Comparison of the effect of three autogenous bone harvesting methods on cell viability in rabbits
Background. This study was designed to compare the viability of autogenous bone grafts, harvested using different methods, in order to determine the best harvesting technique with respect to more viable cells. Methods. In this animal experimental study, three harvesting methods, including manual ins...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2017-06-01
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| Series: | Journal of Dental Research, Dental Clinics, Dental Prospects |
| Subjects: | |
| Online Access: | http://joddd.tbzmed.ac.ir/PDF/joddd-11-73.pdf |
| Summary: | Background. This study was designed to
compare the viability of autogenous bone grafts, harvested using different
methods, in order to determine the best harvesting technique with respect to
more viable cells.
Methods. In this animal experimental study, three harvesting methods, including
manual instrument (chisel), rotary device and piezosurgery, were used for
harvesting bone grafts from the lateral body of the mandible on the left and
right sides of 10 rabbits. In each group, 20 bone samples were collected and
their viability was assessed using MTS kit. Statistical analyses, including
ANOVA and post hoc Tukey tests, were used for evaluating significant differences
between the groups.
Results. One-way ANOVA showed significant differences
between all the groups (P=0.000). Data analysis using post hoc Tukey tests
indicated that manual instrument and piezosurgery had no significant
differences with regard to cell viability (P=0.749) and the cell viability in
both groups was higher than that with the use of a rotary instrument
(P=0.000).
Conclusion. Autogenous
bone grafts harvested with a manual instrument and piezosurgery had more
viable cells in comparison to the bone chips harvested with a rotary device. |
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| ISSN: | 2008-210X 2008-2118 |