Cytostatic Effects of Avocado Oil Using Single-cell Gel Electrophoresis (Comet Assay)
The goal of this paper is to assess the mutagenicand genotoxic potentials of avocado oil made from the fruit pulp of Persea Americana, a member of the Lauraceae family.Michigan Cancer Foundation-7 (MCF-7) cells are used in the 3-4,5 dimethylthiazol-2yl-2,5-diphenyl tetrazolium bromide (MTT) test to...
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| Format: | Article |
| Language: | English |
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Koya University
2023-01-01
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| Series: | ARO-The Scientific Journal of Koya University |
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| Online Access: | https://aro.koyauniversity.org/index.php/aro/article/view/1073 |
| _version_ | 1797704961591607296 |
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| author | Subasini Uthirapathy |
| author_facet | Subasini Uthirapathy |
| author_sort | Subasini Uthirapathy |
| collection | DOAJ |
| description |
The goal of this paper is to assess the mutagenicand genotoxic potentials of avocado oil made from the fruit pulp of Persea Americana, a member of the Lauraceae family.Michigan Cancer Foundation-7 (MCF-7) cells are used in the 3-4,5 dimethylthiazol-2yl-2,5-diphenyl tetrazolium bromide (MTT) test to examine the possible antiproliferative and cytostatic qualities of different doses of avocado oil, and MCF-7 cells are used in the comet assay to examine the potential cytostatic effects of avocado oil extracted from the avocado fruit. DNA in human breast cancer cells is partially damaged by avocado oil. However, DNA damage at low, medium, and high levels was discovered in the positive control. Without positive control, the DNA damage level falls in the low, middle, and high ranges. The MTT assay shows that avocado oil exerts a dose-dependent cytostatic impact on human breast cancer MCF-7 cells with an IC50 value of 379.2 μg/mL, which is the IC50 value that causes genotoxicity in the comet assay.
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| first_indexed | 2024-03-12T05:29:10Z |
| format | Article |
| id | doaj.art-f156ec293da940eab737e00e0b4c1951 |
| institution | Directory Open Access Journal |
| issn | 2410-9355 2307-549X |
| language | English |
| last_indexed | 2024-03-12T05:29:10Z |
| publishDate | 2023-01-01 |
| publisher | Koya University |
| record_format | Article |
| series | ARO-The Scientific Journal of Koya University |
| spelling | doaj.art-f156ec293da940eab737e00e0b4c19512023-09-03T07:07:40ZengKoya UniversityARO-The Scientific Journal of Koya University2410-93552307-549X2023-01-0111110.14500/aro.11073Cytostatic Effects of Avocado Oil Using Single-cell Gel Electrophoresis (Comet Assay)Subasini Uthirapathy0Department of Pharmacology, Faculty of Pharmacy, Tishk International University, Erbil, Kurdistan Region, Iraq The goal of this paper is to assess the mutagenicand genotoxic potentials of avocado oil made from the fruit pulp of Persea Americana, a member of the Lauraceae family.Michigan Cancer Foundation-7 (MCF-7) cells are used in the 3-4,5 dimethylthiazol-2yl-2,5-diphenyl tetrazolium bromide (MTT) test to examine the possible antiproliferative and cytostatic qualities of different doses of avocado oil, and MCF-7 cells are used in the comet assay to examine the potential cytostatic effects of avocado oil extracted from the avocado fruit. DNA in human breast cancer cells is partially damaged by avocado oil. However, DNA damage at low, medium, and high levels was discovered in the positive control. Without positive control, the DNA damage level falls in the low, middle, and high ranges. The MTT assay shows that avocado oil exerts a dose-dependent cytostatic impact on human breast cancer MCF-7 cells with an IC50 value of 379.2 μg/mL, which is the IC50 value that causes genotoxicity in the comet assay. https://aro.koyauniversity.org/index.php/aro/article/view/1073Avocado oilPersea AmericanaGenotoxicityMutagenicCytostatic |
| spellingShingle | Subasini Uthirapathy Cytostatic Effects of Avocado Oil Using Single-cell Gel Electrophoresis (Comet Assay) ARO-The Scientific Journal of Koya University Avocado oil Persea Americana Genotoxicity Mutagenic Cytostatic |
| title | Cytostatic Effects of Avocado Oil Using Single-cell Gel Electrophoresis (Comet Assay) |
| title_full | Cytostatic Effects of Avocado Oil Using Single-cell Gel Electrophoresis (Comet Assay) |
| title_fullStr | Cytostatic Effects of Avocado Oil Using Single-cell Gel Electrophoresis (Comet Assay) |
| title_full_unstemmed | Cytostatic Effects of Avocado Oil Using Single-cell Gel Electrophoresis (Comet Assay) |
| title_short | Cytostatic Effects of Avocado Oil Using Single-cell Gel Electrophoresis (Comet Assay) |
| title_sort | cytostatic effects of avocado oil using single cell gel electrophoresis comet assay |
| topic | Avocado oil Persea Americana Genotoxicity Mutagenic Cytostatic |
| url | https://aro.koyauniversity.org/index.php/aro/article/view/1073 |
| work_keys_str_mv | AT subasiniuthirapathy cytostaticeffectsofavocadooilusingsinglecellgelelectrophoresiscometassay |