Penetration Process of a Hydrated Deep Eutectic Solvent Through the Stratum Corneum and its Application as a Protein Penetration Enhancer
Abstract The penetration mechanism of choline chloride‐glycerol deep eutectic solvent (DES) through the stratum corneum (SC) as a potential solvent for a novel enhancer of protein penetration into the skin was investigated in a wide and small angle X‐ray diffraction study. We found that DES penetrat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2020-09-01
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| Series: | ChemistryOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/open.202000114 |
| _version_ | 1818620783194275840 |
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| author | Dr. Mina Sakuragi Emika Maeda Prof. Katsuki Kusakabe |
| author_facet | Dr. Mina Sakuragi Emika Maeda Prof. Katsuki Kusakabe |
| author_sort | Dr. Mina Sakuragi |
| collection | DOAJ |
| description | Abstract The penetration mechanism of choline chloride‐glycerol deep eutectic solvent (DES) through the stratum corneum (SC) as a potential solvent for a novel enhancer of protein penetration into the skin was investigated in a wide and small angle X‐ray diffraction study. We found that DES penetrated through intercellular lipids but not the corneocytes. DES seemed to extract a portion of lipids of the short lamellae in the SC. Hydrated DES with a DES to water weight ratio of 9 to 1 (9DES‐1H2O) showed the strongest interaction with the lipids in the SC compared with water, DES, and hydrated DESs with another weight ratio of DES to water (DES : water=8 : 2). In a skin penetration test with a fluorescently labelled lysozyme, 9DES‐1H2O increased the amount of penetration through the SC by two‐fold compared with HEPES buffer. |
| first_indexed | 2024-12-16T17:58:52Z |
| format | Article |
| id | doaj.art-f15212ed5b544ebf9217e707a50c836e |
| institution | Directory Open Access Journal |
| issn | 2191-1363 |
| language | English |
| last_indexed | 2024-12-16T17:58:52Z |
| publishDate | 2020-09-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemistryOpen |
| spelling | doaj.art-f15212ed5b544ebf9217e707a50c836e2022-12-21T22:22:06ZengWiley-VCHChemistryOpen2191-13632020-09-019995395810.1002/open.202000114Penetration Process of a Hydrated Deep Eutectic Solvent Through the Stratum Corneum and its Application as a Protein Penetration EnhancerDr. Mina Sakuragi0Emika Maeda1Prof. Katsuki Kusakabe2Faculty of Engineering Department of Nanoscience Sojo University 4-22-1 Ikeda, Nishi-ku Kumamoto City 860-0082 JapanFaculty of Engineering Department of Nanoscience Sojo University 4-22-1 Ikeda, Nishi-ku Kumamoto City 860-0082 JapanFaculty of Engineering Department of Nanoscience Sojo University 4-22-1 Ikeda, Nishi-ku Kumamoto City 860-0082 JapanAbstract The penetration mechanism of choline chloride‐glycerol deep eutectic solvent (DES) through the stratum corneum (SC) as a potential solvent for a novel enhancer of protein penetration into the skin was investigated in a wide and small angle X‐ray diffraction study. We found that DES penetrated through intercellular lipids but not the corneocytes. DES seemed to extract a portion of lipids of the short lamellae in the SC. Hydrated DES with a DES to water weight ratio of 9 to 1 (9DES‐1H2O) showed the strongest interaction with the lipids in the SC compared with water, DES, and hydrated DESs with another weight ratio of DES to water (DES : water=8 : 2). In a skin penetration test with a fluorescently labelled lysozyme, 9DES‐1H2O increased the amount of penetration through the SC by two‐fold compared with HEPES buffer.https://doi.org/10.1002/open.202000114deep eutectic solventsdrug deliverystratum corneumlysozymeX-ray diffraction |
| spellingShingle | Dr. Mina Sakuragi Emika Maeda Prof. Katsuki Kusakabe Penetration Process of a Hydrated Deep Eutectic Solvent Through the Stratum Corneum and its Application as a Protein Penetration Enhancer ChemistryOpen deep eutectic solvents drug delivery stratum corneum lysozyme X-ray diffraction |
| title | Penetration Process of a Hydrated Deep Eutectic Solvent Through the Stratum Corneum and its Application as a Protein Penetration Enhancer |
| title_full | Penetration Process of a Hydrated Deep Eutectic Solvent Through the Stratum Corneum and its Application as a Protein Penetration Enhancer |
| title_fullStr | Penetration Process of a Hydrated Deep Eutectic Solvent Through the Stratum Corneum and its Application as a Protein Penetration Enhancer |
| title_full_unstemmed | Penetration Process of a Hydrated Deep Eutectic Solvent Through the Stratum Corneum and its Application as a Protein Penetration Enhancer |
| title_short | Penetration Process of a Hydrated Deep Eutectic Solvent Through the Stratum Corneum and its Application as a Protein Penetration Enhancer |
| title_sort | penetration process of a hydrated deep eutectic solvent through the stratum corneum and its application as a protein penetration enhancer |
| topic | deep eutectic solvents drug delivery stratum corneum lysozyme X-ray diffraction |
| url | https://doi.org/10.1002/open.202000114 |
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