of signaling molecules in monocotyledonous crops with different CuO nanoparticle tolerance |
| title_full_unstemmed | Exploration the homeostasis of signaling molecules in monocotyledonous crops with different CuO nanoparticle tolerance |
| title_short | Exploration the homeostasis of signaling molecules in monocotyledonous crops with different CuO nanoparticle tolerance |
| title_sort | exploration the homeostasis of signaling molecules in monocotyledonous crops with different cuo nanoparticle tolerance |
| topic | Copper oxide nanoparticles Monocotyledonous crops Sorghum Wheat Rye Triticale |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X23000155 |
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